scholarly journals In Sacco Evaluation of Rumen Protein Degradation Characteristics and In Vitro Enzyme Digestibility of Dry Roasted Whole Lupin Seeds (Lupinus albus)

1999 ◽  
Vol 12 (3) ◽  
pp. 358-365 ◽  
Author(s):  
P. Yu ◽  
A. R. Egan ◽  
B. J. Leury
Keyword(s):  
Protein Degradation ◽  
Lupinus Albus ◽  
Lupin Seeds ◽  
In Sacco ◽  
Enzyme Digestibility

scholarly journals Effect of extruding on ruminal disappearance and lower gastrointestinal tract digestion of white lupin seeds

1993 ◽  
Vol 73 (3) ◽  
pp. 571-579 ◽  
Author(s):  
A. R. Kibelolaud ◽  
M. Vernay ◽  
C. Bayourthe ◽  
R. Moncoulon
Keyword(s):  
Gastrointestinal Tract ◽  
Crude Protein ◽  
Lupinus Albus ◽  
Neutral Detergent Fiber ◽  
White Lupin ◽  
Lower Gastrointestinal Tract ◽  
Rumen Degradation ◽  
Outflow Rate ◽  
Lupin Seeds

The effect of extruding white lupin (Lupinus albus 'Lublanc') seeds (WLS) at 110, 130, 150 or 180 °C on the in vitro solubility of crude protein (CP) and in sacco rumen degradation and intestinal digestion of rumen escape CP and fiber was determined. Rumen degradation was estimated by incubating nylon bags in the rumen of cows for 2, 4, 8, 16, 24 and 48 h. Extruding WLS at 110, 130, 150 and 180 °C reduced the CP-solubility by 32.8, 47.7, 58.4 and 67.5%, respectively. The effective ruminal degradabilities of CP, acid detergent fiber (ADF) and neutral detergent fiber (NDF) were evaluated assuming a ruminal outflow rate of 0.06 h−1. Heating WLS at 110, 130, 150 and 180 °C decreased the ruminal degradability of CP value: 89.8, 79.9, 65.1, 61.8 vs. 93.4% (raw), respectively; the corresponding values for ADF and NDF were: 38.4, 35.3, 34.2, 27.6 vs. 43.6% (raw) and 37.7, 33.1, 32.2, 26.5 vs. 39.4% (raw). Postruminal digestion was estimated using a sequence of ruminal in situ incubation for 4, 8 and 16 h, in vitro incubation in an acid-pepsin bath for 3 h and a mobile nylon bag technique distal to the abomasum. Extrusion of WLS decreased the degradability of CP, ADF and NDF in the rumen with a corresponding increase in the amounts digested in the postruminal sections; the whole-tract digestibility was generally unchanged. Therefore, the processing shifted the digestion of these components from rumen to the lower gastrointestinal tract. Key words: White lupin seed, in vitro solubility, rumen degradability, postruminal digestion, crude protein, detergent fiber

In vitro fermentation of lupin seeds (Lupinus albus) and broad beans (Vicia faba): dynamic modulation of the intestinal microbiota and metabolomic output

2015 ◽  
Vol 6 (10) ◽  
pp. 3316-3322 ◽  
Author(s):  
Patricia Gullón ◽  
Beatriz Gullón ◽  
Freni Tavaria ◽  
Marta Vasconcelos ◽  
Ana Maria Gomes
Keyword(s):  
Vicia Faba ◽  
Intestinal Microbiota ◽  
Lupinus Albus ◽  
Human Gut ◽  
In Vitro Fermentation ◽  
Dynamic Modulation ◽  
Broad Beans ◽  
Wide Range ◽  
Lupin Seeds

Broad beans (Vicia faba) and lupin seeds (Lupinus albus) are legumes rich in a wide range of compounds, which may represent a useful dietary approach for modulating the human gut microbiome.

scholarly journals Untargeted Phytochemical Profile, Antioxidant Capacity and Enzyme Inhibitory Activity of Cultivated and Wild Lupin Seeds from Tunisia

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3452
Author(s):  
Amna Ben Hassine ◽  
Gabriele Rocchetti ◽  
Leilei Zhang ◽  
Biancamaria Senizza ◽  
Gökhan Zengin ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging Activity ◽  
Lupinus Albus ◽  
Radical Scavenging ◽  
Northern Region ◽  
Lupinus Luteus ◽  
Antioxidant Compounds ◽  
Lupin Seeds ◽  
Phytochemical Profile

Lupin seeds can represent a valuable source of phenolics and other antioxidant compounds. In this work, a comprehensive analysis of the phytochemical profile was performed on seeds from three Lupinus species, including one cultivar (Lupinus albus) and two wild accessions (Lupinus cossentinii and Lupinus luteus), collected from the northern region of Tunisia. Untargeted metabolomic profiling allowed to identify 249 compounds, with a great abundance of phenolics and alkaloids. In this regard, the species L. cossentinii showed the highest phenolic content, being 6.54 mg/g DW, followed by L. luteus (1.60 mg/g DW) and L. albus (1.14 mg/g DW). The in vitro antioxidant capacity measured by the ABTS assay on seed extracts ranged from 4.67 to 17.58 mg trolox equivalents (TE)/g, recording the highest values for L. albus and the lowest for L. luteus. The DPPH radical scavenging activity ranged from 0.39 to 3.50 mg TE/g. FRAP values varied between 4.11 and 5.75 mg TE/g. CUPRAC values for lupin seeds ranged from 7.20 to 8.95 mg TE/g, recording the highest for L. cossentinii. The results of phosphomolybdenum assay and metal chelation showed similarity between the three species of Lupinus. The acetylcholinesterase (AChE) inhibition activity was detected in each methanolic extract analyzed with similar results. Regarding the butyrylcholinesterase (BChE) enzyme, it was weakly inhibited by the Lupinus extracts; in particular, the highest activity values were recorded for L. albus (1.74 mg GALAE/g). Overall, our results showed that L. cossentinii was the most abundant source of polyphenols, consisting mainly in tyrosol equivalents (5.82 mg/g DW). Finally, significant correlations were outlined between the phenolic compounds and the in vitro biological activity measured, particularly when considering flavones, phenolic acids and lower-molecular-weight phenolics.

scholarly journals DDRE-09. DEVELOPING IDO-PROTACS TO IMPROVE IMMUNOTHERAPEUTIC EFFICACY IN PATIENTS WITH GLIOBLASTOMA

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii63-ii63
Author(s):  
Lakshmi Bollu ◽  
Derek Wainwright ◽  
Lijie Zhai ◽  
Erik Ladomersky ◽  
Kristen Lauing ◽  
...  
Keyword(s):  
Protein Degradation ◽  
Time Course ◽  
Immune Cell ◽  
Enzyme Inhibitors ◽  
Tumor Development ◽  
Ubiquitin Proteasome System ◽  
Degradation Pathway ◽  
Specific Protein ◽  
Cell Functions

Abstract INTRODUCTION Indoleamine 2,3-dioxygenase 1 (IDO; IDO1) is a rate-limiting enzyme that metabolizes the essential amino acid tryptophan into kynurenine. Recent work by our group has revealed that IDO promotes tumor development and suppresses immune cell functions independent of its enzyme activity. Moreover, pharmacologic IDO enzyme inhibitors that currently serve as the only class of drugs available for targeting immunosuppressive IDO activity, fail to improve the survival of patients with GBM. Here, we developed IDO-Proteolysis Targeting Chimeras (IDO-PROTACs). PROTACs bind to a specific protein and recruit an E3 ubiquitin ligase that enhance proteasome-mediated degradation of the target protein. METHODS A library of ≥100 IDO-PROTACs were developed by joining BMS986205 (IDO binder) with a linker group to various E3-ligase ligands. Western blot analysis of PROTAC-induced IDO degradation was tested in vitro among multiple human and mouse GBM cell lines including U87, GBM6, GBM43 and GL261 along a time course ranging between 1–96 hours of treatment and at varying concentrations. The mechanism of IDO protein degradation was investigated using pharmacologic ligands that inhibit or compete with the proteasome-mediated protein degradation pathway. RESULTS Primary screening identified several IDO-PROTACs with IDO protein degradation potential. Secondary screening showed that our lead compound has a DC50 value of ~0.5µM with an ability to degrade IDO in all GBM cells analyzed, and an initial activity within 12 hours of treatment that extended for up to 96 hours. Mutating the CRBN-binding ligand, pretreatment with the ubiquitin proteasome system inhibitors MG132 or MLN4924 or using unmodified parental compound all inhibited IDO protein degradation. CONCLUSIONS This study developed an initial IDO-PROTAC technology that upon further optimization, can neutralize both IDO enzyme and non-enzyme immunosuppressive effects. When combined with other forms of immunotherapy, IDO-PROTACs have the potential to substantially enhance immunotherapeutic efficacy in patients with GBM.

Potential of enzymes or a centrifuged rumen fluid to estimate proteolysis of foods

1998 ◽  
Vol 22 ◽  
pp. 120-122
Author(s):  
A. S. Chaudhry
Keyword(s):  
Rumen Fluid ◽  
Food Proteins ◽  
The Past ◽  
Ruminal Digestion ◽  
Selection Of ◽  
In Sacco

The need to develop an in vitro method to simulate ruminal digestion of protein foods has long been recognized. An in vitro method must be more rapid, consistent and convenient than in sacco methods. Purified enzymes have been examined in the past to estimate in vitro degradability of protein foods (Poos-Floyd et al., 1985; Aufrere et al., 1991; Luchini et al., 1996) or their fractions (Chaudhry and Webster, 1994). However, the selection of an appropriate enzyme for a range of foodstuffs remains to be realized. This study examined the potential of two enzymes and a centrifuged rumen fluid (CRF) to estimate proteolysis of food proteins.

Turnover of total proteins and ornithine aminotransferase during liver regeneration in rats

1980 ◽  
Vol 238 (1) ◽  
pp. E46-E52
Author(s):  
S. L. Augustine ◽  
R. W. Swick
Keyword(s):  
Amino Acids ◽  
Liver Regeneration ◽  
Protein Degradation ◽  
Partial Hepatectomy ◽  
Free Amino ◽  
Liver Protein ◽  
Ornithine Aminotransferase ◽  
Fractional Rate

The recovery of approximately 40% of the total liver protein during the first day after partial hepatectomy was shown to be due to the near cessation of protein breakdown rather than to an increase in protein synthesis. The decrease in degradation of total protein was less if rats were adrenalectomized or protein-depleted prior to partial hepatectomy. The effect of these treatments originally suggested that changes in free amino acid levels in liver might be related to the rate of protein degradation. However, no correlation was found between levels of total free amino acids and rates of breakdown. Measurements of individual amino acids during liver regeneration suggested that levels of free methionine and phenylalanine, amino acids that have been found to lower rates of protein degradation in vitro, are not correlated with rates of breakdown in vivo. The difference between the fractional rate of ornithine aminotransferase degradation (0.68/day and 0.28/day in sham-hepatectomized and partially hepatectomized rats, respectively) was sufficient to account for the higher level of this protein 3 days after surgery in the latter group.

scholarly journals Effect of pH on Fermentation Characteristics and Protein Degradation by Rumen Microorganisms In Vitro

1982 ◽  
Vol 65 (8) ◽  
pp. 1457-1464 ◽  
Author(s):  
J.D. Erfle ◽  
R.J. Boila ◽  
R.M. Teather ◽  
S. Mahadevan ◽  
F.D. Sauer
Keyword(s):  
Protein Degradation ◽  
Rumen Microorganisms ◽  
Effect Of Ph

The need to complement in vitro gas production measurements with residue determinations from in sacco degradabilities to improve the prediction of voluntary intake of hays

1997 ◽  
Vol 64 (1) ◽  
pp. 71-75 ◽  
Author(s):  
M. Blümmel ◽  
P. Bullerdieck
Keyword(s):  
Volume Ratio ◽  
Gas Production ◽  
Published Data ◽  
Fatty Acid Production ◽  
Residue Determination ◽  
In Vitro Gas Production ◽  
Gas Volume ◽  
In Sacco Degradability ◽  
In Sacco

AbstractThe need to complement in vitro gas production measurements with residue determination is demonstrated by the recalculation and reassessment of published data on in vitro gas production, in sacco degradabilities and voluntary dry matter intake (DMI). The in sacco degradability — gas volume ratio was determined at 24 and 48 h of incubation, termed partitioning factor (PF) and combined with rate and extent parameters of in sacco degradability and in vitro gas production to predict DMI. In vitro gas production and in sacco degradability characteristics (a + b) and c as described by the equation y = a + b(1−ect) explained 0·373 and 0·668 respectively of the variation in DMI of 19 legume and grass hays. The complementation of gas production parameters by the PF24 increased the R2 value to 0·744 with PF24 accounting for 0·407 of the variation in DMI, the rate of gas production (c) for 0·218 and the extent of gas production (a + b) for 0·119 of the variation in DMI. As a single parameter, PF48 showed the highest correlation (R2 = 0·597) with DMI but the combination of PF4S with rate and extent of in sacco or in vitro gas production measurements did not improve the correlation further, probably due to an intercorrelation between rates of fermentation and PF4S. Hays which were degraded at faster rates had higher PF values indicating proportionally higher microbial yield and lower short-chain fatty acid production per unit substrate degraded. Generally, hays with high in sacco degradabilities but proportionally low gas production i.e. hays with high PF values showed higher DMI.

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