scholarly journals Dictyostelium discoideum: A Model System to Study LRRK2-Mediated Parkinson Disease

10.5772/20207 ◽  
2012 ◽  
Author(s):  
Arjan Kortholt ◽  
Bernd Gilsbach ◽  
Peter J.M. van Haastert
Keyword(s):  
Parkinson Disease ◽  
Dictyostelium Discoideum ◽  
Model System

scholarly journals Microbe Profile: Dictyostelium discoideum: model system for development, chemotaxis and biomedical research

Microbiology ◽  
2021 ◽  
Author(s):  
Catherine J. Pears ◽  
Julian D. Gross
Keyword(s):  
Pattern Formation ◽  
Cell Motility ◽  
Dictyostelium Discoideum ◽  
Biomedical Research ◽  
Model System ◽  
Developmental Pattern ◽  
Social Amoeba ◽  
The Social ◽  
Host Pathogen ◽  
Soil Amoeba

The social amoeba Dictyostelium discoideum is a versatile organism that is unusual in alternating between single-celled and multi-celled forms. It possesses highly-developed systems for cell motility and chemotaxis, phagocytosis, and developmental pattern formation. As a soil amoeba growing on microorganisms, it is exposed to many potential pathogens; it thus provides fruitful ways of investigating host-pathogen interactions and is emerging as an influential model for biomedical research.

scholarly journals Teaching a biology laboratory course using Dictyostelium

2019 ◽  
Vol 63 (8-9-10) ◽  
pp. 551-561
Author(s):  
David A. Knecht ◽  
Kate M. Cooper ◽  
Jonathan E. Moore
Keyword(s):  
Dictyostelium Discoideum ◽  
Cell Biology ◽  
Room Temperature ◽  
Model System ◽  
Educational Settings ◽  
Biology Teaching ◽  
Laboratory Course ◽  
Cellular Processes ◽  
Laboratory Courses ◽  
Biology Laboratory

The Dictyostelium discoideum model system is a powerful tool for undergraduate cell biology teaching laboratories. The cells are biologically safe, grow at room temperature and it is easy to experimentally induce, observe, and perturb a breadth of cellular processes making the system amenable to many teaching lab situations and goals. Here we outline the advantages of Dictyostelium, discuss laboratory courses we teach in three very different educational settings, and provide tips for both the novice and experienced Dictyostelium researcher. With this article and the extensive sets of protocols and tools referenced here, implementing these labs, or parts of them, will be relatively straightforward for any instructor.

scholarly journals The FtsZ Homolog, FszB, Inhibits Mitochondrial Dynamics in Dictyostelium discoideum

Cells ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 64 ◽  
Author(s):  
Ericka Vogel ◽  
Pristine Bay Pittman ◽  
Kari Naylor
Keyword(s):  
Dictyostelium Discoideum ◽  
Mitochondrial Dynamics ◽  
Model System ◽  
Membrane Remodeling ◽  
Positive Role ◽  
Actual Mechanism

Dictyostelium discoideum is a well-established mitochondrial model system for both disease and dynamics, yet we still do not understand the actual mechanism of mitochondrial dynamics in this system. The FtsZ proteins are known to mediate membrane remodeling events such as cytokinesis in bacteria and fission of chloroplasts; D. discoideum has two FtsZ proteins, FszA and FszB. To determine the role of these proteins in mitochondrial dynamics we overexpressed FszB-GFP and determined its effect on fission, fusion, and motility in the presence of intact and disrupted cytoskeletal filaments. Here we show that overexpression of FszB-GFP decreases mitochondrial dynamics and suggest that actin may play a positive role driving fission in the context of excessive inhibition by overexpressed FszB-GFP.

scholarly journals The Dictyostelium discoideum model system

2019 ◽  
Vol 63 (8-9-10) ◽  
pp. 317-320 ◽  
Author(s):  
Ricardo Escalante ◽  
Elena Cardenal-Muñoz
Keyword(s):  
Cell Motility ◽  
Dictyostelium Discoideum ◽  
Cell Biology ◽  
Model Organism ◽  
Opportunity To Learn ◽  
Model System ◽  
Special Issue ◽  
Complete Understanding ◽  
Single Model ◽  
Present Collection

When we set out to organize this Special Issue, we faced the difficult task of gathering together a large variety of topics with the unique commonality of having been studied in a single model organism, Dictyostelium discoideum. This apparent setback turned into a wonderful opportunity to learn about an organism as a whole, which provides a more complete understanding of life processes, their natural meaning and their changes during evolution. From studies dedicated almost exclusively to cell motility, differentiation and patterning, the versatility of D. discoideum has allowed in recent years the expansion of our knowledge to other areas, including cell biology and many others related to human diseases. The present collection of papers can be considered as a journey throughout the mechanisms of life, where D. discoideum acts as a very special tourist guide.

Dictyostelium discoideum: A Model System to Study Autophagy Mediated Life Extension

2016 ◽  
pp. 35-55
Author(s):  
Punita Jain ◽  
Priyanka Sharma ◽  
Anju Shrivastava ◽  
Shweta Saran
Keyword(s):  
Dictyostelium Discoideum ◽  
Model System ◽  
Life Extension

scholarly journals Dictyostelium discoideum as a pharmacological model system to study the mechanisms of medicinal drugs and natural products

2019 ◽  
Vol 63 (8-9-10) ◽  
pp. 541-550 ◽  
Author(s):  
Judith Schaf ◽  
Joseph Damstra-Oddy ◽  
Robin S.B. Williams
Keyword(s):  
Natural Products ◽  
Dictyostelium Discoideum ◽  
Cellular Level ◽  
Model System ◽  
Genetic Redundancy ◽  
Therapeutic Drugs ◽  
Pharmacologically Active ◽  
Medicinal Drugs ◽  
Pharmacological Model ◽  
Active Natural

Developing novel compounds for the treatment of diseases remains one of the highest priorities in biomedical research, where it is critical to identify their targets and how they work at a cellular level. Most studies in this area employ mammalian models, since rodents or non-human primates are seen as a good approximation for humans. However, using mammalian models can be problematic for a range of reasons, including high genetic redundancy and the essential role for many proteins in development. More importantly, it is very difficult to identify how compounds function at a cellular or molecular level in these models without a previously suggested mechanism or target. So how can we identify targets of medicinal compounds? In this review we outline the use of an innovative and tractable model system, Dictyostelium discoideum, to provide useful insight to the cellular and molecular functions of both therapeutic drugs and pharmacologically active natural products. We outline the advantages of using this model, and then provide a range of exemplar studies using D. discoideum in pharmacological research to demonstrate breakthroughs in understanding the action and effects of compounds, and the subsequent translational of these advances to mammalian models leading to potential improvements in societal health.

scholarly journals Proteomic characterization of the Mycobacterium marinum-containing vacuole in Dictyostelium discoideum

2019 ◽  
Author(s):  
Aurélie Guého ◽  
Cristina Bosmani ◽  
Thierry Soldati
Keyword(s):  
Dictyostelium Discoideum ◽  
Model System ◽  
Mycobacterium Marinum ◽  
Close Relative ◽  
Phagosome Maturation ◽  
Fish Pathogen ◽  
Free Living ◽  
Free Living Amoeba ◽  
Professional Phagocyte

ABSTRACTMycobacterium tuberculosis, the causative agent of tuberculosis, is able to manipulate the phagosome compartment where it resides in order to establish a permissive replicative compartment called the Mycobacterium-containing vacuole (MCV). Mycobacterium marinum, a fish pathogen and a close relative of the tuberculosis group, is also able to infect the free-living amoeba and professional phagocyte Dictyostelium discoideum and to manipulate its phagosome maturation. By using this host/pathogen model system, we have established an innovative procedure to isolate MCVs. This procedure allowed us to isolate M. marinum-MCV at 1, 3 and 6 hours post infection to study the early M. marinum-MCV proteome. By using isobaric labelling and mass spectrometry, we quantitatively compared the proteomic composition of those MCVs isolated at different stages of the early infection phase to understand how M. marinum impacts on this compartment to divert it from the normal phagosomal pathway. Furthermore, we also compare the manipulated compartment M. marinum-MCV to non- or less manipulated compartments containing different mycobacteria strains: the non-pathogenic M. smegmatis, the avirulent M. marinum-L1D or the attenuated M. marinum-RD1.

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