scholarly journals Effects of Dietary Hizikia fusiformis on Growth and Immune Responses in Juvenile Olive Flounder (Paralichthys olivaceus)

2006 ◽  
Vol 19 (12) ◽  
pp. 1769-1775 ◽  
Author(s):  
Minh Anh Pham ◽  
Kyeong-Jun Lee ◽  
Bong-Joo Lee ◽  
Se-Jin Lim ◽  
Sung-Sam Kim ◽  
...  
Keyword(s):  
Immune Responses ◽  
Paralichthys Olivaceus ◽  
Olive Flounder ◽  
Hizikia Fusiformis ◽  
Olive Flounder Paralichthys Olivaceus

scholarly journals Elucidating the Functional Roles of Helper and Cytotoxic T Cells in the Cell-Mediated Immune Responses of Olive Flounder (Paralichthys olivaceus)

2021 ◽  
Vol 22 (2) ◽  
pp. 847
Author(s):  
Jae Wook Jung ◽  
Ae Rin Lee ◽  
Jaesung Kim ◽  
Young Rim Kim ◽  
Jassy Mary S. Lazarte ◽  
...  
Keyword(s):  
T Cells ◽  
T Lymphocytes ◽  
Immune Responses ◽  
Cytotoxic T Cells ◽  
Paralichthys Olivaceus ◽  
Olive Flounder ◽  
Control Group ◽  
Adaptive Immune ◽  
Cd8 T Lymphocytes ◽  
Olive Flounder Paralichthys Olivaceus

In higher vertebrates, helper and cytotoxic T cells, referred to as CD4 and CD8 T lymphocytes, respectively, are mainly associated with adaptive immunity. The adaptive immune system in teleosts involves T cells equivalent to those found in mammals. We previously generated monoclonal antibodies (mAbs) against olive flounder (Paralichthys olivaceus) CD4 T cells, CD4-1 and CD4-2, and used these to describe the olive flounder’s CD4 Tcell response during a viral infection. In the present study, we successfully produced mAbs against CD8 T lymphocytes and their specificities were confirmed using immuno-blotting, immunofluorescence staining, flow cytometry analysis andreverse transcription polymerase chain reaction (RT-PCR). The results showed that these mAbs are specific for CD8 T lymphocytes. We also investigated variations in CD4 and CD8 T cells populations, and analyzed the expression of immune-related genes expressed by these cells in fish infected with nervous necrosis virus or immunized with thymus dependent and independent antigens. We found that both CD4 and CD8 T lymphocyte populations significantly increased in these fish and Th1-related genes were up-regulated compared to the control group. Collectively, these findings suggest that the CD4 and CD8 T lymphocytes in olive flounder are similar to the helper and cytotoxic T cells found in mammals, and Th1 and cytotoxic immune responses are primarily involved in the early adaptive immune response against extracellular antigens.

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