scholarly journals Transcriptomic analysis of the liver in aged laying hens with different intensity of brown eggshell color

2020 ◽  
Author(s):  
Gi ppeum Han ◽  
Jun-Mo Kim ◽  
Hwan Ku Kang ◽  
Dong Yong Kil
Keyword(s):  
Laying Hens ◽  
Transcriptomic Analysis ◽  
Eggshell Color

scholarly journals PSIV-3 Late-Breaking: Transcriptomic analysis in the liver of aged brown-egg laying hens with different intensity of eggshell color.

2018 ◽  
Vol 96 (suppl_3) ◽  
pp. 286-286
Author(s):  
G Han ◽  
D Kil ◽  
D Goo ◽  
G Park ◽  
J Kim ◽  
...  
Keyword(s):  
Laying Hens ◽  
Egg Laying ◽  
Transcriptomic Analysis ◽  
Eggshell Color

scholarly journals The Effect of the Type of Alternative Housing System, Genotype and Age of Laying Hens on Egg Quality

2018 ◽  
Vol 18 (2) ◽  
pp. 541-556 ◽  
Author(s):  
Zofia Sokołowicz ◽  
Józefa Krawczyk ◽  
Magdalena Dykiel
Keyword(s):  
Laying Hens ◽  
Egg Quality ◽  
Housing System ◽  
Color Intensity ◽  
Egg Weight ◽  
Blood Spots ◽  
Alternative Housing ◽  
Housing Systems ◽  
System Type ◽  
Eggshell Color

Abstract The present study investigated the effect of the type of alternative housing system, and genotype and age of laying hens on physical traits of egg shell and contents. It was demonstrated that alternative housing system type influenced egg weight and shape, and eggshell color and yolk color intensity. Eggs from free-range system were heavier and were characterized by more intense yolk color. No effect of alternative housing system type on albumen height, value of Haugh units (HU value) and presence of meat and blood spots was noted. Hen genotype had a significant effect on egg weight and eggshell color intensity in each of the alternative housing systems tested in this study. Hy-line Brown hens laid heavier eggs than hens of native breeds. Genotype was also observed to affect egg content traits (albumen height, HU values and presence of meat and blood spots). Independently of the type of alternative housing system, most blood and meat spots were noted in eggs of hens laying brown-shelled eggs, i.e. R-11 and Hy-line Brown layers. Laying hen age significantly impacted on egg weight, yolk percentage, eggshell traits (color intensity, weight, thickness and strength) and egg content traits (HU value, yolk weight and color intensity, presence of meat and blood spots). Older hens laid heavier eggs with a greater yolk percentage but with thinner eggshell.

scholarly journals PSVII-19 Transcriptomic analysis of the liver of laying hens fed diets containing supplemental fat at early laying stages

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 353-353
Author(s):  
Gi Ppeum Han ◽  
Geun Hyeon Park ◽  
Jong Hyuk Kim ◽  
Hyeon Seok Choi ◽  
Hwan Ku Kang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Molecular Mechanisms ◽  
Acid Metabolism ◽  
Laying Hens ◽  
Acid Synthesis ◽  
Transcriptomic Analysis ◽  
Cdna Libraries ◽  
Feeding Diets ◽  
Supplemental Fat ◽  
Liver Tissues

Abstract Feeding diets containing supplemental fat to laying hens is reported to ameliorate pathogenesis of fatty liver hemorrhagic syndrome (FLHS). However, molecular mechanisms for this positive effect have not been investigated. Thus, we conducted a transcriptomic analysis of the liver of laying hens fed diets containing supplemental fat at early laying stages. Two dietary treatments included basal diets with no supplemental fat and basal diets supplemented with 3.0% tallow. A total of 256 18-week-old Hy-line Brown laying hens were allotted to 1 of 2 treatments. Diets were fed to hens for 12 weeks. At the end of the experiment (30 weeks of age), 5 hens with similar BW per treatment were euthanized to collect liver tissues. The cDNA libraries were constructed with extracted RNA from the liver tissues, and sequenced using the Illumina Nextseq 500 sequencer. Genes with False Discovery Rate (FDR) < 0.05 were defined as differentially expressed genes (DEGs). Results indicated that a total of 951 DEGs were identified, with 483 being up-regulated and 468 being down-regulated in the liver of hens fed diets containing 3.0% tallow. The KEGG analysis revealed that the DEGs belong to several biological pathways such as cellular signaling pathways, carbon metabolism, glycolysis, gluconeogenesis, TCA cycle, amino acid metabolism, drug metabolism, and glycerophospholipid metabolism. Especially for fatty acid metabolism, the DEGs associated with fatty acid degradation (ECI2, ACSL1, HADHA, EHHADH, ACOX1, CPT1) were up-regulated, whereas those related to fatty acid synthesis (ACACA, ACSL5, FASN) were down-regulated in the liver of hens fed diets containing 3.0% tallow, which indicates that supplemental fat in diets may increase fat oxidation but decrease fat synthesis in the liver. These results provide the molecular insights for hepatic lipid metabolisms by feeding diets containing supplemental fat to laying hens at early laying stages.

scholarly journals Eggshell color in brown-egg laying hens — a review

2015 ◽  
Vol 94 (10) ◽  
pp. 2566-2575 ◽  
Author(s):  
S. Samiullah ◽  
J.R. Roberts ◽  
K. Chousalkar
Keyword(s):  
Laying Hens ◽  
Egg Laying ◽  
Eggshell Color

scholarly journals Transcriptomic and proteomic analyses of ovarian follicles reveal the role of VLDLR in chicken follicle selection

2020 ◽  
Author(s):  
Qiuyue Chen ◽  
Yiya Wang ◽  
Zemin Liu ◽  
Xiaoli Guo ◽  
Yi Sun ◽  
...  
Keyword(s):  
Laying Hens ◽  
Mrna Level ◽  
Ovarian Follicles ◽  
Transcriptomic Analysis ◽  
Differentially Expressed ◽  
Integrated Analysis ◽  
Differentially Expressed Proteins ◽  
Thecal Cells ◽  
Laying Performance ◽  
Follicle Selection

Abstract Background: Follicle selection in chickens refers to the process of selecting one follicle from a group of small yellow follicles (SY, 6-8 mm in diameter) for development into 12-15 mm hierarchical follicles (usually F6 follicles), which is an important process affecting laying performance in the poultry industry. Although transcriptomic analysis of chicken ovarian follicles has been reported, integrated analysis of chicken follicles for selection by using both transcriptomic and proteomic approaches is still rarely performed. In this study, we compared the proteomes and transcriptomes of SY and F6 follicles in laying hens and identified several genes involved in chicken follicle selection. Results: Transcriptomic analysis revealed 855 differentially expressed genes (DEGs) between SY follicles and F6 follicles in laying hens, among which 202 were upregulated and 653 were downregulated. Proteomic analysis revealed 259 differentially expressed proteins (DEPs), including 175 upregulated and 84 downregulated proteins. Among the identified DEGs and DEPs, changes in the expression of seven genes, including VLDLR1, WIF1, NGFR, AMH, BMP15, GDF6 and MMP13 , and nine proteins, including VLDLR, VTG1, VTG3, PSCA, APOB, APOV1, F10, ZP2 and ZP3L2, were validated. Further analysis indicated that the mRNA level of chicken VLDLR was higher in F6 follicles than in SY follicles and was also higher in granulosa cells (GCs) than in thecal cells (TCs), and it was stimulated by FSH in GCs of prehierarchical follicles. Conclusions: By comparing the proteomes and transcriptomes of SY and F6 follicles in laying hens, we identified several differentially expressed proteins/genes that might play certain roles in chicken follicle selection. These data may contribute to the identification of functional genes and proteins involved in chicken follicle selection.

scholarly journals Oviposition time, flock age, and egg position in clutch in relation to brown eggshell color in laying hens

2016 ◽  
Vol 95 (9) ◽  
pp. 2052-2057 ◽  
Author(s):  
S. Samiullah ◽  
J. Roberts ◽  
K. Chousalkar
Keyword(s):  
Laying Hens ◽  
Eggshell Color
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