scholarly journals Oral Bacterial Adhesion and Biocompatibility of Silver-Amorphous Carbon Films: A Surface Modification for Dental Implants

10.5772/18298 ◽  
2011 ◽  
Author(s):  
Argelia Almaguer-Flore ◽  
Sandra E. ◽  
Rene Olivares-Navarrete
Keyword(s):  
Surface Modification ◽  
Dental Implants ◽  
Amorphous Carbon ◽  
Bacterial Adhesion ◽  
Carbon Films ◽  
Amorphous Carbon Films

scholarly journals Surface modification and the friction coefficient of tetrahedral amorphous carbon films bombarded by energetic N ion

2011 ◽  
Vol 60 (6) ◽  
pp. 066804
Author(s):  
Han Liang ◽  
Chen Xian ◽  
Yang Li ◽  
Wang Yan-Wu ◽  
Wang Xiao-Yan ◽  
...  
Keyword(s):  
Surface Modification ◽  
Friction Coefficient ◽  
Amorphous Carbon ◽  
Carbon Films ◽  
Amorphous Carbon Films ◽  
Tetrahedral Amorphous Carbon

The effects of amorphous carbon films deposited on polyethylene terephthalate on bacterial adhesion

Biomaterials ◽  
2004 ◽  
Vol 25 (16) ◽  
pp. 3163-3170 ◽  
Author(s):  
J. Wang ◽  
N. Huang ◽  
P. Yang ◽  
Y.X. Leng ◽  
H. Sun ◽  
...  
Keyword(s):  
Polyethylene Terephthalate ◽  
Amorphous Carbon ◽  
Bacterial Adhesion ◽  
Carbon Films ◽  
Amorphous Carbon Films

In situ surface modification and growth of ultrasmooth amorphous carbon films by direct carbon ion-beam deposition

1999 ◽  
Vol 14 (6) ◽  
pp. 2668-2673 ◽  
Author(s):  
M. H. Sohn ◽  
S. I. Kim
Keyword(s):  
Surface Roughness ◽  
Surface Modification ◽  
Amorphous Carbon ◽  
Ion Beam ◽  
Carbon Film ◽  
Carbon Films ◽  
Wear Testing ◽  
Amorphous Carbon Films ◽  
Carbon Ion

Very thin (<,100 nm) amorphous carbon films were grown on silicon substrates by unfiltered and filtered direct carbon ion beams. In situ surface modification was performed using C- energies in the range of 300–500 eV prior to the growth of the film. By lowering the energy of the C− beam to 150 eV, an amorphous carbon film was continuously grown after the surface modification. High-resolution electron microscopy showed that the film/substrate interface was damaged by 400 and 500 eV C− beams. The carbon composition profile at the interface investigated by electron energy-loss spectroscopy illustrated that the 500 eV C− beam generated a 30 nm thick carbon/silicon mixing layer at the interface. The damage and mixing layers were not observed at 300 eV modification. Wear testing found that strong adhesion occurred in samples modified at 400 and 500 eV. However, at 300 eV, modified samples exhibited delamination failure, which indicated inferior adhesion of the films. Surface roughness evolution of 30, 60, and 90 nm thick films was investigated by atomic force microscopy. The film surface roughness decrease as a function of film thickness was much faster when the films were grown by the filtered C− beam.

Biocompatibility and Anti-microbial Properties of Silver Modified Amorphous Carbon Films

2009 ◽  
Vol 1244 ◽  
Author(s):  
Argelia Almaguer-Flores ◽  
René Olivares-Navarrete ◽  
Laurie A. Ximénez-Fyvie ◽  
Oscar García-Zarco ◽  
Sandra E. Rodil
Keyword(s):  
Silver Nanoparticles ◽  
Amorphous Carbon ◽  
Bacterial Adhesion ◽  
Specific Activity ◽  
Bacterial Species ◽  
Antimicrobial Properties ◽  
Carbon Films ◽  
Oral Bacteria ◽  
Amorphous Carbon Films ◽  
Microbial Properties

ABSTRACTInfection due microbes on implant surfaces has a strong influence on healing and long term viability of dental implants. The prevention and control of biofilms can be achieved by reducing the bacterial adhesion on the surface. The coating of medical devices with silver, or the addition of silver nanoparticles, are two possible ways to prevent device-associated infections. On the other hand, amorphous carbon films, in its different forms and compositions, have been studied as beneficial surface modification for implant materials. However, the bacterial adhesion on these films by oral bacteria in comparison to standard surfaces (Ti and SS) has been seen to be relatively high. In the oral cavity, the microbial ecology is complex and consists of hundreds of bacterial species, and therefore it is recommendable to study bacteria adhesion using various strains. In this work, we tested the biocompatibility and the anti-microbial properties of amorphous carbon films with the addition of silver nanoparticles. The a-C:Ag films were deposited by co-sputtering in an Argon plasma using a target made of graphite with a small piece of pure silver. Biocompatibility tests were performed using osteoblast-like cells (MG63) and included: cell proliferation, alkaline phosphatase specific activity and OPG. The bacterial adhesion test was evaluated after 1, 3 and 7 days of incubation. We used nine oral bacteria strains: Aggregatibacter actinomycetemcomitans serotype b, Actinomyces israelii, Campylobacter rectus, Eikenella corrodens, Fusobacterium nucleatum ss nucleatum, Parvimonas micra, Porphyromonas gingivalis, Prevotella intermedia and Streptococcus sanguinis. The effect of including silver in the a-C films was studied by X-ray Diffraction, Energy Dispersive spectroscopy, Scanning Electron Microscopy. The results showed that the films had silver nanoparticles (40-60 nm) uniformly distributed in the carbon matrix. The silver was crystalline with a maximum content of around 6 at%. The biological tests showed that a-C:Ag films had good biocompatibility properties, allowing the osteoblast to proliferate and produced osteogenic local factors. Concerning the antimicrobial properties of the a-C:Ag films, we did not observe an effect of the silver particles on bacterial adherence after 1 and 3 days of incubation; however, a significant reduction was observed after 7 days, compared to the a-C, Ti films or the bare SS substrate, suggesting that silver nanoparticles have a time-dependent antimicrobial effect.

Oral bacterial adhesion on amorphous carbon films

2009 ◽  
Vol 18 (9) ◽  
pp. 1179-1185 ◽  
Author(s):  
A. Almaguer-Flores ◽  
R. Olivares-Navarrete ◽  
A. Lechuga-Bernal ◽  
L.A. Ximénez-Fyvie ◽  
S.E. Rodil
Keyword(s):  
Amorphous Carbon ◽  
Bacterial Adhesion ◽  
Carbon Films ◽  
Amorphous Carbon Films

Scanning Tunneling Microscopy of Amorphous Carbon Films

1990 ◽  
Vol 48 (1) ◽  
pp. 318-319
Author(s):  
Mircea Fotino ◽  
D.C. Parks
Keyword(s):  
Scanning Tunneling Microscopy ◽  
Amorphous Carbon ◽  
Carbon Films ◽  
Atomic Scale ◽  
Scanning Tunneling ◽  
Tunneling Microscopy ◽  
Amorphous Carbon Films ◽  
Image Optimization ◽  
Step Procedure ◽  
Stm Images

In the last few years scanning tunneling microscopy (STM) has made it possible and easily accessible to visualize surfaces of conducting specimens at the atomic scale. Such performance allows the detailed characterization of surface morphology in an increasing spectrum of applications in a wide variety of fields. Because the basic imaging process in STM differs fundamentally from its equivalent in other well-established microscopies, good understanding of the imaging mechanism in STM enables one to grasp the correct information content in STM images. It thus appears appropriate to explore by STM the structure of amorphous carbon films because they are used in many applications, in particular in the investigation of delicate biological specimens that may be altered through the preparation procedures.All STM images in the present study were obtained with the commercial instrument Nanoscope II (Digital Instruments, Inc., Santa Barbara, California). Since the importance of the scanning tip for image optimization and artifact reduction cannot be sufficiently emphasized, as stressed by early analyses of STM image formation, great attention has been directed toward adopting the most satisfactory tip geometry. The tips used here consisted either of mechanically sheared Pt/Ir wire (90:10, 0.010" diameter) or of etched W wire (0.030" diameter). The latter were eventually preferred after a two-step procedure for etching in NaOH was found to produce routinely tips with one or more short whiskers that are essentially rigid, uniform and sharp (Fig. 1) . Under these circumstances, atomic-resolution images of cleaved highly-ordered pyro-lytic graphite (HOPG) were reproducibly and readily attained as a standard criterion for easily recognizable and satisfactory performance (Fig. 2).

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