scholarly journals Skeletal Muscle Tissue Engineering Using Biological Scaffolds for Repair of Abdominal Wall Defects in a Rabbit Model

10.5772/21544 ◽  
2011 ◽  
Author(s):  
Zuki Abu ◽  
Ayele Taddese ◽  
Noorjahan Banu Mohamed Alitheen ◽  
Noordin Mohamed
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Abdominal Wall ◽  
Muscle Tissue ◽  
Rabbit Model ◽  
Skeletal Muscle Tissue ◽  
Abdominal Wall Defects ◽  
Biological Scaffolds

scholarly journals Engineering of Skeletal Muscle Tissue Using Myoblast-Seeded Bovine Pericardium for Reconstruction of Abdominal Wall Defect in a Rabbit Model

2010 ◽  
Vol 8 ◽  
pp. 9-21
Author(s):  
T. Ayele ◽  
A.B.Z. Zuki ◽  
M.M. Noordin ◽  
B.M.A. Noorjahan
Keyword(s):  
Skeletal Muscle ◽  
Treatment Group ◽  
Abdominal Wall ◽  
Muscle Tissue ◽  
Abdominal Wall Defect ◽  
Rabbit Model ◽  
Bovine Pericardium ◽  
Skeletal Muscle Tissue ◽  
Control Group ◽  
Abdominal Wall Defects

A novel tissue engineered construct was used to engineer skeletal muscle tissue for reconstruction of abdominal wall defects, which is a common challenge to surgeons, due to insufficient autogenous tissue. Myoblasts were isolated from soleus muscle fibers, seeded onto the scaffold and cultivated in vitro for 5 days. Full-thickness abdominal wall defects (3 x 4 cm) were created in 18 male New Zealand white rabbits and randomly divided into two equal groups (n=9 each). The defects of the first group were repaired with myoblast seeded bovine pericardium (treatment group) whereas the second group involved non-seeded bovine pericardium (control group). Three animals were sacrificed at 7, 14, and 30 days post-implantation from each group and the explanted specimens were subjected to macroscopic, light, fluorescence and electron microscopic analysis. In each case, the tissue engineered construct was thicker from deposition of newly formed collagen with neo-vascularisation, than the control group. Most importantly, multinucleated myotubes and myofibers were only detected in the treatment group. Therefore, this study demonstrates that myoblast-seeded bovine pericardium construct can provide a structural replacement for severe and large abdominal wall defects with profound regeneration of skeletal muscle tissues.

scholarly journals Recycled algae-based carbon materials as electroconductive 3D printed skeletal muscle tissue engineering scaffolds

2021 ◽  
Vol 32 (7) ◽  
Author(s):  
Selva Bilge ◽  
Emre Ergene ◽  
Ebru Talak ◽  
Seyda Gokyer ◽  
Yusuf Osman Donar ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Electrical Stimulation ◽  
Muscle Tissue ◽  
Carbonaceous Material ◽  
Hydrothermal Carbonization ◽  
Skeletal Muscle Tissue ◽  
Myotube Formation ◽  
3D Printed

AbstractSkeletal muscle is an electrically and mechanically active tissue that contains highly oriented, densely packed myofibrils. The tissue has self-regeneration capacity upon injury, which is limited in the cases of volumetric muscle loss. Several regenerative therapies have been developed in order to enhance this capacity, as well as to structurally and mechanically support the defect site during regeneration. Among them, biomimetic approaches that recapitulate the native microenvironment of the tissue in terms of parallel-aligned structure and biophysical signals were shown to be effective. In this study, we have developed 3D printed aligned and electrically active scaffolds in which the electrical conductivity was provided by carbonaceous material (CM) derived from algae-based biomass. The synthesis of this conductive and functional CM consisted of eco-friendly synthesis procedure such as pre-carbonization and multi-walled carbon nanotube (MWCNT) catalysis. CM obtained from biomass via hydrothermal carbonization (CM-03) and its ash form (CM-03K) were doped within poly(ɛ-caprolactone) (PCL) matrix and 3D printed to form scaffolds with aligned fibers for structural biomimicry. Scaffolds were seeded with C2C12 mouse myoblasts and subjected to electrical stimulation during the in vitro culture. Enhanced myotube formation was observed in electroactive groups compared to their non-conductive counterparts and it was observed that myotube formation and myotube maturity were significantly increased for CM-03 group after electrical stimulation. The results have therefore showed that the CM obtained from macroalgae biomass is a promising novel source for the production of the electrically conductive scaffolds for skeletal muscle tissue engineering.

Pigmented Silk Nanofibrous Composite for Skeletal Muscle Tissue Engineering

2016 ◽  
Vol 5 (10) ◽  
pp. 1222-1232 ◽  
Author(s):  
Shivaprasad Manchineella ◽  
Greeshma Thrivikraman ◽  
Khadija K. Khanum ◽  
Praveen C. Ramamurthy ◽  
Bikramjit Basu ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Muscle Tissue ◽  
Skeletal Muscle Tissue

scholarly journals Gelatin-genipin-based biomaterials for skeletal muscle tissue engineering

2018 ◽  
Vol 106 (8) ◽  
pp. 2763-2777 ◽  
Author(s):  
Francesca Gattazzo ◽  
Carmelo De Maria ◽  
Alessandro Rimessi ◽  
Silvia Donà ◽  
Paola Braghetta ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Muscle Tissue ◽  
Skeletal Muscle Tissue

Global Evolution of Skeletal Muscle Tissue Engineering: A Scientometric Research

2021 ◽  
Vol 27 (9) ◽  
pp. 497-511
Author(s):  
Xincheng Du ◽  
Fang Xu ◽  
Haowen Qiao ◽  
Wenwen Liu ◽  
Xingdao He ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Muscle Tissue ◽  
Skeletal Muscle Tissue ◽  
Global Evolution

Skeletal muscle tissue engineering

2022 ◽  
pp. 67-80
Author(s):  
Amira Abdalla ◽  
Dathe Benissan-Messan ◽  
Hua Zhu
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Muscle Tissue ◽  
Skeletal Muscle Tissue
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