scholarly journals In Vitro Culture Methods of Skin Cells for Optimal Skin Reconstruction by Tissue Engineering

10.5772/20341 ◽  
2011 ◽  
Author(s):  
Moulin VJ ◽  
Mayrand D ◽  
Laforce-Lavoie A ◽  
Larochelle S ◽  
Genest H
Keyword(s):  
Tissue Engineering ◽  
In Vitro Culture ◽  
Culture Methods ◽  
Skin Cells ◽  
Skin Reconstruction

scholarly journals Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods

2020 ◽  
Vol 21 (12) ◽  
Author(s):  
Erma Prihastanti ◽  
ENDAH D. HASTUTI ◽  
SRI W.A. SUEDY
Keyword(s):  
In Vitro Culture ◽  
Shoot Growth ◽  
Short Communication ◽  
Stem Explants ◽  
Citrus Reticulata ◽  
Culture Methods ◽  
Treatment Groups ◽  
Physiological Conditions ◽  
Growth Differences

Abstract. Prihastanti E, Hastuti ED, Suedy SWA. 2020. Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods. Biodiversitas 21: 5845-5849. Several efforts have been made to preserve Citrus reticulata var. Tawangmangu and Citrus reticulata var. Garut as indigenous Indonesian mandarin cultivars, including in vitro tissue culture methods. This study aimed to determine growth differences of the stem explants of C. reticulata var. Tawangmangu and C. reticulata var. Garut,which planted on the same Murashige and Skoog (MS) media. The treatment groups were derived from different explants, grown in 4 separate culture bottles for 35 days at 25°C. The observed parameters included the percentage of explants indicating callus development browned-colored explants, and the contaminated explants. Among C. reticulata var. Tawangmangu explants, 23.53% indicated callus development, 29.42% were browned-colored explants, and 0% indicated contamination. In contrast, among the C. reticulata var. Garut explants, 0% indicated callus development, 7.14% brown-colored, and 7.14% indicated contamination. The stems explants from C. reticulata var. Tawangmangu showed a tendency to develop calluses, but the explants of C. reticulata var. Garut was able to support the growth of shoots. C. reticulata var. Tawangmangu and Garut mandarin stem explants showed differences of shoot growth because physiological conditions varied according to the variety.

Biodegradable parallel and porous HSPG/collagen scaffolds for the in vitro culture of NSCs for the spinal cord tissue engineering

2011 ◽  
Vol 19 (2) ◽  
pp. 173-180 ◽  
Author(s):  
Yansong Wang ◽  
Changwei Zhou ◽  
Meng Yao ◽  
Ying Li ◽  
Yugang Liu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Tissue Engineering ◽  
In Vitro Culture ◽  
Spinal Cord Tissue ◽  
Collagen Scaffolds

In vitro culture methods in sorghum with shoot tip as the explant material

2006 ◽  
Vol 25 (3) ◽  
pp. 174-182 ◽  
Author(s):  
N Kishore Sai ◽  
KBRS Visarada ◽  
Y. Aravinda Lakshmi ◽  
E. Pashupatinath ◽  
S. V. Rao ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Shoot Tip ◽  
Culture Methods

scholarly journals BIOTECNOLOGIA DE CÉLULAS TRONCO MESENQUIMAIS: PRINCIPAIS APLICAÇÕES EM MEDICINA VETERINÁRIA E PERSPECTIVAS GERAIS

2017 ◽  
Vol 13 (Especial 2) ◽  
pp. 83-89
Author(s):  
Elaine Cristina Galhardo ◽  
Ariane Dantas ◽  
Aline Sousa Camargos ◽  
Fernanda da Cruz Landim
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Veterinary Medicine ◽  
In Vitro Culture ◽  
Adult Tissues ◽  
Inflammatory Processes ◽  
Specific Tissue

Mesenchymal Stem Cells (MSCs) are isolated from adult tissues and presents ability to originate identical cells or differentiate into specific tissue cells. Studies about in vitro culture and in vivo application of MSCs have pointed the potential to be explored in regenerative treatments and modulation of inflammatory processes, as well as the improvement of tissue engineering techniques and the development of biomaterials. Veterinary Medicine has presented, in several species, satisfactory results coming from the regenerative potential and paracrine activity characteristic of the MSCs. This review aimed, briefly showing promising results of the experimental MSCs application in Veterinary Medicine, its current biotechnological overview and general prospects.

Regulation of meiosis in the foetal mouse gonad

Development ◽  
1982 ◽  
Vol 68 (1) ◽  
pp. 59-67
Author(s):  
Clive W. Evans ◽  
Diana I. Robb ◽  
Fiona Tuckett ◽  
Susan Challoner
Keyword(s):  
In Vitro Culture ◽  
Cross Section ◽  
Germ Cells ◽  
Sertoli Cells ◽  
Inhibitory Effects ◽  
Culture Methods ◽  
Male And Female ◽  
Mixed Age ◽  
Gonadal Structure

In vitro culture of male and female gonads was found to have significant effects on gonadal structure and development. Culture resulted in a reduction of testicular cord diameter and a reduction in the number of Sertoli cells lining each cord in cross section. In the female, culture increased the percentage of pyknotic oocytes and fewer germ cells per unit of ovary volume reached diplotene. Mixed sex co-culture using different culture methods showed that day 14 p.c. testes inhibited meiosis in day 14 p.c. ovaries when the cultures were continued until the equivalent of day 21 p.c. Day 15 p.c. and mixed age co-cultures of mixed sex provided more equivocal data since meiosis was inhibited in some preparations but not in others. The possibility is suggested that prophase I may proceed irrevocably to diplotene after about day 15 p.c. and thus the inhibitory effects of foetal testes may be a function of female gonadal age. No evidence was found to support the hypothesis that mixed sex co-culture may stimulate meiosis precociously in foetal testes.

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