scholarly journals Immune Responses in Broiler Chicks Fed Propolis Extraction Residue-supplemented Diets

2014 ◽  
Vol 28 (1) ◽  
pp. 135-142 ◽  
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2012 ◽  
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Author(s):  
Gamal A Elmowalid ◽  
Marwa I Abd El-Hamid ◽  
Ashraf M Abd El-Wahab ◽  
Mohammad Atta ◽  
Gamal Abd El-Naser ◽  
...  

2013 ◽  
Vol 46 (2) ◽  
pp. 183-188 ◽  
Author(s):  
Xu Ting Li ◽  
Bin Wang ◽  
Jin Liang Li ◽  
Rui Yang ◽  
Si Cong Li ◽  
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2019 ◽  
Vol 10 (-) ◽  
Author(s):  
M. Shahryari ◽  
A. Emadinia ◽  
A. Davoodi ◽  
M. Omrani ◽  
M. Jafari ◽  
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Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2293
Author(s):  
Rujiu Hu ◽  
Haojing Liu ◽  
Mimi Wang ◽  
Jing Li ◽  
Hua Lin ◽  
...  

Avian pathogenic Escherichia coli (APEC) infection in poultry causes enormous economic losses and public health risks. Bacterial outer membrane vesicles (OMVs) and nano-sized proteolipids enriched with various immunogenic molecules have gained extensive interest as novel nanovaccines against bacterial infections. In this study, after the preparation of APEC O2-derived OMVs (APEC_OMVs) using the ultracentrifugation method and characterization of them using electron microscopy and nanoparticle tracking analyses, we examined the safety and vaccination effect of APEC_OMVs in broiler chicks and investigated the underlying immunological mechanism of protection. The results showed that APEC_OMVs had membrane-enclosed structures with an average diameter of 89 nm. Vaccination with 50 μg of APEC_OMVs had no side effects and efficiently protected chicks against homologous infection. APEC_OMVs could be effectively taken up by chicken macrophages and activated innate immune responses in macrophages in vitro. APEC_OMV vaccination significantly improved activities of serum non-specific immune factors, enhanced the specific antibody response and promoted the proliferation of splenic and peripheral blood lymphocytes in response to mitogen. Furthermore, APEC_OMVs also elicited a predominantly IFN-γ-mediated Th1 response in splenic lymphocytes. Our data revealed the involvement of both non-specific immune responses and specific antibody and cytokine responses in the APEC_OMV-mediated protection, providing broader knowledge for the development of multivalent APEC_OMV-based nanovaccine with high safety and efficacy in the future.


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