scholarly journals Construction of Drug Screening Cell Model and Application to New Compounds Interfering Production and Accumulation of Beta-Amyloid by Inhibiting Gamma-Secretase

10.5772/27974 ◽  
2011 ◽  
Author(s):  
Xiao-Ning Wang ◽  
Jie Yang ◽  
Ping-Yue Xu ◽  
Jie Chen ◽  
Dan Zhang ◽  
...  
Keyword(s):  
Drug Screening ◽  
Cell Model ◽  
Beta Amyloid ◽  
Gamma Secretase ◽  
New Compounds

scholarly journals Functional gamma-secretase inhibitors reduce beta-amyloid peptide levels in brain

2009 ◽  
Vol 76 (1) ◽  
pp. 173-181 ◽  
Author(s):  
H. F. Dovey ◽  
V. John ◽  
J. P. Anderson ◽  
L. Z. Chen ◽  
P. De Saint Andrieu ◽  
...  
Keyword(s):  
Beta Amyloid ◽  
Amyloid Peptide ◽  
Gamma Secretase ◽  
Beta Amyloid Peptide

scholarly journals IVIVC Assessment of Two Mouse Brain Endothelial Cell Models for Drug Screening

Pharmaceutics ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 587 ◽  
Author(s):  
Ina Puscas ◽  
Florian Bernard-Patrzynski ◽  
Martin Jutras ◽  
Marc-André Lécuyer ◽  
Lyne Bourbonnière ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Mrna Expression ◽  
Mouse Brain ◽  
Drug Screening ◽  
Cell Model ◽  
Drug Permeability ◽  
Mouse Brain Endothelial Cells ◽  
Primary Model

Since most preclinical drug permeability assays across the blood-brain barrier (BBB) are still evaluated in rodents, we compared an in vitro mouse primary endothelial cell model to the mouse b.End3 and the acellular parallel artificial membrane permeability assay (PAMPA) models for drug screening purposes. The mRNA expression of key feature membrane proteins of primary and bEnd.3 mouse brain endothelial cells were compared. Transwell® monolayer models were further characterized in terms of tightness and integrity. The in vitro in vivo correlation (IVIVC) was obtained by the correlation of the in vitro permeability data with log BB values obtained in mice for seven drugs. The mouse primary model showed higher monolayer integrity and levels of mRNA expression of BBB tight junction (TJ) proteins and membrane transporters (MBRT), especially for the efflux transporter Pgp. The IVIVC and drug ranking underlined the superiority of the primary model (r2 = 0.765) when compared to the PAMPA-BBB (r2 = 0.391) and bEnd.3 cell line (r2 = 0.019) models. The primary monolayer mouse model came out as a simple and reliable candidate for the prediction of drug permeability across the BBB. This model encompasses a rapid set-up, a fair reproduction of BBB tissue characteristics, and an accurate drug screening.

Simple Paper-based Liver Cell Model for Drug Screening

2020 ◽  
Vol 14 (2) ◽  
pp. 218-229
Author(s):  
Kattika Kaarj ◽  
Jennifer Ngo ◽  
Christina Loera ◽  
Patarajarin Akarapipad ◽  
Soohee Cho ◽  
...  
Keyword(s):  
Drug Screening ◽  
Liver Cell ◽  
Cell Model ◽  
Liver Cell Model

scholarly journals Spontaneously-forming spheroids as an in vitro cancer cell model for anticancer drug screening

Oncotarget ◽  
2015 ◽  
Vol 6 (25) ◽  
pp. 21255-21267 ◽  
Author(s):  
Maria A. Theodoraki ◽  
Celso O. Rezende ◽  
Oraphin Chantarasriwong ◽  
Adriana D. Corben ◽  
Emmanuel A. Theodorakis ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Anticancer Drug ◽  
Drug Screening ◽  
Cell Model

Testing Aβ toxicity on primary CNS cultures using drug-screening microfluidic chips

Lab on a Chip ◽  
2014 ◽  
Vol 14 (15) ◽  
pp. 2860-2866 ◽  
Author(s):  
A. Ruiz ◽  
P. Joshi ◽  
R. Mastrangelo ◽  
M. Francolini ◽  
C. Verderio ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Drug Screening ◽  
Primary Cultures ◽  
Beta Amyloid ◽  
Microfluidic Chips ◽  
Neuronal Viability ◽  
The Central Nervous System ◽  
Amyloid P ◽  
Neuroprotective Action

Neuronal viability tests performed in primary cultures and co-cultures of the central nervous system grown on microfluidic drug screening chips show a neuroprotective action of FTY720 in cultures degenerated by oligomeric beta amyloid.

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