Calorimetric Investigations of Non-Viral DNA Transfection Systems

YMDD motif in hepatitis B virus DNA polymerase influences on replication and lamivudine resistance: A study byin vitrofull-length viral DNA transfection

Hepatology ◽

10.1002/hep.510290340 ◽

1999 ◽

Vol 29 (3) ◽

pp. 939-945 ◽

Cited By ~ 119

Author(s):

Suzane Kioko Ono-Nita ◽

Naoya Kato ◽

Yasushi Shiratori ◽

Tsutomu Masaki ◽

Keng-Hsin Lan ◽

...

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Dna Polymerase ◽

Dna Transfection ◽

Lamivudine Resistance ◽

Viral Dna ◽

Hepatitis B Virus Dna ◽

B Virus ◽

Ymdd Motif

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Optimization of human papillomavirus-based pseudovirus techniques for efficient gene transfer

10.1101/2020.04.30.070573 ◽

2020 ◽

Author(s):

Timra D. Gilson ◽

Ryan T. Gibson ◽

Elliot J. Androphy

Keyword(s):

Human Papillomavirus ◽

Gene Transfer ◽

Cell Types ◽

Extracellular Matrices ◽

Dna Transfection ◽

Viral Dna ◽

Infection Efficiency ◽

Efficient Gene ◽

L1 And L2 ◽

Different Cell Types

AbstractHuman papillomavirus (HPV) L1 and L2 capsid proteins self-assemble into virions capable of efficiently packaging either its 8 kilobase genome or non-viral DNA. The ability of HPV capsids to package non-viral DNA makes these a useful tool for delivering plasmids to study proteins of interest in a variety of cell types. We describe optimization of current methods and present new protocols for using HPV capsids to deliver non-viral DNA thereby providing an alternative to DNA transfection. Using keratinocyte generated extracellular matrices can enhance infection efficiency in keratinocytes, hepatocytes and neuronal cells. Furthermore, we describe a suspension-based efficient technique for infecting different cell types.

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Optimization of human papillomavirus-based pseudovirus techniques for efficient gene transfer

Scientific Reports ◽

10.1038/s41598-020-72027-1 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Timra D. Gilson ◽

Ryan T. Gibson ◽

Elliot J. Androphy

Keyword(s):

Human Papillomavirus ◽

Gene Transfer ◽

Cell Types ◽

Extracellular Matrices ◽

Dna Transfection ◽

Viral Dna ◽

Infection Efficiency ◽

Efficient Gene ◽

L1 And L2 ◽

Different Cell Types

Abstract Human papillomavirus (HPV) L1 and L2 capsid proteins self-assemble into virions capable of efficiently packaging either its 8 kilobase genome or non-viral DNA. The ability of HPV capsids to package non-viral DNA makes these a useful tool for delivering plasmids to study proteins of interest in a variety of cell types. We describe optimization of current methods and present new protocols for using HPV capsids to deliver non-viral DNA thereby providing an alternative to DNA transfection. Using keratinocyte generated extracellular matrices can enhance infection efficiency in keratinocytes, hepatocytes and neuronal cells. Furthermore, we describe a suspension-based efficient technique for infecting different cell types.

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The Study on Poly-Lysine-Alginate Microcapsules Mediated Virus Genomic DNA Transfection

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.699.298 ◽

2013 ◽

Vol 699 ◽

pp. 298-301

Author(s):

Guo Qing Wang ◽

Dan Zhang ◽

Liu Yuan Xia ◽

Nai Sheng Zhang

Keyword(s):

Biological Activity ◽

Genomic Dna ◽

Intramuscular Injection ◽

Pseudorabies Virus ◽

Dna Transfection ◽

Viral Dna

The goal in this paper is to investigate the efficiency of poly-PLL (Poly-L-lysine)/ Alg (Alginate) vector mediated virus genomic DNA transfection and the virus genomic DNA’s biological activity in vivo. After Pseudorabies virus (PRV) genomic DNA being adhered to the porous СаСО3 particles, PLL and Alg were alternately polymerized on the surface of the porous DNA-СаСО3 particles to 7 layers, which were later dissolved them by EDTA to remove СаСО3 cores; the vectors in which the DNA were coated by poly-PLL/Alg, were harvested to infect the rabbits and observe the replication of viral DNA. Porous СаСО3 particles, which were obtained from the reaction between Na2CO3 and CaCl2, had an efficiency of absorbing DNA 1 µg/mg СаСО3 particles. After being coated by PLL/Alg, microcapsules were obtained with the diameter of 2-4 µm. 10.0 µg of poly-PLL/Alg-PRV DNA microcapsules could cause rabbits’ death by intramuscular injection. The identification of PCR shows that the death was caused by PRV infection. The results indicate that Poly-PLL/Alg microcapsules can mediate efficient transfection of DNA.

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Use of Antibody to aid Visualization of Protein at the Termini of Bacteriophage Ø29 DNA

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600002385 ◽

1980 ◽

Vol 38 ◽

pp. 540-541

Author(s):

Dwight Anderson ◽

Charlene Peterson ◽

Gursaran Notani ◽

Bernard Reilly

Keyword(s):

Electron Microscopy ◽

Bacillus Subtilis ◽

Dna Synthesis ◽

Restriction Endonuclease ◽

Protein Complex ◽

Protein Product ◽

Viral Dna ◽

Small Proteins ◽

Antibody Labeling ◽

Subtilis Bacteriophage

The protein product of cistron 3 of Bacillus subtilis bacteriophage Ø29 is essential for viral DNA synthesis and is covalently bound to the 5’-termini of the Ø29 DNA. When the DNA-protein complex is cleaved with a restriction endonuclease, the protein is bound to the two terminal fragments. The 28,000 dalton protein can be visualized by electron microscopy as a small dot and often is seen only when two ends are in apposition as in multimers or in glutaraldehyde-fixed aggregates. We sought to improve the visibility of these small proteins by use of antibody labeling.

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