scholarly journals Image Processing Methods for Automatic Cell Counting In Vivo or In Situ Using 3D Confocal Microscopy

10.5772/23147 ◽  
2011 ◽  
Author(s):  
Manuel G. ◽  
Alicia Hidalgo
Keyword(s):  
Image Processing ◽  
Confocal Microscopy ◽  
Cell Counting ◽  
Processing Methods

Evaluation of corneal stromal changes in vivo after laser in situ keratomileusis with confocal microscopy

Ophthalmology ◽  
2001 ◽  
Vol 108 (10) ◽  
pp. 1744-1750 ◽  
Author(s):  
Pierre-Jean Pisella ◽  
Olivier Auzerie ◽  
Yves Bokobza ◽  
Caroline Debbasch ◽  
Christophe Baudouin
Keyword(s):  
Confocal Microscopy ◽  
Laser In Situ Keratomileusis

scholarly journals LncRNA AC245100.4 binds HSP90 to promote the proliferation of prostate cancer

Epigenomics ◽  
2020 ◽  
Vol 12 (15) ◽  
pp. 1257-1271
Author(s):  
Rongjun Cui ◽  
Chi Liu ◽  
Ping Lin ◽  
Hui Xie ◽  
Wei Wang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Counting ◽  
Chaperone Function ◽  
In Vivo Assays ◽  
Iκb Kinase ◽  
Rna Immunoprecipitation ◽  
The Stability

Aim: To investigate the role and mechanisms of AC245100.4 in prostate cancer. Materials & methods: The expression and location of AC245100.4 were examined using real-time PCR and  in situ hybridization. Cell Counting Kit-8, clone formation, flow cytometry and in vivo assays were conducted to determine the role of AC245100.4. RNA antisense purification with mass spectrometry and RNA immunoprecipitation were performed to identify proteins that bind to AC245100.4. Western blotting was performed to quantify the expression of protein. Results: AC245100.4 expression was upregulated in prostate cancer and mainly located in the cytoplasm. Knockdown of AC245100.4 inhibited proliferation of prostate cancer. Mechanistically, AC245100.4 bound to HSP90 and altered its chaperone function, increased the stability of IκB kinase and activated the NFκB signaling pathway. Conclusion: AC245100.4 promotes the proliferation of prostate cancer via binding of HSP90.

Parallel in-situ measurement of heliostat aim points in central receiver systems by image processing methods

Solar Energy ◽  
2019 ◽  
Vol 180 ◽  
pp. 648-663 ◽  
Author(s):  
Gregor Bern ◽  
Peter Schöttl ◽  
De Wet van Rooyen ◽  
Anna Heimsath ◽  
Peter Nitz
Keyword(s):  
Image Processing ◽  
In Situ Measurement ◽  
Processing Methods ◽  
Central Receiver

Distribution, function, and prognostic value of cytotoxic T lymphocytes in follicular lymphoma: a 3-D tissue-imaging study

Blood ◽  
2011 ◽  
Vol 118 (20) ◽  
pp. 5371-5379 ◽  
Author(s):  
Camille Laurent ◽  
Sabina Müller ◽  
Catherine Do ◽  
Talal Al-Saati ◽  
Sophie Allart ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Lymph Nodes ◽  
Follicular Lymphoma ◽  
Imaging Study ◽  
Progression Free Survival ◽  
Tissue Imaging ◽  
Combined Chemotherapy ◽  
3 Dimensional

Abstract CD8+ CTLs are thought to play a role in the control of follicular lymphoma (FL). Yet, the link between CTL tissue distribution, activation status, ability to kill FL cells in vivo, and disease progression is still elusive. Pretreatment lymph nodes from FL patients were analyzed by IHC (n = 80) or by 3-color confocal microscopy (n = 10). IHC revealed a rich infiltrate of CD8+ granzyme B+ (GrzB) cells in FL interfollicular spaces. Accordingly, confocal microscopy showed an increased number of CD3+CD8+GrzB+ CTLs and a brighter GrzB staining in individual CTL in FL samples compared with reactive lymph nodes. CTLs did not penetrate tumor nodules. In 3-dimensional (3-D) image reconstructions, CTLs were detected at the FL follicle border where they formed lytic synapse-like structures with FL B cells and with apoptotic cells, suggesting an in situ cytotoxic function. Finally, although GrzB expression in CTLs did not correlate with risk factors, high GrzB content correlated with prolonged progression free-survival (PFS) after rituximab-combined chemotherapy. Our results show the recruitment of armed CTLs with a tumor-controlling potential into FL lymph nodes and suggest that CTL-associated GrzB expression could influence PFS in FL patients having received rituximab-combined chemotherapy.

In vivo confocal microscopy through-focusing to measure corneal flap thickness after laser in situ keratomileusis

2002 ◽  
Vol 28 (6) ◽  
pp. 962-970 ◽  
Author(s):  
Fusun Gokmen ◽  
James V. Jester ◽  
Matthew W. Petroll ◽  
James P. McCulley ◽  
Dwight H. Cavanagh
Keyword(s):  
Confocal Microscopy ◽  
Laser In Situ Keratomileusis ◽  
In Vivo Confocal Microscopy ◽  
Flap Thickness

Surgical Margin Mapping of Melanoma In Situ Using In Vivo Reflectance Confocal Microscopy Mosaics

2021 ◽  
Vol 47 (5) ◽  
pp. 605-608
Author(s):  
John R. Durkin ◽  
Catherine N. Tchanque-Fossuo ◽  
Alexander N. Rose ◽  
Hillary R. Elwood ◽  
Shelly Stepenaskie ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Surgical Margin ◽  
Reflectance Confocal Microscopy ◽  
Melanoma In Situ

In situ techniques to predict in vivo digestibility and to evaluate the impact of flint maize processing methods on degradation parameters

2021 ◽  
pp. 1-11
Author(s):  
L. A. Godoi ◽  
B. C. Silva ◽  
A. C. B. Menezes ◽  
F. A. S. Silva ◽  
H. M. Alhadas ◽  
...  
Keyword(s):  
Dry Matter ◽  
Latin Square ◽  
High Moisture ◽  
Processing Methods ◽  
In Situ Techniques ◽  
Whole Plant ◽  
Maize Grain ◽  
The Impact

Abstract This study aimed to (1) evaluate the effects of flint maize processing methods on the estimation of the readily soluble fraction (a), the potentially degradable fraction (b) and the rate of degradation of b (c) for dry matter (DM), organic matter (OM) and starch in the rumen; and (2) verify whether two different applications of in situ technique can be used to estimate in vivo DM, OM and starch digestibilities. Five ruminally cannulated Nellore bulls (265 ± 18.2 kg; 8 ± 1.0 mo) were distributed in a 5 × 5 Latin square. Three experimental diets were composed of 0.30 whole-plant maize silage, 0.10 supplement and 0.60 of one of the following processing methods: dry ground maize grain (DMG); high-moisture maize (HMM); reconstituted maize grain silage (RMG). Two additional diets were composed of 0.10 supplement, 0.80 snaplage and 0.10 stalklage (SNAP-80); or 0.10 supplement and 0.90 snaplage (SNAP-90). Digestibilities were estimated using in vivo procedure or predicted from in situ technique using a single 24 h incubation point or an equation proposed in previous literature. Diets based on ensiled grains presented greater (P < 0.05) fraction a and c and lower (P < 0.05) fraction b of DM, OM and starch compared to DMG. Both alternative use of in situ technique accurately estimated (P > 0.05) in vivo DM, OM and starch digestibilities. The results suggest that ensilage process may increase the availability of nutrients. The two different applications of in situ technique showed precision and accuracy to estimate in vivo digestibility.

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