scholarly journals Microsatellite DNA Typing Using 16 Markers for Parentage Verification of the Korean Native Horse

2004 ◽  
Vol 17 (6) ◽  
pp. 750-754 ◽  
Author(s):  
G. J. Cho ◽  
B. W. Cho
2015 ◽  
Vol 8 (7) ◽  
pp. 848-854 ◽  
Author(s):  
Anoop Singh Yadav ◽  
Kritika Gahlot ◽  
Gyan Chand Gahlot ◽  
Mohd Asraf ◽  
Mohan Lal Yadav

2020 ◽  
Vol 42 (2) ◽  
pp. 240
Author(s):  
Peter B. S. Spencer ◽  
Serina McConnell ◽  
Diana Prada ◽  
J. A. (Tony) Friend

The numbat (Myrmecobius fasciatus) is an endangered and peculiar marsupial with a diet that consists almost exclusively of termites. This study developed a parentage-testing system for numbats using microsatellite markers. Nineteen loci detected 143 alleles, with 4–13 alleles/locus and average expected heterozygosity of 77% (range 0.665–0.855). The total parentage exclusion probability was >0.9999 (given only the genotype of the offspring), >0.9999 for excluding a candidate parent from the parentage of an arbitrary offspring (given the genotype of the offspring and parent) and the probability of identity for full-sibs was 4.6×10–9. Overall, these microsatellites offer a simple and highly informative marker-set for parentage identification in numbats.


2010 ◽  
Vol 41 (6) ◽  
pp. 662-665 ◽  
Author(s):  
P. B. S. Spencer ◽  
K. J. Wilson ◽  
A. Tinson

2010 ◽  
Vol 9 (11) ◽  
pp. 1565-1569 ◽  
Author(s):  
Zou Jian-Min ◽  
Wei Feng-Ying ◽  
Shu Jing-Ting ◽  
Song Wei-Tao ◽  
Han Wei ◽  
...  

2006 ◽  
Vol 85 (3) ◽  
pp. 165-170 ◽  
Author(s):  
M. Sodhi ◽  
M. Mukesh ◽  
B. Prakash ◽  
S. P. S. Ahlawat ◽  
R. C. Sobti

1994 ◽  
Vol 71 (05) ◽  
pp. 651-654 ◽  
Author(s):  
Rainer Kalb ◽  
Sentot Santoso ◽  
Katja Unkelbach ◽  
Volker Kiefel ◽  
Christian Mueller-Eckhardt

SummaryAlloimmunization against the human platelet alloantigen system Br (HPA-5) is the second most common cause of neonatal alloimmune thrombocytopenia (NAIT) in Caucasian populations. We have recently shown that a single base polymorphism at position 1648 on platelet mRNA coding for GPIa results in an aminoacid substitution at position 505 on the mature GPIa which is associated with the two serological defined Br phenotypes.Since DNA-typing of platelet alloantigens offers possibilities for useful clinical applications, we designed genomic DNA-based restriction fragment length polymorphism (RFLP) typing for Br alloantigens. To establish this technique we analyzed the genomic organization of GPIa adjacent to the polymorphic base. Using the polymerase chain reaction (PCR) of blood cell DNA we have identified two introns (approximately 1.7 and 1.9 kb) flanking a 144 bp coding sequence of the GPIa gene encompassing the polymorphic base 1648. Based on the in- tron sequence, a PCR primer was constructed to amplify a 274 bp fragment which was used for allele-specific RFLP to determine the Br genotypes. The results of RFLP analysis using Mnll endonuclease obtained from 15 donors (2 Br37*, 2 Br^ and 11 Brb/b) correlate perfectly with serological typing by monoclonal antibody-specific immobilization of platelet antigens (MAIPA) assay.


2016 ◽  
Vol 18 (1) ◽  
pp. 27-32 ◽  
Author(s):  
V. Dzitsiuk ◽  
◽  
S. Kruhlyk ◽  
V. Spyrydonov ◽  
◽  
...  

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