scholarly journals Soybean: Plant Manipulation to Agrobacterium Mediated Transformation

10.5772/19881 ◽  
2011 ◽  
Author(s):  
Muhammad Zia
Keyword(s):  
Soybean Plant ◽  
Agrobacterium Mediated Transformation

Agrobacterium-Mediated Transformation Of Plant Cells

2018 ◽  
Author(s):  
Christine Kronfoth ◽  
◽  
Peter Grayson ◽  
Keyword(s):  
Plant Cells ◽  
Agrobacterium Mediated Transformation

Comparison of Nodular Efficiencies Between Partridge Pea and Soybean Using Acetylene Reduction

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 449f-450
Author(s):  
Lisa M. Barry ◽  
Michael N. Dana
Keyword(s):  
Growth Rate ◽  
Plant Size ◽  
Silica Sand ◽  
Soybean Plant ◽  
Fibrous Root ◽  
Constant Growth Rate ◽  
Soil Microbial ◽  
Soybean Plants ◽  
Nurse Crops ◽  
Partridge Pea

Legumes are grown as nurse crops in agriculture because they increase soil microbial life and productivity. Native legumes have potential in ecological restoration to mimic the benefits found in agriculture plus they enhance the restored ecosystem. This study was initiated to compare the growth rates, nodulation characteristics, and nitrogen fixation rates of a native versus a non-native legume. The two legumes were partridge pea (Cassia fasciculata); a native, wild, annual legume and soybean (Glycine max `Century Yellow); a domesticated, agricultural, annual legume native to Asia. Plants were grown for 11 weeks in pots containing silica sand and received a nitrogen-free Hoagland's nutrient solution. Beginning at week 12, plants were harvested weekly for four consecutive weeks. Nodulated root systems were exposed to acetylene gas and the resulting ethylene amounts were measured. The two legumes exhibited significant differences in nodule size and shape and plant growth rate. In soybean, nodules were large, spherical, and clustered around the taproot while in partridge pea, nodules were small, irregularly shaped, and spread throughout the fibrous root system. Soybean plants had a significantly faster growth rate at the onset of the experiment but partridge pea maintained a constant growth rate and eventually exceeded soybean plant size. In spite of these observed differences, partridge pea and soybean plants were equally efficient at reducing acetylene to ethylene. These results indicate partridge pea has the potential to produce as much nitrogen in the field as soybean. Native legumes such as partridge pea deserve further research to explore their use as nurse crops in agricultural or restoration regimes.

Putative Alleles for Increased Yield from Soybean Plant Introductions

Crop Science ◽  
2004 ◽  
Vol 44 (3) ◽  
pp. 784 ◽  
Author(s):  
E. A. Kabelka ◽  
B. W. Diers ◽  
W. R. Fehr ◽  
A. R. LeRoy ◽  
I. C. Baianu ◽  
...  
Keyword(s):  
Soybean Plant ◽  
Plant Introductions

scholarly journals Machine Learning Techniques to Predict Soybean Plant Density Using UAV and Satellite-Based Remote Sensing

2021 ◽  
Vol 13 (13) ◽  
pp. 2548
Author(s):  
Luthfan Nur Habibi ◽  
Tomoya Watanabe ◽  
Tsutomu Matsui ◽  
Takashi S. T. Tanaka
Keyword(s):  
Spatial Variability ◽  
Prediction Accuracy ◽  
Regression Models ◽  
Plant Density ◽  
Density Measurement ◽  
Critical Factor ◽  
Detection Algorithm ◽  
Machine Learning Techniques ◽  
Soybean Plant ◽  
Climate Data

The plant density of soybean is a critical factor affecting plant canopy structure and yield. Predicting the spatial variability of plant density would be valuable for improving agronomic practices. The objective of this study was to develop a model for plant density measurement using several data sets with different spatial resolutions, including unmanned aerial vehicle (UAV) imagery, PlanetScope satellite imagery, and climate data. The model establishment process includes (1) performing the high-throughput measurement of actual plant density from UAV imagery with the You Only Look Once version 3 (YOLOv3) object detection algorithm, which was further treated as a response variable of the estimation models in the next step, and (2) developing regression models to estimate plant density in the extended areas using various combinations of predictors derived from PlanetScope imagery and climate data. Our results showed that the YOLOv3 model can accurately measure actual soybean plant density from UAV imagery data with a root mean square error (RMSE) value of 0.96 plants m−2. Furthermore, the two regression models, partial least squares and random forest (RF), successfully expanded the plant density prediction areas with RMSE values ranging from 1.78 to 3.67 plant m−2. Model improvement was conducted using the variable importance feature in RF, which improved prediction accuracy with an RMSE value of 1.72 plant m−2. These results demonstrated that the established model had an acceptable prediction accuracy for estimating plant density. Although the model could not often evaluate the within-field spatial variability of soybean plant density, the predicted values were sufficient for informing the field-specific status.

scholarly journals Citrus Cell Suspension Culture Establishment, Maintenance, Efficient Transformation and Regeneration to Complete Transgenic Plant

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 664
Author(s):  
M. Moniruzzaman ◽  
Yun Zhong ◽  
Zhifeng Huang ◽  
Huaxue Yan ◽  
Lv Yuanda ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Culture System ◽  
Transformation Rate ◽  
Suspension Cell Culture ◽  
Citrus Reticulata ◽  
Agrobacterium Mediated Transformation ◽  
Solid Media

Agrobacterium-mediated transformation of epicotyl segment has been used in Citrus transgenic studies. The approach suffers, however, from limitations such as occasionally seed unavailability, the low transformation efficiency of juvenile tissues and the high frequency of chimeric plants. Therefore, a suspension cell culture system was established and used to generate transgenic plants in this study to overcome the shortcomings. The embryonic calli were successfully developed from undeveloped ovules of the three cultivars used in this study, “Sweet orange”-Egyptian cultivar (Citrus sinensis), “Shatangju” (Citrus reticulata) and “W. Murcott” (Citrus reticulata), on three different solid media. Effects of media, genotypes and ages of ovules on the induction of embryonic calli were also investigated. The result showed that the ovules’ age interferes with the callus production more significantly than media and genotypes. The 8 to 10 week-old ovules were found to be the best materials. A cell suspension culture system was established in an H+H liquid medium. Transgenic plants were obtained from Agrobacterium-mediated transformation of cell suspension as long as eight weeks subculture intervals. A high transformation rate (~35%) was achieved by using our systems, confirming BASTA selection and later on by PCR confirmation. The results demonstrated that transformation of cell suspension should be more useful for the generation of non-chimeric transgenic Citrus plants. It was also shown that our cell suspension culture procedure was efficient in maintaining the vigor and regeneration potential of the cells.

scholarly journals Action of growth regulators on flowering and pod production of soybean cultivar Davis

1981 ◽  
Vol 38 (1) ◽  
pp. 99-112
Author(s):  
Paulo R.C. Castro ◽  
Roberto S. Moraes
Keyword(s):  
Glycine Max ◽  
Gibberellic Acid ◽  
Growth Regulators ◽  
Lower Number ◽  
Soybean Cultivar ◽  
Soybean Plant ◽  
Trimethylammonium Chloride ◽  
Triiodobenzoic Acid ◽  
Soybean Plants

This research deals with the effects of growth regulators on flowering and pod formation in soybean plant (Glycine max cv. Davis). Under greenhouse conditions, soybean plants were sprayed with 2,3,5-triiodobenzoic acid (TIBA) 20 ppm, Agrostemmin (1g/10 ml/3 l) gibberellic acid (GA) 100 ppm, and (2-chloroethyl) trimethylammonium chloride (CCC) 2,000 ppm. Application of TIBA increased number of flowers. 'Davis' soybean treated with CCC and TIBA presented a tendency to produce a lower number of pods.

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