scholarly journals Application of the Ultramicro Forward-Mutation Assay to the Monitoring of Indoor and Outdoor Air Mutagenicity-Examples of Chengdu City and Tokyo

10.5772/19237 ◽  
2011 ◽  
Author(s):  
Yukihiko Takagi ◽  
Daisuke Nakajima ◽  
Sun Chengjun ◽  
Sumio Goto
Keyword(s):  
Outdoor Air ◽  
Mutation Assay ◽  
Forward Mutation ◽  
Indoor And Outdoor

Indoor and outdoor air quality monitoring in hemp house — Case study

2011 ◽  
Vol 6 (3) ◽  
pp. 63-72 ◽  
Author(s):  
Jarmila Rimbalová ◽  
Silvia Vilčeková ◽  
Adriana Eštoková
Keyword(s):  
Air Quality ◽  
Quality Monitoring ◽  
Air Quality Monitoring ◽  
Outdoor Air ◽  
Outdoor Air Quality ◽  
Indoor And Outdoor

scholarly journals Indoor and Outdoor Air Quality for Sustainable Life: A Case Study of Rural and Urban Settlements in Poor Neighbourhoods in Kenya

2021 ◽  
Vol 13 (4) ◽  
pp. 2417
Author(s):  
Anne Wambui Mutahi ◽  
Laura Borgese ◽  
Claudio Marchesi ◽  
Michael J. Gatari ◽  
Laura E. Depero
Keyword(s):  
Air Quality ◽  
Elemental Composition ◽  
Black Carbon ◽  
Informal Settlement ◽  
Heavy Traffic ◽  
World Health ◽  
Rural Settlements ◽  
Outdoor Air ◽  
Outdoor Air Quality ◽  
Indoor And Outdoor

This paper reports on the indoor and outdoor air quality in informal urban and rural settlements in Kenya. The study is motivated by the need to improve consciousness and to understand the harmful health effects of air quality to vulnerable people, especially in poor communities. Ng’ando urban informal settlement and Leshau Pondo rural village in Kenya are selected as representative poor neighborhoods where unclean energy sources are used indoor for cooking, lighting and heating. Filter based sampling for gravimetrical, elemental composition and black carbon (BC) analysis of particulate matter with an aerodynamic diameter less than 2.5 µm (PM2.5) is performed. findings from Ng’ando and Leshau Pondo showed levels exceeding the limit suggested by the world health organization (WHO), with rare exceptions. Significantly higher levels of PM2.5 and black carbon are observed in indoors than outdoor samples, with a differences in the orders of magnitudes and up to 1000 µg/m3 for PM2.5 in rural settlements. The elemental composition reveals the presence of potentially toxic elements, in addition to characterization, emission sources were also identified. Levels of Pb exceeding the WHO limit are found in the majority of samples collected in the urban locations near major roads with heavy traffic. Our results demonstrate that most of the households live in deplorable air quality conditions for more than 12 h a day and women and children are more affected. Air quality condition is much worse in rural settlements where wood and kerosene are the only available fuels for their energy needs.

A novel bacterial reversion and forward mutation assay based on green fluorescent protein

1998 ◽  
Vol 414 (1-3) ◽  
pp. 95-105 ◽  
Author(s):  
Neal F Cariello ◽  
Sabrina Narayanan ◽  
Puntipa Kwanyuen ◽  
Heidi Muth ◽  
Warren M Casey
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent ◽  
Mutation Assay ◽  
Forward Mutation

scholarly journals The TREX2 3′→ 5′ Exonuclease Physically Interacts with DNA Polymerase δ and Increases Its Accuracy

2002 ◽  
Vol 2 ◽  
pp. 275-281 ◽  
Author(s):  
Igor V. Shevelev ◽  
Kristijan Ramadan ◽  
Ulrich Hubscher
Keyword(s):  
Dna Replication ◽  
Dna Polymerase ◽  
Error Rates ◽  
High Fidelity ◽  
Replication Machinery ◽  
Dna Polymerase Δ ◽  
Pol I ◽  
Calf Thymus ◽  
Mutation Assay ◽  
Forward Mutation

Proofreading function by the 3′→ 5′ exonuclease of DNA polymerase δ (pol δ) is consistent with the observation that deficiency of the associated exonuclease can lead to a strong mutation phenotype, high error rates during DNA replication, and ultimately cancer. We have isolated pol δdfrom isotonic (pol δi) and detergent (pol δd) calf thymus extracts. Pol δdhad a 20-fold higher ratio of exonuclease to DNA polymerase than pol δi. This was due to the physical association of the TREX2 exonuclease to pol δd, which was missing from pol δi. Pol δdwas fivefold more accurate than pol δiunder error-prone conditions (1 μM dGTP and 20 dATP, dCTP, and dTTP) in a M13mp2 DNA forward mutation assay, and fourfold more accurate in an M13mp2T90 reversion assay. Under error-free conditions (20 μM each of the four dNTPs), however, both polymerases showed equal fidelity. Our data suggested that autonomous 3′→ 5′ exonucleases, such as TREX2, through its association with pol I can guarantee high fidelity under difficult conditions in the cell (e.g., imbalance of dNTPs) and can add to the accuracy of the DNA replication machinery, thus preventing mutagenesis.

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