scholarly journals New Therapeutic Strategies in Small Cell Lung Cancer: The Stem Cell Target

10.5772/25977 ◽  
2012 ◽  
Author(s):  
Guadalupe Aparicio ◽  
Vanessa Medina ◽  
Guillermo Alonso ◽  
L. Antn
Keyword(s):  
Lung Cancer ◽  
Stem Cell ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Therapeutic Strategies ◽  
Small Cell ◽  
Small Cell Lung ◽  
Cell Target

lncRNAs as Potential Targets in Small Cell Lung Cancer: MYC -dependent Regulation

2020 ◽  
Vol 20 (17) ◽  
pp. 2074-2081
Author(s):  
Onur Tokgun ◽  
Pervin E. Tokgun ◽  
Kubilay Inci ◽  
Hakan Akca
Keyword(s):  
Lung Cancer ◽  
Stem Cell ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Therapeutic Strategy ◽  
Small Cell ◽  
Small Cell Lung ◽  
Expression Levels ◽  
Qrt Pcr ◽  
Shrna Vector

Background: Small Cell Lung Cancer (SCLC) is a highly aggressive malignancy. MYC family oncogenes are amplified and overexpressed in 20% of SCLCs, showing that MYC oncogenes and MYC regulated genes are strong candidates as therapeutic targets for SCLC. c-MYC plays a fundamental role in cancer stem cell properties and malignant transformation. Several targets have been identified by the activation/repression of MYC. Deregulated expression levels of lncRNAs have also been observed in many cancers. Objective: The aim of the present study is to investigate the lncRNA profiles which depend on MYC expression levels in SCLC. Methods: Firstly, we constructed lentiviral vectors for MYC overexpression/inhibition. MYC expression is suppressed by lentiviral shRNA vector in MYC amplified H82 and N417 cells, and overexpressed by lentiviral inducible overexpression vector in MYC non-amplified H345 cells. LncRNA cDNA is transcribed from total RNA samples, and 91 lncRNAs are evaluated by qRT-PCR. Results: We observed that N417, H82 and H345 cells require MYC for their growth. Besides, MYC is not only found to regulate the expressions of genes related to invasion, stem cell properties, apoptosis and cell cycle (p21, Bcl2, cyclinD1, Sox2, Aldh1a1, and N-Cadherin), but also found to regulate lncRNAs. With this respect, expressions of AK23948, ANRIL, E2F4AS, GAS5, MEG3, H19, L1PA16, SFMBT2, ZEB2NAT, HOTAIR, Sox2OT, PVT1, and BC200 were observed to be in parallel with MYC expression, whereas expressions of Malat1, PTENP1, Neat1, UCA1, SNHG3, and SNHG6 were inversely correlated. Conclusion: Targeting MYC-regulated genes as a therapeutic strategy can be important for SCLC therapy. This study indicated the importance of identifying MYC-regulated lncRNAs and that these can be utilized to develop a therapeutic strategy for SCLC.

scholarly journals Synergistic Roles of Curcumin in Sensitising the Cisplatin Effect on a Cancer Stem Cell-Like Population Derived from Non-Small Cell Lung Cancer Cell Lines

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1056 ◽  
Author(s):  
Nazilah Abdul Satar ◽  
Mohd Nazri Ismail ◽  
Badrul Hisham Yahaya
Keyword(s):  
Lung Cancer ◽  
Stem Cell ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Cellular Proliferation ◽  
Combined Treatment ◽  
Small Cell ◽  
Cytotoxic Agents ◽  
Small Cell Lung ◽  
Proliferation Activity

Cancer stem cells (CSCs) represent a small subpopulation within a tumour. These cells possess stem cell-like properties but also initiate resistance to cytotoxic agents, which contributes to cancer relapse. Natural compounds such as curcumin that contain high amounts of polyphenols can have a chemosensitivity effect that sensitises CSCs to cytotoxic agents such as cisplatin. This study was designed to investigate the efficacy of curcumin as a chemo-sensitiser in CSCs subpopulation of non-small cell lung cancer (NSCLC) using the lung cancer adenocarcinoma human alveolar basal epithelial cells A549 and H2170. The ability of curcumin to sensitise lung CSCs to cisplatin was determined by evaluating stemness characteristics, including proliferation activity, colony formation, and spheroid formation of cells treated with curcumin alone, cisplatin alone, or the combination of both at 24, 48, and 72 h. The mRNA level of genes involved in stemness was analysed using quantitative real-time polymerase chain reaction. Liquid chromatography-mass spectrometry was used to evaluate the effect of curcumin on the CSC niche. A combined treatment of A549 subpopulations with curcumin reduced cellular proliferation activity at all time points. Curcumin significantly (p < 0.001) suppressed colonies formation by 50% and shrank the spheroids in CSC subpopulations, indicating inhibition of their self-renewal capability. This effect also was manifested by the down-regulation of SOX2, NANOG, and KLF4. Curcumin also regulated the niche of CSCs by inhibiting chemoresistance proteins, aldehyde dehydrogenase, metastasis, angiogenesis, and proliferation of cancer-related proteins. These results show the potential of using curcumin as a therapeutic approach for targeting CSC subpopulations in non-small cell lung cancer.

scholarly journals Using stem cell biology to design precision medicine for non-small cell lung cancer

2016 ◽  
Vol 11 (2) ◽  
pp. S4-S5
Author(s):  
Christine M. Fillmore ◽  
Chunxiao Xu ◽  
Francisco J. Sánchez-Rivera ◽  
Tyler Jacks ◽  
Kwok-Kin Wong ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Stem Cell ◽  
Small Cell Lung Cancer ◽  
Precision Medicine ◽  
Cell Lung Cancer ◽  
Cell Biology ◽  
Small Cell ◽  
Stem Cell Biology ◽  
Small Cell Lung

Cancer Stem Cell Biomarkers in EGFR-Mutation–Positive Non–Small-Cell Lung Cancer

2019 ◽  
Vol 20 (3) ◽  
pp. 167-177 ◽  
Author(s):  
Jordi Codony-Servat ◽  
Carles Codony-Servat ◽  
Andrés Felipe Cardona ◽  
Ana Giménez-Capitán ◽  
Ana Drozdowskyj ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Egfr Mutation ◽  
Small Cell ◽  
Small Cell Lung

scholarly journals Putative Stem Cell Markers in Non–Small-Cell Lung Cancer: A Clinicopathologic Characterization

2014 ◽  
Vol 9 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Willam Sterlacci ◽  
Spasenija Savic ◽  
Michael Fiegl ◽  
Ellen Obermann ◽  
Alexandar Tzankov
Keyword(s):  
Lung Cancer ◽  
Stem Cell ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Stem Cell Markers ◽  
Small Cell Lung ◽  
Cell Markers ◽  
Putative Stem Cell

scholarly journals Interactions of cancer stem cell and immune microenvironment in non-small cell lung cancer (NSCLC)

2018 ◽  
Vol 29 ◽  
pp. viii660-viii661
Author(s):  
F. Zhang ◽  
N. Dong ◽  
S. Torres ◽  
S. Calabuig Fariñas ◽  
R. Sirera Perez ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Small Cell Lung ◽  
Immune Microenvironment
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