scholarly journals Artificial Acellular Feeder Layer: An Advanced Engineered Extracellular Matrix for Stem Cell Culture

10.5772/15348 ◽  
2011 ◽  
Author(s):  
Amranul Haque ◽  
Masato Nagaoka ◽  
Xiao-Shan Yue ◽  
Stephen A. ◽  
Toshihiro Akaike
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Feeder Layer ◽  
Stem Cell Culture

scholarly journals Stem cell culture on polyvinyl alcohol hydrogels having different elasticity and immobilized with ECM-derived oligopeptides

2017 ◽  
Vol 37 (7) ◽  
pp. 647-660 ◽  
Author(s):  
Saradaprasan Muduli ◽  
Li-Hua Chen ◽  
Meng-Pei Li ◽  
Zhao-wen Heish ◽  
Cheng-Hui Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Cell Fate ◽  
Es Cells ◽  
Embryonic Stem ◽  
Poly Vinyl Alcohol ◽  
Hematopoietic Stem ◽  
Stem Cell Culture

Abstract The physical characteristics of cell culture materials, such as their elasticity, affect stem cell fate with respect to cell proliferation and differentiation. We systematically investigated the morphologies and characteristics of several stem cell types, including human amniotic-derived stem cells, human hematopoietic stem cells, human induced pluripotent stem (iPS) cells, and embryonic stem (ES) cells on poly(vinyl alcohol) (PVA) hydrogels immobilized with and without extracellular matrix-derived oligopeptide. Human ES cells did not adhere well to soft PVA hydrogels immobilized with oligovitronectin, whereas they did adhere well to PVA hydrogel dishes with elasticities greater than 15 kPa. These results indicate that biomaterials such as PVA hydrogels should be designed to possess minimum elasticity to facilitate human ES cell attachment. PVA hydrogels immobilized with and without extracellular matrix-derived oligopeptides are excellent candidates of cell culture biomaterials for investigations into how cell culture biomaterial elasticity affects stem cell culture and differentiation.

Electrospinning adipose tissue-derived extracellular matrix for adipose stem cell culture

2012 ◽  
Vol 100A (7) ◽  
pp. 1716-1724 ◽  
Author(s):  
Michael P. Francis ◽  
Patrick C. Sachs ◽  
Parthasarathy A. Madurantakam ◽  
Scott A. Sell ◽  
Lynne W. Elmore ◽  
...  
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Adipose Stem Cell ◽  
Stem Cell Culture

Extracellular Matrix Isolated From Foreskin Fibroblasts Supports Long-Term Xeno-Free Human Embryonic Stem Cell Culture

2010 ◽  
Vol 19 (4) ◽  
pp. 547-556 ◽  
Author(s):  
Guoliang Meng ◽  
Shiying Liu ◽  
Xiangyun Li ◽  
Roman Krawetz ◽  
Derrick E. Rancourt
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Culture

Neural Stem Cell Culture: Neurosphere generation, microscopical analysis and cryopreservation

2006 ◽  
Author(s):  
Laura Pacey ◽  
Shelley Stead ◽  
Jacqueline Gleave ◽  
Kasia Tomczyk ◽  
Laurie Doering
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Stem Cell Culture ◽  
Microscopical Analysis

scholarly journals Nanofibrous Matrix of Defined Composition Sustains Human Induced Pluripotent Stem Cell Culture

2021 ◽  
Vol 4 (4) ◽  
pp. 3035-3040
Author(s):  
Sara Borrego-González ◽  
Berta de la Cerda ◽  
Francisco J. Díaz-Corrales ◽  
Aránzazu Díaz-Cuenca
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Stem Cell Culture ◽  
Induced Pluripotent

Feeder-Dependent/Independent Mouse Embryonic Stem Cell Culture Protocol

2021 ◽  
Author(s):  
Hatice Burcu Şişli ◽  
Selinay Şenkal ◽  
Derya Sağraç ◽  
Taha Bartu Hayal ◽  
Ayşegül Doğan
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Stem Cell Culture ◽  
Cell Culture Protocol ◽  
Culture Protocol
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