scholarly journals Application of Agrobacterium Rol Genes in Plant Biotechnology: A Natural Phenomenon of Secondary Metabolism Regulation

10.5772/24200 ◽  
2011 ◽  
Author(s):  
Victor P ◽  
Yuri N ◽  
Galina N ◽  
Tatiana Y ◽  
Yuliya V
Keyword(s):  
Secondary Metabolism ◽  
Plant Biotechnology ◽  
Rol Genes ◽  
Natural Phenomenon ◽  
Metabolism Regulation

scholarly journals Feasible strategies for studying the involvement of DNA methylation and histone acetylation in the stress-induced formation of quality-related metabolites in tea (Camellia sinensis)

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jie Yang ◽  
Dachuan Gu ◽  
Shuhua Wu ◽  
Xiaochen Zhou ◽  
Jiaming Chen ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Histone Acetylation ◽  
Secondary Metabolism ◽  
Regulatory Mechanisms ◽  
Epigenetic Mechanisms ◽  
Research Strategies ◽  
Metabolism Regulation ◽  
Growth Development ◽  
Regulatory Effects ◽  
Tea Plants

AbstractTea plants are subjected to multiple stresses during growth, development, and postharvest processing, which affects levels of secondary metabolites in leaves and influences tea functional properties and quality. Most studies on secondary metabolism in tea have focused on gene, protein, and metabolite levels, whereas upstream regulatory mechanisms remain unclear. In this review, we exemplify DNA methylation and histone acetylation, summarize the important regulatory effects that epigenetic modifications have on plant secondary metabolism, and discuss feasible research strategies to elucidate the underlying specific epigenetic mechanisms of secondary metabolism regulation in tea. This information will help researchers investigate the epigenetic regulation of secondary metabolism in tea, providing key epigenetic data that can be used for future tea genetic breeding.

scholarly journals Roles of <italic>SmCOI1</italic> in pest resistance and secondary metabolism regulation based on <italic>Salvia miltiorrhiza</italic> Bunge genome

2018 ◽  
Vol 48 (4) ◽  
pp. 399-411
Author(s):  
ZheZhi WANG ◽  
Ying HUANG ◽  
XiaoYan CAO ◽  
Chen CHEN ◽  
Yuan ZHANG ◽  
...  
Keyword(s):  
Secondary Metabolism ◽  
Salvia Miltiorrhiza ◽  
Pest Resistance ◽  
Metabolism Regulation

Manipulation of secondary metabolism in cultured plant cells by agrobacterium rol genes

2008 ◽  
Vol 136 ◽  
pp. S131
Author(s):  
Victor P. Bulgakov ◽  
Sergey A. Fedoreyev ◽  
Konstantin V. Kiselev ◽  
Yuri N. Shkryl ◽  
Yulia V. Inyushkina ◽  
...  
Keyword(s):  
Secondary Metabolism ◽  
Plant Cells ◽  
Rol Genes

scholarly journals The kinetochore protein Spc105, a novel interaction partner of LaeA, regulates development and secondary metabolism in Aspergillus flavus

2018 ◽  
Author(s):  
QingQing Zhi ◽  
Lei He ◽  
JieYing Li ◽  
Jing Li ◽  
ZhenLong Wang ◽  
...  
Keyword(s):  
Secondary Metabolism ◽  
Aspergillus Flavus ◽  
Leucine Zipper ◽  
Cyclopiazonic Acid ◽  
Gene Clusters ◽  
Interaction Partner ◽  
Kinetochore Protein ◽  
Metabolism Regulation ◽  
Fungal Secondary Metabolism

Nuclear protein LaeA is known as the global regulator of secondary metabolism in Aspergillus. LaeA connects with VeA and VelB to form a heterotrimeric complex, which coordinates fungal development and secondary metabolism. Here, we describe a new interaction partner of LaeA, the kinetochore protein Spc105, from the aflatoxin-producing fungus Aspergillus flavus. We showed that in addition to involvement in nuclear division, Spc105 is required for normal conidiophore development and sclerotia production of A. flavus. Moreover, Spc105 positively regulates the production of secondary metabolites such as aflatoxin and kojic acid, and negatively regulates the production of cyclopiazonic acid. Transcriptome analysis of the ?spc105 strain revealed that 23 backbone genes were differentially expressed, corresponding to 19 of the predicted 56 secondary metabolite gene clusters, suggesting a broad regulatory role of Spc105 in secondary metabolism. Notably, the reduced expression of laeA in our transcriptome data led to the discovery of the correlation between Spc105 and LaeA, and double mutant analysis indicated a functional interdependence between Spc105 and LaeA. Further, yeast two-hybrid (Y2H) and GST pull-down assays revealed that Spc105 interacts directly with the S-adenosylmethionine (SAM)-binding domain of LaeA, and that the leucine zipper motif in Spc105 is required for this interaction. The Spc105-LaeA interaction identified in our study indicates a cooperative interplay of distinct regulators in A. flavus, providing new insights into fungal secondary metabolism regulation networks.

Proteomics in plant biotechnology and secondary metabolism research

2000 ◽  
Vol 11 (5) ◽  
pp. 277-287 ◽  
Author(s):  
Denise I. Jacobs ◽  
Robert van der Heijden ◽  
Robert Verpoorte
Keyword(s):  
Secondary Metabolism ◽  
Plant Biotechnology

scholarly journals Exploration of the Effects of Different Blue LED Light Intensities on Flavonoid and Lipid Metabolism in Tea Plants via Transcriptomics and Metabolomics

2020 ◽  
Vol 21 (13) ◽  
pp. 4606
Author(s):  
Pengjie Wang ◽  
Sirong Chen ◽  
Mengya Gu ◽  
Xiaomin Chen ◽  
Xuejin Chen ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Secondary Metabolism ◽  
Blue Light ◽  
High Intensity ◽  
Future Research ◽  
Hub Gene ◽  
Metabolism Regulation ◽  
Physiological Processes ◽  
Light Quantity ◽  
Tea Plants

Blue light extensively regulates multiple physiological processes and secondary metabolism of plants. Although blue light quantity (fluence rate) is important for plant life, few studies have focused on the effects of different blue light intensity on plant secondary metabolism regulation, including tea plants. Here, we performed transcriptomic and metabolomic analyses of young tea shoots (one bud and two leaves) under three levels of supplemental blue light, including low-intensity blue light (LBL, 50 μmol m–2 s–1), medium-intensity blue light (MBL, 100 μmol m–2 s–1), and high-intensity blue light (HBL, 200 μmol m–2 s–1). The total number of differentially expressed genes (DEGs) in LBL, MBL and HBL was 1, 7 and 1097, respectively, indicating that high-intensity blue light comprehensively affects the transcription of tea plants. These DEGs were primarily annotated to the pathways of photosynthesis, lipid metabolism and flavonoid synthesis. In addition, the most abundant transcription factor (TF) families in DEGs were bHLH and MYB, which have been shown to be widely involved in the regulation of plant flavonoids. The significantly changed metabolites that we detected contained 15 lipids and 6 flavonoid components. Further weighted gene co-expression network analysis (WGCNA) indicated that CsMYB (TEA001045) may be a hub gene for the regulation of lipid and flavonoid metabolism by blue light. Our results may help to establish a foundation for future research investigating the regulation of woody plants by blue light.

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