scholarly journals Effects of Heart Fatty Acid-binding Protein Genotype on Intramuscular Fat Content in Duroc Pigs Selected for Meat Production and Meat Quality Traits

2007 ◽  
Vol 20 (5) ◽  
pp. 622-626 ◽  
Author(s):  
Yoshinobu Uemoto ◽  
Keiichi Suzuki ◽  
Eiji Kobayashi ◽  
Syushi Sato ◽  
Tomoya Shibata ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Meat Quality ◽  
Fatty Acid Binding Protein ◽  
Fat Content ◽  
Meat Production ◽  
Quality Traits ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Intramuscular Fat Content ◽  
Protein Genotype

scholarly journals Variability in the leptin, leptin receptor and heart fatty acid binding protein genes in relationship with meat quality traits in pigs

2005 ◽  
Vol 53 (4) ◽  
pp. 87-94
Author(s):  
Renata Mikolášová ◽  
Tomáš Urban
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Leptin Receptor ◽  
Binding Protein ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Large White ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Intramuscular Fat Content

The leptin (LEP-HinfI), leptin receptor (LEPR-HpaII) and heart fatty acid binding protein (H-FABP-HinfI) genes and their genotypes combination (LEP-HinfI *LEPR-HpaII) were tested for associations with the pH1, pH24, myoglobin content (mg/100 g), intramuscular fat content (%) and remission (%). The genotypes were determined in Large White, Landrace and Duroc breeds (n = 106, 56 and 4, respectively). The allele frequencies were: LEP-HinfI: C = 0.133 T = 0.867; LEPR-HpaII: A = 0.331 B = 0.669; H-FABP-HinfI: H = 0.745 h = 0.255. The populations of breeds were in the genetic equilibrium according to the χ2 test in the tested loci. The combinations of LEP-HinfI and LEPR-HpaII were significantly associated with the pH24 and remission. The H-FABP-HinfI locus was significantly associated with intramuscular fat content.

Sequence characterization, tissue-specific expression and polymorphism of the porcine intestinal-type fatty acid binding protein gene

2006 ◽  
Vol 86 (4) ◽  
pp. 561-567
Author(s):  
Y. Z. Jiang ◽  
X. W. Li
Keyword(s):  
Fatty Acids ◽  
Small Intestine ◽  
Fatty Acid ◽  
Association Analysis ◽  
Fatty Acid Binding Protein ◽  
Fat Content ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Intramuscular Fat Content ◽  
Fabp Gene

The intestinal fatty-acid-binding protein (IFABP) shows binding specificity for long-chain fatty acids and is proposed to be involved in the uptake of dietary fatty acids and their intracellular transport. In this study, the full-length cDNA of porcine I-FABP gene was obtained by the rapid amplification of cDNA ends (RACE). The nucleotide sequence and the predicted protein sequence share high sequence identity with its mammalian counterparts. Northern hybridization and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) revealed that porcine I-FABP is expressed in all 12 tissues studied (heart, brain, kidney, skeletal muscle, testis, liver, skin, small intestine, fat, stomach, lymph and pituitary), but a transcript of approximate 620 bp is more abundant in small intestine than in other tissues. The full-length genomic DNA of the porcine I-FABP gene was amplified by PCR. The coding region of the pig IFABP gene is organized in four exons and spans an approximate 3.5-kb genomic region. Comparative sequencing of four pig breeds revealed a single nucleotide polymorphism (SNP) within exon 1 of which an A→G substitution at codon 21 changes a codon for lysine into a codon for arginine. The distribution of allele and genotype frequencies differed significantly between indigenous Chinese Zang, Dahe and Yanan breeds (higher frequencies of A and AA) and Western Large White breed (higher frequencies of G and GG, P < 0.01). The association analysis using five pig populations suggested that A21G polymorphism was associated with intramuscular fat content, indicating that the I-FABP gene A21G SNP can be a potential molecular marker for intramuscular fat content. Key words: Association analysis, cloning, gene expression, I-FABP gene, polymorphism, porcine

The adipocyte fatty acid-binding protein locus: characterization and association with intramuscular fat content in pigs

1998 ◽  
Vol 9 (12) ◽  
pp. 1022-1026 ◽  
Author(s):  
Frans Gerbens ◽  
Aswin Jansen ◽  
Anton J.M. van Erp ◽  
Frank Harders ◽  
Theo H.E. Meuwissen ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Protein Locus ◽  
Intramuscular Fat Content

An assessment of sire-breed effects on carcass and meat quality traits of lambs at the ages of 40 and 100 days from Comisana ewes crossed with Suffolk or Bergamasca rams

2018 ◽  
Vol 58 (10) ◽  
pp. 1794 ◽  
Author(s):  
Pasquale De Palo ◽  
Aristide Maggiolino ◽  
Pasquale Centoducati ◽  
Giovanna Calzaretti ◽  
Edmondo Ceci ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Meat Quality ◽  
Saturated Fatty Acid ◽  
Carcass Weight ◽  
Meat Production ◽  
Quality Traits ◽  
Breed Group ◽  
Sire Breed ◽  
Dairy Breed ◽  
Intramuscular Fat Content

The aim was to compare lambs at two different ages at slaughter (40 vs 100 days) and three breeds or crossbreeds including an Italian dairy breed (Comisana (Com)) and its crosses with the two meat breeds of Bergamasca (Ber × Com) and Suffolk (Suf × Com) with 20 male lambs per age per breed group. At both slaughter ages, crossbreeds were more productive, with higher liveweight (P = 0.02), carcass weight (P = 0.03) and dressing percentage (P = 0.04) than the Com group. Meat from the younger lambs showed lower C10:0, C14:0 and C22:0 (P < 0.05) and higher C18:1 (P < 0.01) concentrations, resulting in higher monounsaturated fatty acid (P < 0.01) and lower saturated fatty acid (P < 0.01) content. Moreover, their meat has lower redness (a*), yellowness (b*) and chroma (C*) values (P < 0.01). Crossbreeding dairy breed ewes with sires of high meat production breeds improves liveweight and carcass weight. Meat from younger lambs had lower intramuscular fat content and higher concentrations of polyunsaturated fatty acid.

scholarly journals Effects of polymorphism of myostatin and fatty acid-binding protein 4 genes on the chemical composition of meat in cull female Aceh cattle

2020 ◽  
Vol 13 (7) ◽  
pp. 1334-1343
Author(s):  
Al Azhar ◽  
Muslim Akmal ◽  
Muhammad Hambal ◽  
Mustafa Sabri ◽  
Teuku Shaddiq Rosa
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
National Standard ◽  
Meat Production ◽  
Chemical Parameters ◽  
Fatty Acid Binding ◽  
Biceps Femoris Muscle ◽  
Acid Binding Protein ◽  
Spin Column

Aim: This study aimed to investigate the association of single nucleotide polymorphism of the myostatin (MSTN) and fatty acid-binding protein 4 (FABP4) genes on the total water, ash, fat, protein, and cholesterol contents of sirloin (gluteus medius muscle) and silverside (biceps femoris muscle) meats of cull female Aceh cattle. Materials and Methods: This analysis covered a total of 27 cull female Aceh cattle slaughtered at the Animal Slaughterhouse of Banda Aceh that was purposively selected based on hair color referred to the criteria described in the Decree of Ministry of Agriculture of the Republic of Indonesia. Genomic DNA was extracted from 25 mg of fresh meat using the spin column method before subjected to a polymerase chain reaction amplification using primer sets specific for 1346-bp and 275- bp fragments of MSTN and FABP4, respectively. A 4-h digestion reaction was done separately for the MSTN/HaeIII and FABP4/NlaIII loci genotyping. The total protein, ash, and fat of the meat were measured using the Indonesian National Standard (SNI) methods whereas its cholesterol content was determined using the AOAC method. The association between each polymorphism and the variation in meat chemical parameters was analyzed using the Pearson correlation test. Results: The results showed that the MSTN/HaeIII locus was polymorphic in Aceh cattle, but the FABP4/NlaIII locus was monomorphic. Meat chemical parameters were not influenced by different commercial cuts and MSTN genotypes, showing that there was no association between different commercial cuts, cattle hair colors, and MSTN/HaeIII and FABP4/NlaIII markers with the meat chemical parameters in Aceh cattle. Conclusion: These results suggest that focusing on the novel effects of MSTN and FABP4 gene polymorphisms on meat production traits might not be useful for marker-assisted selection in Aceh cattle.

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