A Candidate Gene in an Ecological Model Species: Phosphoglucose Isomerase (Pgi) in the Glanville Fritillary Butterfly (Melitaea cinxia)

2017 ◽  
Vol 54 (1-4) ◽  
pp. 259-273 ◽  
Author(s):  
Kristjan Niitepõld ◽  
Marjo Saastamoinen
Keyword(s):  
Candidate Gene ◽  
Ecological Model ◽  
Phosphoglucose Isomerase ◽  
Melitaea Cinxia ◽  
Model Species ◽  
Glanville Fritillary Butterfly

scholarly journals Improved chromosome-level genome assembly of the Glanville fritillary butterfly (Melitaea cinxia) integrating Pacific Biosciences long reads and a high-density linkage map

GigaScience ◽  
2022 ◽  
Vol 11 (1) ◽  
Author(s):  
Olli-Pekka Smolander ◽  
Daniel Blande ◽  
Virpi Ahola ◽  
Pasi Rastas ◽  
Jaakko Tanskanen ◽  
...  
Keyword(s):  
Linkage Map ◽  
Metapopulation Dynamics ◽  
Melitaea Cinxia ◽  
Pacific Biosciences ◽  
Final Assembly ◽  
Long Reads ◽  
Glanville Fritillary Butterfly ◽  
Gene Models ◽  
High Density Linkage Map ◽  
Chromosome Level

Abstract Background The Glanville fritillary (Melitaea cinxia) butterfly is a model system for metapopulation dynamics research in fragmented landscapes. Here, we provide a chromosome-level assembly of the butterfly's genome produced from Pacific Biosciences sequencing of a pool of males, combined with a linkage map from population crosses. Results The final assembly size of 484 Mb is an increase of 94 Mb on the previously published genome. Estimation of the completeness of the genome with BUSCO indicates that the genome contains 92–94% of the BUSCO genes in complete and single copies. We predicted 14,810 genes using the MAKER pipeline and manually curated 1,232 of these gene models. Conclusions The genome and its annotated gene models are a valuable resource for future comparative genomics, molecular biology, transcriptome, and genetics studies on this species.

scholarly journals Modelling single nucleotide effects in phosphoglucose isomerase on dispersal in the Glanville fritillary butterfly: coupling of ecological and evolutionary dynamics

2009 ◽  
Vol 364 (1523) ◽  
pp. 1519-1532 ◽  
Author(s):  
Chaozhi Zheng ◽  
Otso Ovaskainen ◽  
Ilkka Hanski
Keyword(s):  
Evolutionary Dynamics ◽  
Extinction Risk ◽  
Network Size ◽  
Phosphoglucose Isomerase ◽  
Landscape Matrix ◽  
Isolated Populations ◽  
Single Nucleotide ◽  
Spatially Correlated ◽  
Demographic Dynamics ◽  
Glanville Fritillary Butterfly

Dispersal comprises a complex life-history syndrome that influences the demographic dynamics of especially those species that live in fragmented landscapes, the structure of which may in turn be expected to impose selection on dispersal. We have constructed an individual-based evolutionary sexual model of dispersal for species occurring as metapopulations in habitat patch networks. The model assumes correlated random walk dispersal with edge-mediated behaviour (habitat selection) and spatially correlated stochastic local dynamics. The model is parametrized with extensive data for the Glanville fritillary butterfly. Based on empirical results for a single nucleotide polymorphism (SNP) in the phosphoglucose isomerase ( Pgi ) gene, we assume that dispersal rate in the landscape matrix, fecundity and survival are affected by a locus with two alleles, A and C, individuals with the C allele being more mobile. The model was successfully tested with two independent empirical datasets on spatial variation in Pgi allele frequency. First, at the level of local populations, the frequency of the C allele is the highest in newly established isolated populations and the lowest in old isolated populations. Second, at the level of sub-networks with dissimilar numbers and connectivities of patches, the frequency of C increases with decreasing network size and hence with decreasing average metapopulation size. The frequency of C is the highest in landscapes where local extinction risk is high and where there are abundant opportunities to establish new populations. Our results indicate that the strength of the coupling of the ecological and evolutionary dynamics depends on the spatial scale and is asymmetric, demographic dynamics having a greater immediate impact on genetic dynamics than vice versa.

scholarly journals Improved chromosome level genome assembly of the Glanville fritillary butterfly (Melitaea cinxia) based on SMRT Sequencing and linkage map

2020 ◽  
Author(s):  
Daniel Blande ◽  
Olli-Pekka Smolander ◽  
Virpi Ahola ◽  
Pasi Rastas ◽  
Jaakko Tanskanen ◽  
...  
Keyword(s):  
Linkage Map ◽  
Model System ◽  
Metapopulation Dynamics ◽  
Melitaea Cinxia ◽  
Smrt Sequencing ◽  
Final Assembly ◽  
Glanville Fritillary Butterfly ◽  
Gene Models ◽  
Chromosome Level

AbstractThe Glanville fritillary (Melitaea cinxia) butterfly is a long-term model system for metapopulation dynamics research in fragmented landscapes. Here, we provide a chromosome level assembly of the butterfly’s genome produced from Pacific Biosciences sequencing of a pool of males, combined with a linkage map from population crosses. The final assembly size of 484 Mb is an increase of 94 Mb on the previously published genome. Estimation of the completeness of the genome with BUSCO, indicates that the genome contains 93 - 95% of the BUSCO genes in complete and single copies. We predicted 14,830 gene models using the MAKER pipeline and manually curated 1,232 of these gene models. The genome and its annotated gene models are a valuable resource for future comparative genomics, molecular biology, transcriptome and genetics studies on this species.

scholarly journals Fitness differences associated with Pgi SNP genotypes in the Glanville fritillary butterfly (Melitaea cinxia)

2008 ◽  
Vol 22 (2) ◽  
pp. 367-375 ◽  
Author(s):  
L. ORSINI ◽  
C. W. WHEAT ◽  
C. R. HAAG ◽  
J. KVIST ◽  
M. J. FRILANDER ◽  
...  
Keyword(s):  
Melitaea Cinxia ◽  
Glanville Fritillary Butterfly

Acetylcholinesterase Genes in the Glanville Fritillary Butterfly (Melitaea cinxia, Lepidoptera: Nymphalidae)

2015 ◽  
Vol 88 (3) ◽  
pp. 340-353 ◽  
Author(s):  
Jialian Li ◽  
Shiqi Luo ◽  
Chang Shu ◽  
Chongren Xu ◽  
Rongjiang Wang
Keyword(s):  
Melitaea Cinxia ◽  
Glanville Fritillary Butterfly

An Experimental Study of Migration in the Glanville Fritillary Butterfly Melitaea cinxia

10.2307/5677 ◽  
1996 ◽  
Vol 65 (6) ◽  
pp. 791 ◽  
Author(s):  
Mikko Kuussaari ◽  
Marko Nieminen ◽  
Ilkka Hanski
Keyword(s):  
Experimental Study ◽  
Melitaea Cinxia ◽  
Glanville Fritillary Butterfly

scholarly journals Roundup causes embryonic development failure and alters metabolic pathways and gut microbiota functionality in non-target species

Microbiome ◽  
2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Antonio Suppa ◽  
Jouni Kvist ◽  
Xiaojing Li ◽  
Vignesh Dhandapani ◽  
Hanan Almulla ◽  
...  
Keyword(s):  
Drinking Water ◽  
Embryonic Development ◽  
Gut Microbiota ◽  
Chronic Exposure ◽  
Ecological Model ◽  
Toxicity Tests ◽  
Model Species ◽  
Genome Wide ◽  
Development Failure ◽  
The Impact

Abstract Background Research around the weedkiller Roundup is among the most contentious of the twenty-first century. Scientists have provided inconclusive evidence that the weedkiller causes cancer and other life-threatening diseases, while industry-paid research reports that the weedkiller has no adverse effect on humans or animals. Much of the controversial evidence on Roundup is rooted in the approach used to determine safe use of chemicals, defined by outdated toxicity tests. We apply a system biology approach to the biomedical and ecological model species Daphnia to quantify the impact of glyphosate and of its commercial formula, Roundup, on fitness, genome-wide transcription and gut microbiota, taking full advantage of clonal reproduction in Daphnia. We then apply machine learning-based statistical analysis to identify and prioritize correlations between genome-wide transcriptional and microbiota changes. Results We demonstrate that chronic exposure to ecologically relevant concentrations of glyphosate and Roundup at the approved regulatory threshold for drinking water in the US induce embryonic developmental failure, induce significant DNA damage (genotoxicity), and interfere with signaling. Furthermore, chronic exposure to the weedkiller alters the gut microbiota functionality and composition interfering with carbon and fat metabolism, as well as homeostasis. Using the “Reactome,” we identify conserved pathways across the Tree of Life, which are potential targets for Roundup in other species, including liver metabolism, inflammation pathways, and collagen degradation, responsible for the repair of wounds and tissue remodeling. Conclusions Our results show that chronic exposure to concentrations of Roundup and glyphosate at the approved regulatory threshold for drinking water causes embryonic development failure and alteration of key metabolic functions via direct effect on the host molecular processes and indirect effect on the gut microbiota. The ecological model species Daphnia occupies a central position in the food web of aquatic ecosystems, being the preferred food of small vertebrates and invertebrates as well as a grazer of algae and bacteria. The impact of the weedkiller on this keystone species has cascading effects on aquatic food webs, affecting their ability to deliver critical ecosystem services.

Sign in / Sign up

Export Citation Format

Share Document