scholarly journals Neutrophil Activation and Erythrocyte Membrane Protein Composition in Stage 5 Chronic Kidney Disease Patients

10.5772/25530 ◽  
2012 ◽  
Author(s):  
Elsio Costa ◽  
Lus Belo ◽  
Alice Santos-Silv
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Membrane Protein ◽  
Erythrocyte Membrane ◽  
Protein Composition ◽  
Neutrophil Activation ◽  
Erythrocyte Membrane Protein

Altered erythrocyte membrane protein composition mirrors pleiotropic effects of hypertension susceptibility genes and disease pathogenesis

2015 ◽  
Vol 33 (11) ◽  
pp. 2265-2277 ◽  
Author(s):  
Alexey V. Polonikov ◽  
Dmitry V. Ushachev ◽  
Vladimir P. Ivanov ◽  
Mikhail I. Churnosov ◽  
Maxim B. Freidin ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Erythrocyte Membrane ◽  
Protein Composition ◽  
Susceptibility Genes ◽  
Pleiotropic Effects ◽  
Disease Pathogenesis ◽  
Erythrocyte Membrane Protein

Alterations in erythrocyte membrane protein composition in advanced non-small cell lung cancer

2006 ◽  
Vol 36 (3) ◽  
pp. 355-363 ◽  
Author(s):  
Angel Hernández-Hernández ◽  
Marina C. Rodríguez ◽  
Abel López-Revuelta ◽  
José I. Sánchez-Gallego ◽  
Valery Shnyrov ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Membrane Protein ◽  
Small Cell Lung Cancer ◽  
Erythrocyte Membrane ◽  
Cell Lung Cancer ◽  
Protein Composition ◽  
Small Cell ◽  
Small Cell Lung ◽  
Erythrocyte Membrane Protein

Identifying targets of protective antibodies against severe malaria in Papua, Indonesia using locally expressed domains of Plasmodium falciparum Erythrocyte Membrane Protein 1.

2021 ◽  
Author(s):  
Janavi S Rambhatla ◽  
Gerry Q Tonkin-Hill ◽  
Eizo Takashima ◽  
Takafumi Tsuboi ◽  
Rintis Noviyanti ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Membrane Protein ◽  
Severe Malaria ◽  
Erythrocyte Membrane ◽  
Uncomplicated Malaria ◽  
Igg Antibodies ◽  
Erythrocyte Membrane Protein ◽  
African Children ◽  
Genes Encoding ◽  
Malaria Severity

Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1), a diverse family of multi-domain proteins expressed on the surface of malaria-infected erythrocytes, is an important target of protective immunity against malaria. Our group recently studied transcription of the var genes encoding PfEMP1 in individuals from Papua, Indonesia with severe or uncomplicated malaria. We cloned and expressed domains from 32 PfEMP1s including 22 that were upregulated in severe malaria and 10 that were upregulated in uncomplicated malaria, using a wheat germ cell-free expression system. We used Luminex technology to measure IgG antibodies to these 32 domains and control proteins in 63 individuals (11 children). At presentation to hospital, levels of antibodies to PfEMP1 domains were either higher in uncomplicated malaria or were not significantly different between groups. Using principal components analysis, antibodies to three of 32 domains were highly discriminatory between groups. These included two domains upregulated in severe malaria, a DBLβ13 domain and a CIDRα1.6 domain (which has been previously implicated in severe malaria pathogenesis), and a DBLδ domain that was upregulated in uncomplicated malaria. Antibody to control non-PfEMP1 antigens did not differ with disease severity. Antibodies to PfEMP1 domains differ with malaria severity. Lack of antibodies to locally expressed PfEMP1 types, including both domains previously associated with severe malaria and newly identified targets, may in part explain malaria severity in Papuan adults. Importance Severe Plasmodium falciparum malaria kills many African children, and lack of antibody immunity predisposes to severe disease. A critical antibody target is the P. falciparum erythrocyte membrane 1 (PfEMP1) family of multidomain proteins, which are expressed on the infected erythrocyte surface and mediate parasite sequestration in deep organs. We previously identified var genes encoding PfEMP1 that were differentially expressed between severe and uncomplicated malaria in Papua, Indonesia. Here, we have expressed domains from 32 of these PfEMP1s and measured IgG antibody responses to them in Papuan adults and children. Using Principal Component Analysis, IgG antibodies to three domains distinguished between severe and uncomplicated malaria and were higher in uncomplicated malaria. Domains included CIDRα1.6, implicated in severe malaria; a DBLβ13 domain; and a DBLδ domain of unknown function. Immunity to locally relevant PfEMP1 domains may protect from severe malaria. Targets of immunity show important overlap between Asian adults and African children.

Carbamylation of normal and uremic erythrocyte membrane protein

1995 ◽  
Vol 28 (3) ◽  
pp. 332 ◽  
Author(s):  
Daniel J. Trepanier ◽  
Patrick S. Caines ◽  
Roger J. Thibert ◽  
Michael Goodwin ◽  
Thomas F. Draisey
Keyword(s):  
Membrane Protein ◽  
Erythrocyte Membrane ◽  
Erythrocyte Membrane Protein

scholarly journals DNA secondary structures are associated with recombination in major Plasmodium falciparum variable surface antigen gene families

2013 ◽  
Vol 42 (4) ◽  
pp. 2270-2281 ◽  
Author(s):  
Adam F. Sander ◽  
Thomas Lavstsen ◽  
Thomas S. Rask ◽  
Michael Lisby ◽  
Ali Salanti ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Membrane Protein ◽  
Erythrocyte Membrane ◽  
Gene Families ◽  
Secondary Structures ◽  
Erythrocyte Membrane Protein ◽  
Sexual Stages ◽  
Dna Secondary Structures ◽  
Parasitic Pathogens ◽  
Falciparum Erythrocyte Membrane Protein

Abstract Many bacterial, viral and parasitic pathogens undergo antigenic variation to counter host immune defense mechanisms. In Plasmodium falciparum, the most lethal of human malaria parasites, switching of var gene expression results in alternating expression of the adhesion proteins of the Plasmodium falciparum-erythrocyte membrane protein 1 class on the infected erythrocyte surface. Recombination clearly generates var diversity, but the nature and control of the genetic exchanges involved remain unclear. By experimental and bioinformatic identification of recombination events and genome-wide recombination hotspots in var genes, we show that during the parasite’s sexual stages, ectopic recombination between isogenous var paralogs occurs near low folding free energy DNA 50-mers and that these sequences are heavily concentrated at the boundaries of regions encoding individual Plasmodium falciparum-erythrocyte membrane protein 1 structural domains. The recombinogenic potential of these 50-mers is not parasite-specific because these sequences also induce recombination when transferred to the yeast Saccharomyces cerevisiae. Genetic cross data suggest that DNA secondary structures (DSS) act as inducers of recombination during DNA replication in P. falciparum sexual stages, and that these DSS-regulated genetic exchanges generate functional and diverse P. falciparum adhesion antigens. DSS-induced recombination may represent a common mechanism for optimizing the evolvability of virulence gene families in pathogens.

Sign in / Sign up

Export Citation Format

Share Document