scholarly journals Identification and Antimicrobial Activity Detection of Lactic Acid Bacteria Isolated from Corn Stover Silage

2015 ◽  
Vol 28 (5) ◽  
pp. 620-631 ◽  
Author(s):  
Dongxia Li ◽  
Kuikui Ni ◽  
Huili Pang ◽  
Yanping Wang ◽  
Yimin Cai ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Corn Stover ◽  
Activity Detection ◽  
Corn Stover Silage

scholarly journals A lower cost method of preparing corn stover for Irpex lacteus treatment by ensiling with lactic acid bacteria

2020 ◽  
Vol 33 (8) ◽  
pp. 1273-1283
Author(s):  
Sasa Zuo ◽  
Di Jiang ◽  
Dongze Niu ◽  
Mingli Zheng ◽  
Ya Tao ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Corn Stover ◽  
Lignin Content ◽  
Early Stage ◽  
Gas Production ◽  
Irpex Lacteus ◽  
Corn Stover Silage ◽  
Silage Quality

Objective: This study investigated a method of preparing corn stover for Irpex lacteus (<i>I. lacteus</i>) treatment to improve its <i>in vitro</i> rumen degradability under non-sterile conditions.Methods: Corn stover was inoculated with <i>Lactobacillus plantarum</i> (<i>L. plantarum</i>), <i>Lactobacillus buchneri</i> (<i>L. buchneri</i>), and an equal mixture of these strains, and ensiled for 0, 3, 7, 14, and 28 days. After each period, a portion of the silage was sampled to assess the silage quality, and another portion of the silage was further treated with <i>I. lacteus</i> at 28°C for 28 d. All the samples were analyzed for fermentation quality, chemical composition, and <i>in vitro</i> gas production (IVGP) as a measure of rumen fermentation capacity.Results: Lactic acid bacteria (LAB) was found to improve the silage quality of the corn stover, and the corn stover silage inoculated with <i>L. plantarum</i> produced more lactic acid and higher IVGP than other silage groups. The <i>I. lacteus</i> colonies flourished in the early stage of corn stover silage, especially on the 3-d corn stover silage inoculated with both <i>L. plantarum</i> and <i>L. buchneri.</i> This led to an 18% decrease in the acid detergent lignin content, and a 49.6% increase in IVGP compared with the raw stover.Conclusion: The combination of ensiling with the mixed LAB inoculation and <i>I. lacteus</i> treatment provided a cost-effective method for the improvement of the IVGP of corn stover from 164.8 mL/g organic matter (OM) to 246.6 mL/g OM.

scholarly journals Selection of enhanced antimicrobial activity posing lactic acid bacteria characterised by (GTG)5-PCR fingerprinting

2014 ◽  
Vol 52 (7) ◽  
pp. 4124-4134 ◽  
Author(s):  
Joana Šalomskienė ◽  
Asta Abraitienė ◽  
Dovilė Jonkuvienė ◽  
Irena Mačionienė ◽  
Jūratė Repečkienė
Keyword(s):  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Pcr Fingerprinting ◽  
Selection Of

Cell-free supernatants produced by lactic acid bacteria reduce Salmonella population in vitro

Microbiology ◽  
2021 ◽  
Vol 167 (11) ◽  
Author(s):  
Alberto Gonçalves Evangelista ◽  
Jessica Audrey Feijó Corrêa ◽  
João Vitor Garcia dos Santos ◽  
Eduardo Henrique Custódio Matté ◽  
Mônica Moura Milek ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Antimicrobial Resistance ◽  
Lactic Acid Bacteria ◽  
Type Species ◽  
Feed Additives ◽  
Poultry Feed ◽  
Content Type ◽  
Link Type

The genus Salmonella is closely associated with foodborne outbreaks and animal diseases, and reports of antimicrobial resistance in Salmonella species are frequent. Several alternatives have been developed to control this pathogen, such as cell-free supernatants (CFS). Our objective here was to evaluate the use of lactic acid bacteria (LAB) CFS against Salmonella in vitro. Seventeen strains of LAB were used to produce CFS, and their antimicrobial activity was screened towards six strains of Salmonella . In addition, CFS were also pH-neutralized and/or boiled. Those with the best results were lyophilized. MICs of lyophilized CFS were 11.25–22.5 g l–1. Freeze-dried CFS were also used to supplement swine and poultry feed (11.25 g kg–1) and in vitro simulated digestion of both species was performed, with Salmonella contamination of 5×106 and 2×105 c.f.u. g−1 of swine and poultry feed, respectively. In the antimicrobial screening, all acidic CFS were able to inhibit the growth of Salmonella . After pH neutralization, Lactobacillus acidophilus Llorente, Limosilactobacillus fermentum CCT 1629, Lactiplantibacillus plantarum PUCPR44, Limosilactobacillus reuteri BioGaia, Lacticaseibacillus rhamnosus ATCC 7469 and Pediococcus pentosaceus UM116 CFS were the only strains that partially maintained their antimicrobial activity and, therefore, were chosen for lyophilization. In the simulated swine digestion, Salmonella counts were reduced ≥1.78 log c.f.u. g–1 in the digesta containing either of the CFS. In the chicken simulation, a significant reduction was obtained with all CFS used (average reduction of 0.59±0.01 log c.f.u. ml–1). In general, the lyophilized CFS of L. fermentum CCT 1629, L. rhamnosus ATCC 7469 and L. acidophilus Llorente presented better antimicrobial activity. In conclusion, CFS show potential as feed additives to control Salmonella in animal production and may be an alternative to the use of antibiotics, minimizing problems related to antimicrobial resistance.

ISOLATION, IDENTIFICATION AND ANTIMICROBIAL ACTIVITY OF LACTIC ACID BACTERIA FROM ALGERIAN HONEYBEE COLLECTED POLLEN

2010 ◽  
pp. 51-58 ◽  
Author(s):  
H. Belhadj ◽  
D. Harzallah ◽  
S. Khennouf ◽  
S. Dahamna ◽  
S. Bouharati ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria
Sign in / Sign up

Export Citation Format

Share Document