scholarly journals Use of Radioactive Precursors for Biochemical Characterization the Biosynthesis of Isoprenoids in Intraerythrocytic Stages of Plasmodium falciparum

10.5772/20582 ◽  
2011 ◽  
Author(s):  
Emilia A. ◽  
Gerhard Wunderlich ◽  
Fabiana M. ◽  
Renata Tonhosolo ◽  
Heloisa B. ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Biochemical Characterization

Biochemical characterization of Plasmodium falciparum CTP:phosphoethanolamine cytidylyltransferase shows that only one of the two cytidylyltransferase domains is active

2013 ◽  
Vol 450 (1) ◽  
pp. 159-167 ◽  
Author(s):  
Sweta Maheshwari ◽  
Marina Lavigne ◽  
Alicia Contet ◽  
Blandine Alberge ◽  
Emilie Pihan ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Cellular Localization ◽  
Biochemical Characterization ◽  
De Novo ◽  
Membrane Lipids ◽  
Polyclonal Antibodies ◽  
Homology Modelling ◽  
Site Directed Mutagenesis ◽  
Recombinant Enzymes ◽  
Parasite Life Cycle

The intra-erythrocytic proliferation of the human malaria parasite Plasmodium falciparum requires massive synthesis of PE (phosphatidylethanolamine) that together with phosphatidylcholine constitute the bulk of the malaria membrane lipids. PE is mainly synthesized de novo by the CDP:ethanolamine-dependent Kennedy pathway. We previously showed that inhibition of PE biosynthesis led to parasite death. In the present study we characterized PfECT [P. falciparum CTP:phosphoethanolamine CT (cytidylyltransferase)], which we identified as the rate-limiting step of the PE metabolic pathway in the parasite. The cellular localization and expression of PfECT along the parasite life cycle were studied using polyclonal antibodies. Biochemical analyses showed that the enzyme activity follows Michaelis–Menten kinetics. PfECT is composed of two CT domains separated by a linker region. Activity assays on recombinant enzymes upon site-directed mutagenesis revealed that the N-terminal CT domain was the only catalytically active domain of PfECT. Concordantly, three-dimensional homology modelling of PfECT showed critical amino acid differences between the substrate-binding sites of the two CT domains. PfECT was predicted to fold as an intramolecular dimer suggesting that the inactive C-terminal domain is important for dimer stabilization. Given the absence of PE synthesis in red blood cells, PfECT represents a potential antimalarial target opening the way for a rational conception of bioactive compounds.

scholarly journals Structural and biochemical characterization of a mitochondrial peroxiredoxin from Plasmodium falciparum

2006 ◽  
Vol 61 (4) ◽  
pp. 948-959 ◽  
Author(s):  
Ian W. Boucher ◽  
Paul J. McMillan ◽  
Mads Gabrielsen ◽  
Susan E. Akerman ◽  
James A. Brannigan ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Biochemical Characterization

scholarly journals Structural and biochemical characterization of Plasmodium falciparum Hsp70-x reveals functional versatility of its C-terminal EEVN motif

2018 ◽  
Vol 86 (11) ◽  
pp. 1189-1201 ◽  
Author(s):  
Blessing Mabate ◽  
Tawanda Zininga ◽  
Lebogang Ramatsui ◽  
Stanley Makumire ◽  
Ikechukwu Achilonu ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Biochemical Characterization ◽  
Functional Versatility

scholarly journals Biochemical characterization of Plasmodium falciparum dipeptidyl aminopeptidase 1

2011 ◽  
Vol 175 (1) ◽  
pp. 10-20 ◽  
Author(s):  
Flora Wang ◽  
Priscilla Krai ◽  
Edgar Deu ◽  
Brittney Bibb ◽  
Conni Lauritzen ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Biochemical Characterization ◽  
Dipeptidyl Aminopeptidase

scholarly journals Biochemical characterization of Plasmodium falciparum parasite specific helicase 1 (PfPSH1)

FEBS Open Bio ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 1909-1927
Author(s):  
Manish Chauhan ◽  
Suman Sourabh ◽  
Rahena Yasmin ◽  
Isha Pahuja ◽  
Renu Tuteja
Keyword(s):  
Plasmodium Falciparum ◽  
Biochemical Characterization ◽  
Plasmodium Falciparum Parasite ◽  
Falciparum Parasite

Biochemical Characterization of Plasmodium falciparum Hemozoin

1990 ◽  
Vol 43 (6) ◽  
pp. 584-596 ◽  
Author(s):  
Peter Goldie ◽  
Eugene F. Roth ◽  
Jerome P. Vanderberg ◽  
Joel Oppenheim
Keyword(s):  
Plasmodium Falciparum ◽  
Biochemical Characterization
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