scholarly journals Effect of Extenders and Temperatures on Sperm Viability and Fertilizing Capacity of Harbin White Boar Semen during Long-term Liquid Storage

2004 ◽  
Vol 17 (11) ◽  
pp. 1501-1508 ◽  
Author(s):  
J. B. Zhou ◽  
K. Z. Yue ◽  
M. J. Luo ◽  
Z. L. Chang ◽  
H. Liang ◽  
...  
Keyword(s):  
Sperm Viability ◽  
Liquid Storage ◽  
Boar Semen

scholarly journals The effect of royal jelly on boar sperm viability and motility during liquid storage for 96 hours

2020 ◽  
Vol 89 (1) ◽  
pp. 47-53
Author(s):  
Aiste Iljenkaite ◽  
Sigita Kerziene ◽  
Agila Dauksiene ◽  
Zoja Mikniene ◽  
Henrikas Žilinskas ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Storage Time ◽  
Royal Jelly ◽  
Incubation Temperature ◽  
Ph Value ◽  
Protective Effects ◽  
Sperm Viability ◽  
Liquid Storage ◽  
Sperm Plasma Membrane ◽  
Boar Semen

The current study was carried out to investigate the protective effects of royal jelly supplementation on sperm motility, viability and pH value during the liquid storage of boar semen at 16 °C and 4 °C, at various periods of time (0, 24, 48, 72 and 96 h). Semen samples were collected from 11 boars, diluted with a long-term extender and supplemented with different concentration of royal jelly (0%, 0.5%, 1% and 2%) at a final concentration of 50 × 106 sperm/ml. In the laboratory, the semen was assessed for sperm morphology, viability (eosin-nigrosin staining), subjective motility and objective sperm motility by sperm class analyzer. In total, 396 tests for sperm viability and motility were performed. The longer storage time and the lower incubation temperature showed lower sperm motility and viability results. The results showed that royal jelly supplementation at 1% concentrations protected the functionality of the sperm plasma membrane during the liquid storage time of 96 h at 16 °C. Sperm subjective and objective motility results in samples stored at 4 °C decreased with higher royal jelly concentrations and longer storage time, and differ significantly from the results in samples stored at 16 °C (P < 0.05). Our data showed that royal jelly supplementation at lower concentrations can improve boar semen motility and viability parameters during liquid storage at 16 °C for 96 h.

Effect of commercial long-term extenders on metabolic activity and membrane integrity of boar spermatozoa stored at 17°C

2013 ◽  
Vol 16 (3) ◽  
pp. 517-525 ◽  
Author(s):  
A. Dziekońska ◽  
L. Fraser ◽  
A. Majewska ◽  
M. Lecewicz ◽  
Ł. Zasiadczyk ◽  
...  
Keyword(s):  
Metabolic Activity ◽  
Storage Time ◽  
Membrane Integrity ◽  
Prolonged Storage ◽  
Atp Content ◽  
Liquid Storage ◽  
Semen Storage ◽  
Boar Semen ◽  
Boar Spermatozoa

Abstract This study was aimed to analyze the metabolic activity and membrane integrity of boar spermatozoa following storage in long-term semen extenders. Boar semen was diluted with AndrohepR EnduraGuardTM (AeG), DILU-Cell (DC), SafeCell PlusTM (SCP) and Vitasem LD (VLD) extenders and stored for 10 days at 17oC. Parameters of the analyzed sperm metabolic activity included total motility (TMOT), progressive motility (PMOT), high mitochondrial membrane potential (MMP) and ATP content, whereas those of the membrane integrity included plasma membrane integrity (PMI) and normal apical ridge (NAR) acrosome. Extender type was a significant (P < 0.05) source of variation in all the analyzed sperm parameters, except for ATP content. Furthermore, the storage time had a significant effect (P < 0.05) on the sperm metabolic activity and membrane integrity during semen storage. In all extenders the metabolic activity and membrane integrity of the stored spermatozoa decreased continuously over time. Among the four analyzed extenders, AeG and SCP showed the best performance in terms of TMOT and PMI on Days 5, 7 and 10 of storage. Marked differences in the proportions of spermatozoa with high MMP were observed between the extenders, particularly on Day 10 of storage. There were not any marked differences in sperm ATP content between the extenders, regardless of the storage time. Furthermore, the percentage of spermatozoa with NAR acrosomes decreased during prolonged storage, being markedly lower in DC-diluted semen compared with semen diluted with either AeG or SCP extender. The results of this study indicated that components of the long-term extenders have different effects on the sperm functionality and prolonged semen longevity by delaying the processes associated with sperm ageing during liquid storage.

Seminal plasma affects sperm sex sorting in boars

2016 ◽  
Vol 28 (5) ◽  
pp. 556 ◽  
Author(s):  
Diego V. Alkmin ◽  
Inmaculada Parrilla ◽  
Tatiana Tarantini ◽  
David del Olmo ◽  
Juan M. Vazquez ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Holding Time ◽  
Oxygen Species ◽  
Plasma Membrane Fluidity ◽  
Progressive Motility ◽  
Liquid Storage ◽  
Boar Semen ◽  
Sorting Efficiency ◽  
Boar Spermatozoa

Two experiments were conducted in boar semen samples to evaluate how both holding time (24 h) and the presence of seminal plasma (SP) before sorting affect sperm sortability and the ability of sex-sorted spermatozoa to tolerate liquid storage. Whole ejaculate samples were divided into three aliquots immediately after collection: one was diluted (1 : 1, v/v) in Beltsville thawing solution (BTS; 50% SP); the SP of the other two aliquots was removed and the sperm pellets were diluted with BTS + 10% of their own SP (10% SP) or BTS alone (0% SP). The three aliquots of each ejaculate were divided into two portions, one that was processed immediately for sorting and a second that was sorted after 24 h storage at 15–17°C. In the first experiment, the ability to exhibit well-defined X- and Y-chromosome-bearing sperm peaks (split) in the cytometry histogram and the subsequent sorting efficiency were assessed (20 ejaculates). In contrast with holding time, the SP proportion influenced the parameters examined, as evidenced by the higher number of ejaculates exhibiting split and better sorting efficiency (P < 0.05) in semen samples with 0–10% SP compared with those with 50% SP. In a second experiment, the quality (viability, total and progressive motility) and functionality (plasma membrane fluidity and intracellular generation of reactive oxygen species) of sex-sorted spermatozoa were evaluated after 0, 72 and 120 h storage at 15–17°C (10 ejaculates). Holding time and SP proportion did not influence the quality or functionality of stored sex-sorted spermatozoa. In conclusion, a holding time as long as 24 h before sorting did not negatively affect sex sorting efficiency or the ability of sorted boar spermatozoa to tolerate long-term liquid storage. A high proportion of SP (50%) in the semen samples before sorting reduced the number of ejaculates to be sorted and negatively influenced the sorting efficiency, but did not affect the ability of sex-sorted spermatozoa to tolerate liquid storage.

Long-term liquid storage and reproductive evaluation of an innovative boar semen extender (Formula12 ® ) containing a non-reducing disaccharide and an enzymatic agent

2017 ◽  
Vol 180 ◽  
pp. 10-16 ◽  
Author(s):  
Carla Bresciani ◽  
Annalisa Bianchera ◽  
Ruggero Bettini ◽  
Annamaria Buschini ◽  
Laura Marchi ◽  
...  
Keyword(s):  
Liquid Storage ◽  
Boar Semen ◽  
Semen Extender

Melatonin and canthaxanthin enhances sperm viability and protect ram spermatozoa from oxidative stress during liquid storage at 4°C

Andrologia ◽  
2021 ◽  
Author(s):  
Shivendra Kumar Bhalothia ◽  
Jitendra Singh Mehta ◽  
Tapendra Kumar ◽  
Chandan Prakash ◽  
Thirumala Rao Talluri ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sperm Viability ◽  
Liquid Storage

scholarly journals Effect of fruit-juice on spermatozoa viability and lipid peroxidation of Red Sokoto bucks during liquid storage

2021 ◽  
Vol 41 (1) ◽  
pp. 16-27
Author(s):  
J. O. Daramola ◽  
T. A. Sorongbe ◽  
O. M. Onagbesan ◽  
A. V. Jegede ◽  
A. O. Ladokun ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Cell Damage ◽  
Egg Yolk ◽  
Protective Effects ◽  
Sperm Viability ◽  
Progressive Motility ◽  
Liquid Storage ◽  
Watermelon Juice ◽  
And Control

Antioxidants are linked with sperm viability because of their protective effects against cell damage during preservation. In order to enhance the life span of refrigerated buck semen, this study was carried out to determine the effect of fruit-rich antioxidants on spermatozoa viability and lipid peroxidation (LPO) of buck semen during liquid storage. Pooled semen from five Red Sokoto bucks was diluted with Tris-egg yolk based extender and supplemented each with juices from pawpaw tomato and watermelon at 0, 2.5, 5, 7.5 and 10/ 100 ml respectively. Following dilution, the semen samples were assessed subjectively after in vitro storage at 5°C for 24, 48, 72 and 96 hours as regards sperm motility, abnormalities, and acrosome status using a phase-contrast microscope. The concentration of malondialdehyde (MDA) as indices of lipid peroxidation (LPO) in the stored semen was measured in thiobarbituric acid reactive substances (TBARS) at 24, 48, 72 and 96 hours. The results showed highest progressive motility in watermelon juice at 2.5% (P<0.05) during the first 24 hours of storage while the lowest progressive motility was recorded at various levels of pawpaw juice (P<0.05). After 48 hours of storage, extender supplemented with watermelon and tomato juices had better progressive motility compared to control except 7.5% and 10%% of tomato juice (P<0.05). Irrespective of level of juice in the extender, the percentage of intact acrosome was similar among the various juices and control. The results showed that spermatozoa extended with watermelon juice had the lowest (P<0.05) percentage abnormality compared to other extenders at 24, 48, 72 and 96 hours of storage. Higher (P<0.05) percent spermatozoa abnormality compared to other fruit juices and control was observed at 72 and 96 hours of storage in spermatozoa extended with pawpaw juice. Significant reductions of MDA concentrations were achieved by addition of fruit-rich antioxidants to Tris-egg yolk based extender during the first 72 hours and the reduction was much pronounced in extender supplemented with pawpaw juice compared to control (P<0.05). The findings reveal that fruit-rich antioxidants from watermelon and tomato have protective ability to maintain sperm viability and to reduce concentration MDA of buck semen during liquid storage.

scholarly journals Implication of Polyhistidine, a Novel Apoptosis Inhibitor, in Inhibiting Lipopolysaccharide-Induced Apoptosis in Boar Sperm

Animals ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 719 ◽  
Author(s):  
Tianzeng Song ◽  
Yi Shi ◽  
Yangang Wang ◽  
Izhar Hyder Qazi ◽  
Christiana Angel ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Storage Conditions ◽  
Potential Implication ◽  
Liquid Storage ◽  
Boar Sperm ◽  
Tlr4 Agonist ◽  
Reasonable Evidence ◽  
Boar Semen ◽  
Induced Apoptosis ◽  
Apoptosis Inhibitor

Lipopolysaccharide (LPS) released from Gram-negative bacteria binds to toll-like receptor 4 (TLR4) and induces boar sperm apoptosis. Similarly, polyhistidine (pHis), a TLR4 agonist, can also bind to TLR4. We hypothesized that pHis could inhibit LPS-induced sperm apoptosis by competitively binding to TLR4 to then improve sperm quality. Therefore, the objective of this study was to examine whether pHis can inhibit LPS-induced sperm apoptosis and affect sperm quality. The results showed that the concentrations of bacterial colonies were significantly increased from 36 to 120 h under liquid storage conditions (p < 0.05); however, concentrations of LPS in boar semen showed a relatively constant trend (4.98 ± 1.55 EU/mL) following 120 h storage. The addition of 100 μg/mL pHis in the BTS extender significantly improved boar sperm motility and viability at 37 °C, and it significantly (p < 0.05) inhibited boar sperm apoptosis under liquid storage (17 °C) and at 37 °C incubation conditions. The co-treatment of LPS and pHis further confirmed that pHis played its role in inhibiting LPS-induced sperm apoptosis. In conclusion, our preliminary findings provide reasonable evidence that pHis could act as an inhibitor of LPS-induced apoptosis in boar sperm stored for longer periods of time. pHis might inhibit LPS-induced sperm apoptosis by competitively binding to TLR4. Nevertheless, further mechanistic studies are awaited to fully elucidate its potential implication in inhibiting LSP-induced apoptosis.

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