scholarly journals RNA Processing Activities of the Arabidopsis Argonaute Protein Family

10.5772/22686 ◽  
2011 ◽  
Author(s):  
Ki Wook ◽  
Andrew L ◽  
Peter M
Keyword(s):  
Rna Processing ◽  
Protein Family ◽  
Argonaute Protein

scholarly journals The Argonaute protein family

2008 ◽  
Vol 9 (2) ◽  
pp. 210 ◽  
Author(s):  
Julia Höck ◽  
Gunter Meister
Keyword(s):  
Protein Family ◽  
Argonaute Protein

Assembly and function of small RNA – Argonaute protein complexes

2014 ◽  
Vol 395 (6) ◽  
pp. 611-629 ◽  
Author(s):  
Anne Dueck ◽  
Gunter Meister
Keyword(s):  
Gene Expression ◽  
Gene Silencing ◽  
Small Rna ◽  
Small Rnas ◽  
Atp Hydrolysis ◽  
Protein Complexes ◽  
Protein Family ◽  
Argonaute Protein ◽  
Argonaute Proteins ◽  
And Function

Abstract Small RNAs such as microRNAs (miRNAs), short interfering RNAs (siRNAs) or Piwi-interacting RNAs (piRNAs) are important regulators of gene expression in various organisms. Small RNAs bind to a member of the Argonaute protein family and are incorporated into larger structures that mediate diverse gene silencing events. The loading of Argonaute proteins with small RNAs is aided by a number of auxiliary factors as well as ATP hydrolysis. This review will focus on the mechanisms of Argonaute loading in different organisms. Furthermore, we highlight the versatile functions of small RNA-Argonaute protein complexes in organisms from all three kingdoms of life.

scholarly journals A Primary Sequence Analysis of the ARGONAUTE Protein Family in Plants

2016 ◽  
Vol 7 ◽  
Author(s):  
Daniel Rodríguez-Leal ◽  
Amanda Castillo-Cobián ◽  
Isaac Rodríguez-Arévalo ◽  
Jean-Philippe Vielle-Calzada
Keyword(s):  
Sequence Analysis ◽  
Protein Family ◽  
Argonaute Protein ◽  
Primary Sequence ◽  
Primary Sequence Analysis

scholarly journals siRNA Specificity: RNAi Mechanisms and Strategies to Reduce Off-Target Effects

2021 ◽  
Vol 11 ◽  
Author(s):  
Julia Neumeier ◽  
Gunter Meister
Keyword(s):  
Rna Interference ◽  
Protein A ◽  
Crop Protection ◽  
Protein Family ◽  
Argonaute Protein ◽  
Guide Strand ◽  
Rna Modifications ◽  
Double Stranded Rna ◽  
Target Rna ◽  
Target Effects

Short interfering RNAs (siRNAs) are processed from long double-stranded RNA (dsRNA), and a guide strand is selected and incorporated into the RNA-induced silencing complex (RISC). Within RISC, a member of the Argonaute protein family directly binds the guide strand and the siRNA guides RISC to fully complementary sites on-target RNAs, which are then sequence-specifically cleaved by the Argonaute protein—a process commonly referred to as RNA interference (RNAi). In animals, endogenous microRNAs (miRNAs) function similarly but do not lead to direct cleavage of the target RNA but to translational inhibition followed by exonucleolytic decay. This is due to only partial complementarity between the miRNA and the target RNA. SiRNAs, however, can function as miRNAs, and partial complementarity can lead to miRNA-like off-target effects in RNAi applications. Since siRNAs are widely used not only for screening but also for therapeutics as well as crop protection purposes, such miRNA-like off-target effects need to be minimized. Strategies such as RNA modifications or pooling of siRNAs have been developed and are used to reduce off-target effects.

Analysis of AgnA, a member of the argonaute protein family in Dictyostelium discoideum

2005 ◽  
Vol 2005 (Fall) ◽  
Author(s):  
Xiaoxiao Zhang ◽  
Markus Kaller ◽  
Wolfgang Nellen ◽  
Christian Hammann
Keyword(s):  
Dictyostelium Discoideum ◽  
Protein Family ◽  
Argonaute Protein

SAT0565 The Expression and Regulation of the Argonaute Protein Family Member PIWIL4 in RA

2014 ◽  
Vol 73 (Suppl 2) ◽  
pp. 795.1-795
Author(s):  
L. Pleštilová ◽  
B. Aradi ◽  
M. Filková ◽  
L. Šenolt ◽  
A. Ciurea ◽  
...  
Keyword(s):  
Family Member ◽  
Protein Family ◽  
Argonaute Protein
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