scholarly journals Reverse Transcriptase and Retroviral Replication

10.5772/21660 ◽  
2011 ◽  
Author(s):  
T. Matamoros ◽  
M. Alvarez ◽  
V. Barrioluengo ◽  
G. Betancor ◽  
L. Menendez-Arias
Keyword(s):  
Reverse Transcriptase ◽  
Retroviral Replication

Reverse Transcriptase and Retroviral Replication

2004 ◽  
pp. 708-713
Author(s):  
Laura Tarrago-Litvak ◽  
Marie-Line Andréola ◽  
Simon Litvak
Keyword(s):  
Reverse Transcriptase ◽  
Retroviral Replication

scholarly journals In Vivo Ty1 Reverse Transcription Can Generate Replication Intermediates with Untidy Ends

1998 ◽  
Vol 72 (8) ◽  
pp. 6490-6503 ◽  
Author(s):  
Emilie H. Mules ◽  
Ozcan Uzun ◽  
Abram Gabriel
Keyword(s):  
Reverse Transcriptase ◽  
Reverse Transcription ◽  
Rnase H ◽  
Reverse Transcriptases ◽  
Trna Primer ◽  
Ty1 Retrotransposition ◽  
Retroviral Replication ◽  
Dna Template ◽  
Replication Intermediates

ABSTRACT Ty1 retrotransposition, like retroviral replication, is a complex series of events requiring reverse transcription of an RNA intermediate, RNA-primed minus- and plus-strand DNA synthesis, multiple strand transfers, and precise cleavages of the template and primers by RNase H. In this report, we examine the structure of in vivo Ty1 replication intermediates, specifically with regard to the behavior of reverse transcriptase upon reaching template ends and to the precision with which RNase H might generate these ends. While the expected 3′ termini were always identified, terminal nontemplated bases were also observed at all of the RNA and DNA template ends examined. Nontemplated A residues were most common at all 3′ ends, although C residues were preferentially added to minus-strand termini paused at the 5′ end of capped Ty1 RNA. In addition, we observed that RNase H removal of the tRNA primer and of the polypurine tract was not always precise or efficient. Finally, we noted numerous instances of Ty1 reverse transcriptase transferring from normal Ty1 template ends to various tRNA templates, with continued synthesis to specific modified bases. A similar pattern was obtained for Ty2, indicating that template ends offer unique opportunities for these two related reverse transcriptases to generate errors.

Application of a colorimetric chain-termination assay for characterization of reverse transcriptase from 3′-azido-2′,3′-deoxythymidine-resistant HIV isolates

2002 ◽  
Vol 35 (3) ◽  
pp. 155 ◽  
Author(s):  
Xing-Wu Shao ◽  
Sandra Hjalmarsson ◽  
Johan Lennerstrand ◽  
Bo Svennerholm ◽  
Jonas Blomberg ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Chain Termination
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