scholarly journals Differential characterization of myogenic satellite cells with linolenic and retinoic acid in the presence of thiazolidinediones from prepubertal Korean black goats

2018 ◽  
Vol 31 (3) ◽  
pp. 439-448
Author(s):  
S. Subi ◽  
S. J. Lee ◽  
S. Shiwani ◽  
N. K. Singh
Keyword(s):  
Retinoic Acid ◽  
Satellite Cells ◽  
Myogenic Satellite Cells

Isolation and characterization of myogenic satellite cells from the muscular dystrophic hamster

2000 ◽  
Vol 32 (3) ◽  
pp. 257-265 ◽  
Author(s):  
D.C. McFarland ◽  
Y.N. Singh ◽  
A.D. Johnson ◽  
J.E. Pesall ◽  
K.K. Gilkerson ◽  
...  
Keyword(s):  
Satellite Cells ◽  
Isolation And Characterization ◽  
Myogenic Satellite Cells

Presenting White Blood Cell Count and Kinetics of Molecular Remission Predict Prognosis in Acute Promyelocytic Leukemia Treated With All-Trans Retinoic Acid: Result of the Randomized MRC Trial

Blood ◽  
1999 ◽  
Vol 93 (12) ◽  
pp. 4131-4143 ◽  
Author(s):  
Alan K. Burnett ◽  
David Grimwade ◽  
Ellen Solomon ◽  
Keith Wheatley ◽  
Anthony H. Goldstone
Keyword(s):  
Retinoic Acid ◽  
Promyelocytic Leukemia ◽  
Rt Pcr ◽  
Blood Cell Count ◽  
Relapse Risk ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid ◽  
To Receive ◽  
Risk Of Relapse

Abstract All-trans retinoic acid (ATRA) is an essential component of the treatment of acute promyelocytic leukemia (APL), but the optimal timing and duration remain to be determined. Molecular characterization of this disease can refine the diagnosis and could be potentially useful in monitoring response to treatment. Patients defined morphologically to have APL were randomized to receive a 5-day course of ATRA before commencing chemotherapy or to receive daily ATRA commencing with chemotherapy and continuing until complete remission (CR). The chemotherapy was that used in current MRC Leukaemia Trials. Outcome comparisons were by intention to treat with additional analysis for relevant risk factors. Patients were characterized by molecular techniques for the fusion products of the t(15;17) and monitored by reverse transcriptase-polymerase chain reaction (RT-PCR) during and after treatment. Two hundred thirty-nine patients were randomized. Treatment with extended ATRA resulted in a superior remission rate (87% v 70%, P < .001), due to fewer early and induction deaths (12% v 23%, P = .02), and less resistant disease (2% v 7%, P = .03), which was associated with a significantly more rapid recovery of neutrophils and platelets. Extended ATRA reduced relapse risk (20%v 36% at 4 years, P = .04) and resulted in superior survival (71% v 52% at 4 years, P = .005). Presenting white blood cell count (WBC) was a key determinant of outcome. The 70% of patients who presented with a WBC less than 10 × 109/L had a better CR (85% v62%, P = .0001) and reduced relapse risk (22% v42%, P = .002) and superior survival (69%v 43%, P < .0001). Within the low count group, extended ATRA resulted in a better CR (94% v 76%, P= .001), reduced relapse risk (13% v 35%, P = .04), and improved survival (80% v 57%, P = .0009). There was no evidence of benefit in patients presenting with a higher WBC (>10 × 109/L). Molecular monitoring after the third chemotherapy course had a correlation with risk of relapse. The relapse risk was 57% if the RT-PCR was positive versus 27% if the RT-PCR was negative (P = .006). APL patients who present with a low WBC derive substantial benefit from combining ATRA with induction chemotherapy until remission is achieved, whereas patients with a higher WBC did not benefit. Molecular characterization of disease can improve diagnostic precision and a positive RT-PCR after consolidation identifies patients at a higher risk of relapse.

scholarly journals Bovine Satellite Cells Isolated after 2 and 5 Days of Tissue Storage Maintain the Proliferative and Myogenic Capacity Needed for Cultured Meat Production

2021 ◽  
Vol 22 (16) ◽  
pp. 8376
Author(s):  
Stig Skrivergaard ◽  
Martin Krøyer Rasmussen ◽  
Margrethe Therkildsen ◽  
Jette Feveile Young
Keyword(s):  
Satellite Cells ◽  
Myogenic Differentiation ◽  
Expression Patterns ◽  
Meat Production ◽  
Positive Trend ◽  
Tissue Storage ◽  
Myogenic Potential ◽  
Cultured Meat ◽  
Start Up ◽  
Myogenic Satellite Cells

Cultured meat is an emerging alternative food technology which aims to deliver a more ethical, sustainable, and healthy muscle-tissue-derived food item compared to conventional meat. As start-up companies are rapidly forming and accelerating this technology, many aspects of this multi-faceted science have still not been investigated in academia. In this study, we investigated if bovine satellite cells with the ability to proliferate and undergo myogenic differentiation could be isolated after extended tissue storage, for the purpose of increasing the practicality for cultured meat production. Proliferation of bovine satellite cells isolated on the day of arrival or after 2 and 5 days of tissue storage were analyzed by metabolic and DNA-based assays, while their myogenic characteristics were investigated using RT-qPCR and immunofluorescence. Extended tissue storage up to 5 days did not negatively affect proliferation nor the ability to undergo fusion and create myosin heavy chain-positive myotubes. The expression patterns of myogenic and muscle-specific genes were also not affected after tissue storage. In fact, the data indicated a positive trend in terms of myogenic potential after tissue storage, although it was non-significant. These results suggest that the timeframe of which viable myogenic satellite cells can be isolated and used for cultured meat production can be greatly extended by proper tissue storage.

Preparation and characterization of micelles of oligomeric chitosan linked to all-trans retinoic acid

2012 ◽  
Vol 87 (2) ◽  
pp. 1176-1184 ◽  
Author(s):  
Ali Fattahi ◽  
Mohammad-Ali Golozar ◽  
Jaleh Varshosaz ◽  
Hamid Mirmohammad Sadeghi ◽  
Mohammadhossein Fathi
Keyword(s):  
Retinoic Acid ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid

Tissue-Engineered External Anal Sphincter Using Autologous Myogenic Satellite Cells and Extracellular Matrix: Functional and Histological Studies

2015 ◽  
Vol 44 (5) ◽  
pp. 1773-1784 ◽  
Author(s):  
Abdol-Mohammad Kajbafzadeh ◽  
Majid Kajbafzadeh ◽  
Shabnam Sabetkish ◽  
Nastaran Sabetkish ◽  
Seyyed Mohammad Tavangar
Keyword(s):  
Extracellular Matrix ◽  
Anal Sphincter ◽  
Satellite Cells ◽  
External Anal Sphincter ◽  
Histological Studies ◽  
Myogenic Satellite Cells

scholarly journals Clonal Characterization of Rat Muscle Satellite Cells: Proliferation, Metabolism and Differentiation Define an Intrinsic Heterogeneity

PLoS ONE ◽  
2010 ◽  
Vol 5 (1) ◽  
pp. e8523 ◽  
Author(s):  
Carlo A. Rossi ◽  
Michela Pozzobon ◽  
Andrea Ditadi ◽  
Karolina Archacka ◽  
Annalisa Gastaldello ◽  
...  
Keyword(s):  
Satellite Cells ◽  
Muscle Satellite Cells ◽  
Rat Muscle
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