scholarly journals Genetic Diversity of Chinese Indigenous Pig Breeds in Shandong Province Using Microsatellite Markers

2010 ◽  
Vol 24 (1) ◽  
pp. 28-36 ◽  
Author(s):  
J. Y. Wang ◽  
J. F. Guo ◽  
Q. Zhang ◽  
H. M. Hu ◽  
H. C. Lin ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Shandong Province ◽  
Pig Breeds

scholarly journals Genetic Diversity of 10 Indigenous Pig Breeds in China by Using Microsatellite Markers

2004 ◽  
Vol 17 (9) ◽  
pp. 1219-1222 ◽  
Author(s):  
X. Wang ◽  
H. H. Cao ◽  
S. M. Geng ◽  
H. B. Li
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Pig Breeds

scholarly journals Genetic diversity of Ukrainian local pig breeds based on microsatellite markers

2018 ◽  
Vol 9 (2) ◽  
pp. 177-182 ◽  
Author(s):  
S. S. Kramarenko ◽  
S. I. Lugovoy ◽  
V. R. Kharzinova ◽  
V. Y. Lykhach ◽  
A. S. Kramarenko ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Microsatellite Markers ◽  
Pig Breeds ◽  
Genetic Groups ◽  
The Mean ◽  
Living Material ◽  
The Individual ◽  
High Degree ◽  
Private Alleles

Preserving the current diversity of the living material on Earth is fundamental for the survival of future generations . A study was conducted to investigate the genetic diversity of Ukrainian local pig breeds. A total of 350 pigs representing five local pig breeds from Ukraine (Mirgorod – MIR, Poltava Meat – PM, Ukrainian Meat – UM, Ukrainian White Steppe – UWS and Ukrainian Spotted Steppe – USS) and one commercial breed (Duroc, DUR) were sampled. Twelve microsatellite loci (SW24, S0155, SW72, SW951, S0386, S0355, SW240, SW857, S0101, SW936, SW911 and S0228) were selected and belong to the list of microsatellite markers recommended by ISAG. The results indicate that there exists, in general, a high degree of genetic variability within the five Ukrainian local pig breeds. However, the genetic variability in the MIR and PM breeds was significantly lower (mean Na = 2.92–3.92; Ho = 0.382–0.411; FIS = 0.178–0.184) than in the other three Ukrainian local pig breeds – UM, UWS and USS (mean Na = 5.00–8.42; Ho = 0.549–0.668; FIS = 0.027–0.066). Thirty-four private alleles were identified among the six analyzed genetic groups which were distributed between 11 of the 12 loci. A high number of alleles typical for the breed (private alleles) was observed in Duroc pigs – 9 alleles did not occur in Ukrainian local pig breeds. The HWE test showed that all of the polymorphic loci deviated from HWE (P < 0.05) in at least one population. Loci S0355 (5), S0386 (4) and SW24 (4) presented a higher number of populations in imbalance. The mean FST showed that approximately 77.8% of the genetic variation was within-population and 12.2% was across the populations. The five Ukrainian local breeds were classified into two major groups, according to the phylogenetic tree, which was based on standard genetic distance. Overall, we found that 92.6% of the individual pigs were correctly assigned (324 out of 350) to the respective breed of origin, which is likely a consequence of the well-defined breed structure. Probabilities from the allocation test of individuals for the six pig genetic groups were estimated with Structure Harvester. In cluster 1 the highest grouping probabilities were found for the MIR (0.917) and PM (0.750) breeds. Local breeds UM (0.824) and USS (0.772) were grouped in cluster 2. Cluster 3 was related to the local pig breed USW (0.873). Cluster 4 presented high allocation probabilities for the commercial pig breed Duroc (0.924). The obtained results are important for the future conservation of Ukrainian local pig breeds.

Genetic diversity of autochthonous pig breeds analyzed by microsatellite markers and mitochondrial DNA D-loop sequence polymorphism

2018 ◽  
Vol 30 (3) ◽  
pp. 242-251 ◽  
Author(s):  
Kristina Gvozdanović ◽  
Vladimir Margeta ◽  
Polona Margeta ◽  
Ivona Djurkin Kušec ◽  
Dalida Galović ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Microsatellite Markers ◽  
Sequence Polymorphism ◽  
Pig Breeds ◽  
Loop Sequence ◽  
D Loop

Genetic diversity within and between European pig breeds using microsatellite markers

2006 ◽  
Vol 37 (3) ◽  
pp. 189-198 ◽  
Author(s):  
M. SanCristobal ◽  
C. Chevalet ◽  
C. S. Haley ◽  
R. Joosten ◽  
A. P. Rattink ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Pig Breeds

Genetic diversity of Brazilian pig breeds evidenced by microsatellite markers

2009 ◽  
Vol 123 (1) ◽  
pp. 8-15 ◽  
Author(s):  
B.P. Sollero ◽  
S.R. Paiva ◽  
D.A. Faria ◽  
S.E.F. Guimarães ◽  
S.T.R. Castro ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Pig Breeds

Genetic diversity based on microsatellite markers in five Nile tilapia strains

2009 ◽  
Vol 17 (1) ◽  
pp. 82 ◽  
Author(s):  
Liang Hongwei ◽  
Li Zhong ◽  
Luo Xiangzhong ◽  
Wang Changzhong ◽  
Hu Guangfu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Nile Tilapia

scholarly journals Genetic diversity within and among Pinus pinaster populations: comparison between AFLP and microsatellite markers

Heredity ◽  
2001 ◽  
Vol 86 (4) ◽  
pp. 469-479 ◽  
Author(s):  
Stéphanie Mariette ◽  
David Chagné ◽  
Céline Lézier ◽  
Patrick Pastuszka ◽  
Annie Raffin ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Pinus Pinaster

scholarly journals Genetic diversity and population structure of date palms (Phoenix dactylifera L.) in Ethiopia using microsatellite markers

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Workia Ahmed ◽  
Tileye Feyissa ◽  
Kassahun Tesfaye ◽  
Sumaira Farrakh
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Markers ◽  
Information Content ◽  
Date Palm ◽  
Polymorphic Information Content ◽  
Phoenix Dactylifera ◽  
Mean Value ◽  
Date Palms ◽  
Expected Heterozygosity

Abstract Background Date palm tree (Phoenix dactylifera L.) is a perennial monocotyledonous plant belonging to the Arecaceae family, a special plant with extraordinary nature that gives eminent contributions in agricultural sustainability and huge socio-economic value in many countries of the world including Ethiopia. Evaluation of genetic diversity across date palms at DNA level is very important for breeding and conservation. The result of this study could help to design for genetic improvement and develop germplasm introduction programmes of date palms mainly in Ethiopia. Results In this study, 124 date palm genotypes were collected, and 10 polymorphic microsatellite markers were used. Among 10 microsatellites, MPdCIR085 and MPdCIR093 loci showed the highest value of observed and expected heterozygosity, maximum number of alleles, and highest polymorphic information content values. A total of 112 number of alleles were found, and the mean number of major allele frequency was 0.26, with numbers ranging from 0.155 (MPdCIR085) to 0.374 (MPdCIR016); effective number of alleles with a mean value of 6.61, private alleles ranged from 0.0 to 0.65; observed heterozygosity ranged from 0.355 to 0.726; expected heterozygosity varied from 0.669 to 0.906, polymorphic information content with a mean value of 0.809; fixation index individuals relative to subpopulations ranged from 0.028 for locus MPdCIR032 to 0.548 for locus MPdCIR025, while subpopulations relative to total population value ranged from − 0.007 (MPdCIR070) to 0.891 (MPdCIR015). All nine accesstions, neighbour-joining clustering analysis, based on dissimilarity coefficient values were grouped into five major categories; in population STRUCTURE analysis at highest K value, three groups were formed, whereas DAPC separated date palm genotypes into eight clusters using the first two linear discriminants. Principal coordinate analysis was explained, with a 17.33% total of variation in all populations. Generally, the result of this study revealed the presence of allele variations and high heterozygosity (> 0.7) in date palm genotypes. Conclusions Microsatellites (SSR) are one of the most preferable molecular markers for the study of genetic diversity and population structure of plants. In this study, we found the presence of genetic variations of date palm genotypes in Ethiopia; therefore, these genetic variations of date palms is important for crop improvement and conservation programmes; also, it will be used as sources of information to national and international genbanks.

scholarly journals Microsatellite markers-based characterisation of elephant grass (Pennisetum purpureum) harvested from selected locations in South-West Nigeria

2020 ◽  
Vol 37 (1) ◽  
pp. 38-45
Author(s):  
O.A. Okukenu ◽  
A.A. Olajide ◽  
P.A. Dele ◽  
M. Wheto ◽  
B.T. Akinyemi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Dna Extraction ◽  
Morphological Characterization ◽  
Ethanol Solution ◽  
Pennisetum Purpureum ◽  
Elephant Grass ◽  
South West ◽  
Ssr Primers ◽  
Genetic Index

This study was carried out to characterise Pennisetum purpureum harvested from some selected locations in S outh-W estern Nigeria using microsatellite markers. Leaf parts of growing young elephant grass (Pennisetum purpureum) were harvested and immediately preserved in ethanol solution before DNA extraction. Two (2) SSR primers (CTM59 and Xtxp278) were used to assess genetic diversity in Pennisetum purpureum. The result shows that 72% of the molecular variations in the elephant grass exists within the population with 28% among the population; there were no unique characteristics among the Nine (9) populations. Nei genetic index ranged from 0.067 (lowest) observed between Isokan and Odeda populations to 0.158 (highest), between Ifedore and Ikoyi Populations. Morphological characterization showed moderate diversity with two major clusters and one minor cluster. Keyword: Elephant grass; cultivars; locations; markers

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