scholarly journals Characteristics of Wet Distillers Grains on In vitro Ruminal Fermentation and Its Effects on Performance and Carcass Characteristics of Finishing Hanwoo Steers

2016 ◽  
Vol 29 (4) ◽  
pp. 530-538 ◽  
Author(s):  
Gyu Chul Ahn ◽  
Hyung Jun Kwak ◽  
Young Kyoon Oh ◽  
Yoo Kyung Lee ◽  
Sun Sik Jang ◽  
...  
Keyword(s):  
Carcass Characteristics ◽  
Distillers Grains ◽  
Ruminal Fermentation ◽  
Wet Distillers Grains

scholarly journals PSIX-1 Associative effect of wet-distillers’ grains plus solubles and tannin-rich peanut skin supplementation on in vitro rumen fermentation, greenhouse gas emissions, and microbiome changes

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 392-393
Author(s):  
Byeng Ryel Min ◽  
Lana Castleberry ◽  
David Parker ◽  
Heidi Waldrip ◽  
David Brauer ◽  
...  
Keyword(s):  
Greenhouse Gas ◽  
Rumen Fluid ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Distillers Grains ◽  
Strongly Correlated ◽  
Ruminal Fermentation ◽  
Peanut Skin ◽  
Wet Distillers Grains

Abstract The role of tannin-rich peanut skin (PS) and associative effects of different levels of wet distillers’ grains plus solubles (WDGS) on ruminal fermentation, microbial changes, and mitigation of greenhouse gas (GHG) and other emissions in bovine rumen fluid were investigated. All gases were collected using an Ankom in vitro system for methane (CH4), nitrous oxide (N2O), and hydrogen sulfide (H2S) analyses. Fifteen % ground PS against 0, 10, 20, 30, and 40 % DM of WDGS were used. RT-qPCR were conducted to determine microbial diversity. In the absence of PS, total CH4 and H2S, or CH4 and H2S productions per gram of DM substrate, were linearly increased (P < 0.05) with increasing WDGS. However, in the presence of PS, those trends were reversed and CH4 and H2S productions were decreased (P < 0.05), suggesting that a diet with 15% PS and supplementation of 10 and 20% WDGS were able to reduce CH4 and H2S emissions by 12 and 33%, respectively. In the presence of PS, rumen fermentation rate (as a measured by VFA) and acetate/propionate (A/P) ratio was decreased with increasing WDGS, with PS x WDGS interactions (P < 0.01). In the presence of PS, there was a decreased (P < 0.05) the average population of Bacteroidetes, total methanogens, Methanobrevibacter sp. AbM4, and total protozoa populations at 40% WDGS, with PS x WDGS interactions (P < 0.01). The population of total methanogens (R2 = 0.57; P < 0.01), Firmicutes populations (R2= 0.46: P < 0.05), and F/B ratio (R2 = 0.46; P < 0.03) were strongly correlated with ruminal methane gas production. Therefore, associative effect of tannin-rich PS and WDGS suppressed methanogenesis pathways directly across their antimethanogenic activity and secondarily throughout their modification of protozoa population.

Impact of a mixed culture of Lactobacillus casei and L. lactis on in vitro ruminal fermentation and the growth of feedlot steers fed barley-based diets

2009 ◽  
Vol 89 (2) ◽  
pp. 263-271 ◽  
Author(s):  
J. Baah ◽  
Y. Wang ◽  
T A McAllister
Keyword(s):  
Growth Performance ◽  
Mixed Culture ◽  
Lactobacillus Casei ◽  
Carcass Characteristics ◽  
Barley Grain ◽  
Feedlot Cattle ◽  
Ruminal Fermentation ◽  
Feedlot Steers ◽  
Barley Silage

The effect of a direct-fed microbial (DFM) poduct containing a mixed culture of Lactobacillus casei and L. lactis on in vitro ruminal fermentation of barley-grain/barley-silage-based backgrounding and finishing diets and on growth performance and carcass characteristics of feedlot cattle was evaluated during backgrounding (84 d) and finishing (140 d) of 100 Hereford × Angus steers (initial body weight = 280 ± 15.5 kg). The inclusion rates of DFM in the in vitro study were 4, 8, 12 and 16 million colony forming units (CFU) of lactic acid bacteria (LAB) kg-1 DM of substrate. Total in vitro volatile fatty acids (VFA) production increased at 6 and 12 h of incubation (P < 0.01; linear response) when the backgrounding diet was supplemented with DFM. Dry matter digestibility and VFA production also increased (P < 0.05) during a 12-h fermentation of the finishing diet. Steers were randomly allocated to one of four dietary treatments that comprised feeding DFM tp provide 0 (control), 4 × 107, 8 × 107, or 12 × 107 CFU kg-1 diet DM. Average daily gain (ADG, kg) and feed efficiency (G:F; kg gain kg-1 DM consumed) of steers improved (P = 0.002 and 0.001, respectively) as a result of feeding DFM during the backgrounding period, but not during the finishing period. Saleable meat and rib eye area decreased (P = 0.038, linear; and P = 0.041, quadratic) with DFM supplementation. The results indicated that supplementing barley-grain/barley-silage-based feedlot cattle diets with 12 × 107 CFU of the mixed culture of lactobacilli used in these studies could improve ruminal fermentation, ADG and G:F in backgrounding feedlot steers. However, supplementation during the finishing period may not be warranted in terms of growth performance and carcass characteristics. Differences in the microbial ecology of the intestinal tract as a result of differences in diet composition may account for the varied response between the backgrounding and finishing periods.Key words: Beef cattle, direct-fed microbial, growth performance, Lactobacillus casei, Lactobacillus lactis, ruminal fermentation

scholarly journals PSXII-7 In vitro ruminal and total tract digestibility of diets containing high inclusions of wet distillers grains for feedlot sheep

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 416-417
Author(s):  
Marco Túlio C Almeida ◽  
João Pedro A Bertoco ◽  
Maria Carolina G Arruda ◽  
Sérgio A G Pereira-Junior ◽  
Antonio A Cruz ◽  
...  
Keyword(s):  
Sugarcane Bagasse ◽  
Soybean Meal ◽  
Distillers Grains ◽  
In Vitro Digestibility ◽  
Wet Distillers Grains ◽  
The Difference ◽  
Quadratic Effects ◽  
Male Sheep

Abstract The objective was to evaluate the effects of increasing inclusion of wet distillers grains (WDG) on in vitro ruminal and total tract digestibility in feedlot sheep. Three crossbred ruminally-cannulated male sheep were used as rumen liquid donor. Treatments consisted of isonitrogenous and isoenergetic diets, containing 0, 15, 30 or 45% WDG on DM basis (Control, 15WDG, 30WDG, and 45WDG, respectively). Diets were composed of sugarcane bagasse (20%) and the concentrate (80%) composed of soybean meal (except 45WDG), wheat meal, WDG (except Control), urea, limestone, and mineral-vitamin premix. In vitro digestibility of DM (IVDMD), NDF (IVNDFD), and CP (IVCPD) were performed using the Ankom-DaisyII fermenter. Ruminal digestibility was evaluated after 48h incubation (39°C) using twenty-five filter bags. After 48h, twelve filter bags remained into each jar to evaluate the total tract digestibility. For it, 40mL of 6N HCl and 8g of pepsin were added and incubated for another 24h. The post-ruminal digestibility was calculated by the difference between rumen and total tract digestibility. Data were analyzed using SAS software. Contrasts were used to determine the linear and quadratic effects of WDG inclusions, as well as the contrast Control × WDG treatments. The WDG inclusion linearly decreased the ruminal and total DM and CP digestibility and increased the post-rumen CP digestibility (P &lt; 0.05). When all treatments with WDG were evaluated against the Control, a significant effect was observed for IVDMD, with averages of 68.86 × 77.38%, 5.60 × 0.77% and 71.41 × 78.20%, and for IVCPD, with averages of 68.89 × 95.27%, 21.30 × 1.99% and 90.15 × 97.27%, respectively for rumen, post-rumen and total tract digestibility (P &lt; 0.05). The results indicate that inclusions of WDG at up to 45% in the diets for sheep reduce the ruminal and total DM utilization, however, promote a better post-rumen use of CP of diets.

scholarly journals 337 Effect of adding urea to finishing diets containing two different inclusions of distillers grains on steer performance and carcass characteristics

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 134-135
Author(s):  
Bradley M Boyd ◽  
James C MacDonald ◽  
Matthew Luebbe ◽  
Galen E Erickson
Keyword(s):  
Fixed Effects ◽  
Dry Matter ◽  
Carcass Characteristics ◽  
Distillers Grains ◽  
Beef Steers ◽  
Performance Measurements ◽  
Minimal Impact ◽  
Wet Distillers Grains ◽  
Improved Performance ◽  
Urea Inclusion

Abstract Crossbred beef steers (n = 384) were utilized to evaluate the effects of adding urea to a dry rolled corn based finishing diet containing low inclusions of wet distillers grains plus solubles (WDGS). Treatments were set up as a 2 × 4 factorial arrangement with factors consisting of WDGS inclusion at either 12 or 20% of diet dry matter (DM) and urea inclusion at either 0, 0.4, 0.8, 1.2% of diet DM. Cattle were blocked and stratified by initial body weight (BW) and assigned randomly to treatment. The heavy block of cattle were harvested after 160 d on feed (DOF) and the middle and light blocks of cattle were harvested after 179 DOF. The model include the fixed effects of weight block, WDGS inclusion, urea inclusion, and their interactions. There were no significant interactions (P > 0.12) observed between WDGS inclusion and urea inclusion in the diet; therefore, only main effects are discussed. Increasing inclusion of WDGS improved (P < 0.04) carcass adjusted ADG and G:F. Dry matter intake was reduced (P = 0.04) with increasing WDGS inclusions. Fat thickness was increased (P = 0.04) with increased WDGS inclusion; however, no other carcass characteristics were significantly affected by WDGS inclusion. Cubic effects of urea inclusion were observed (P < 0.05) for HCW, final BW, ADG, and DMI on a carcass adjusted and live BW basis; however, cubic effects with increasing urea inclusion are of minimal biological relevance. There were no linear or quadratic responses (P > 0.11) for increasing urea inclusion in the diet for performance measurements or carcass characteristics. Increasing WDGS inclusion in the diet improved performance. Added urea in the diet had minimal impact on performance, regardless of wet distillers grains plus solubles inclusion.

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