scholarly journals Submergence Tolerance of Rice Species, Oryza glaberrima Steudel

10.5772/26023 ◽  
2012 ◽  
Author(s):  
Jun-Ichi Sakagami
Keyword(s):  
Oryza Glaberrima ◽  
Submergence Tolerance

scholarly journals Marker-Assisted Breeding of Thermo-Sensitive Genic Male Sterile Line 1892S for Disease Resistance and Submergence Tolerance

Rice Science ◽  
2021 ◽  
Vol 28 (1) ◽  
pp. 89-98
Author(s):  
Luo Yanchang ◽  
Ma Tingchen ◽  
Joanne Teo ◽  
Luo Zhixiang ◽  
Li Zefu ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Submergence Tolerance ◽  
Marker Assisted Breeding ◽  
Male Sterile ◽  
Male Sterile Line

scholarly journals Improved Transformation and Regeneration of Indica Rice: Disruption of SUB1A as a Test Case via CRISPR-Cas9

2021 ◽  
Vol 22 (13) ◽  
pp. 6989
Author(s):  
Yuya Liang ◽  
Sudip Biswas ◽  
Backki Kim ◽  
Julia Bailey-Serres ◽  
Endang M. Septiningsih
Keyword(s):  
Gene Editing ◽  
Indica Rice ◽  
Crop Improvement ◽  
Immature Embryo ◽  
Test Case ◽  
Submergence Tolerance ◽  
Embryogenic Calli ◽  
Submergence Stress ◽  
Efficient Vector ◽  
Vector Delivery

Gene editing by use of clustered regularly interspaced short palindromic repeats (CRISPR) has become a powerful tool for crop improvement. However, a common bottleneck in the application of this approach to grain crops, including rice (Oryza sativa), is efficient vector delivery and calli regeneration, which can be hampered by genotype-dependent requirements for plant regeneration. Here, methods for Agrobacterium-mediated and biolistic transformation and regeneration of indica rice were optimized using CRISPR-Cas9 gene-editing of the submergence tolerance regulator SUBMERGENCE 1A-1 gene of the cultivar Ciherang-Sub1. Callus induction and plantlet regeneration methods were optimized for embryogenic calli derived from immature embryos and mature seed-derived calli. Optimized regeneration (95%) and maximal editing efficiency (100%) were obtained from the immature embryo-derived calli. Phenotyping of T1 seeds derived from the edited T0 plants under submergence stress demonstrated inferior phenotype compared to their controls, which phenotypically validates the disruption of SUB1A-1 function. The methods pave the way for rapid CRISPR-Cas9 gene editing of recalcitrant indica rice cultivars.

scholarly journals Reproductive, pathogenic and genotypic characterisation of five Meloidogyne graminicola populations from the Philippines on susceptible and resistant rice varieties

Nematology ◽  
2018 ◽  
Vol 20 (4) ◽  
pp. 299-318
Author(s):  
Ma. Teodora Nadong Cabasan ◽  
Arvind Kumar ◽  
Stéphane Bellafiore ◽  
Dirk De Waele
Keyword(s):  
Phenotypic Variability ◽  
The Philippines ◽  
Growth And Yield ◽  
Oryza Glaberrima ◽  
Rice Varieties ◽  
Single Nucleotide ◽  
Nematode Reproduction ◽  
Rdna Genes ◽  
Meloidogyne Graminicola ◽  
Disease Induction

Summary Five populations of Meloidogyne graminicola isolated from different rice-growing areas in the Philippines were characterised. The populations showed little phenotypic variability of second-stage juveniles and female perineal pattern. Differences in reproduction among M. graminicola populations were not observed on mature resistant Oryza glaberrima varieties ‘TOG5674’, ‘TOG5675’, ‘RAM131’ and ‘CG14’, or on susceptible O. sativa varieties ‘IR64’ and ‘UPLRi-5’. In all infected rice varieties, plant growth and yield-contributing traits showed no differences among the populations. A search on M. graminicola populations from the Philippines for single-nucleotide polymorphism on the sequences of Internal Transcribed Spacer (ITS) of rDNA genes and mtDNA indicated only few points of heteroplasmy. Nematode reproduction and disease induction of the five M. graminicola populations in the Philippines exerted the same level of aggressiveness and virulence. The absence of resistance-breaking populations of M. graminicola is important for the maintenance of durability of resistance to this important rice pathogen.

scholarly journals Molecular characterization and genetic diversity of different genotypes of Oryza sativa and Oryza glaberrima

2017 ◽  
Vol 30 ◽  
pp. 48-57 ◽  
Author(s):  
Caijin Chen ◽  
Wenchuang He ◽  
Tondi Yacouba Nassirou ◽  
Athanase Nsabiyumva ◽  
Xilong Dong ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Oryza Sativa ◽  
Molecular Characterization ◽  
Oryza Glaberrima
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