scholarly journals Isolation and Characterization of Cultured Chicken Oviduct Epithelial Cells and In Vitro Validation of Constructed Ovalbumin Promoter in these Cells

2020 ◽  
Author(s):  
Hyeon Yang ◽  
Bo Ram Lee ◽  
Hwi-Cheul Lee ◽  
Sun Keun Jung ◽  
Ji-Youn Kim ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Isolation And Characterization ◽  
Chicken Oviduct ◽  
Oviduct Epithelial Cells

scholarly journals Functional and Genomic Characterization of Ligilactobacillus salivarius TUCO-L2 Isolated From Lama glama Milk: A Promising Immunobiotic Strain to Combat Infections

2020 ◽  
Vol 11 ◽  
Author(s):  
Sandra Quilodrán-Vega ◽  
Leonardo Albarracin ◽  
Flavia Mansilla ◽  
Lorena Arce ◽  
Binghui Zhou ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Functional Characterization ◽  
Comparative Genomic ◽  
Cytokines And Chemokines ◽  
Isolation And Characterization ◽  
Intestinal Pathogens ◽  
Lama Glama

Potential probiotic or immunobiotic effects of lactic acid bacteria (LAB) isolated from the milk of the South American camelid llama (Lama glama) have not been reported in published studies. The aim of the present work was to isolate beneficial LAB from llama milk that can be used as potential probiotics active against bacterial pathogens. LAB strains were isolated from llama milk samples. In vitro functional characterization of the strains was performed by evaluating the resistance against gastrointestinal conditions and inhibition of the pathogen growth. Additionally, the adhesive and immunomodulatory properties of the strains were assessed. The functional studies were complemented with a comparative genomic evaluation and in vivo studies in mice. Ligilactobacillus salivarius TUCO-L2 showed enhanced probiotic/immunobiotic potential compared to that of other tested strains. The TUCO-L2 strain was resistant to pH and high bile salt concentrations and demonstrated antimicrobial activity against Gram-negative intestinal pathogens and adhesion to mucins and epithelial cells. L. salivarius TUCO-L2 modulated the innate immune response triggered by Toll-like receptor (TLR)-4 activation in intestinal epithelial cells. This effect involved differential regulation of the expression of inflammatory cytokines and chemokines mediated by the modulation of the negative regulators of the TLR signaling pathway. Moreover, the TUCO-L2 strain enhanced the resistance of mice to Salmonella infection. This is the first report on the isolation and characterization of a potential probiotic/immunobiotic strain from llama milk. The in vitro, in vivo, and in silico investigation performed in this study reveals several research directions that are needed to characterize the TUCO-L2 strain in detail to position this strain as a probiotic or immunobiotic that can be used against infections in humans or animals, including llama.

Isolation, culture, and characterization of equine oviduct epithelial cells in vitro

1995 ◽  
Vol 41 (4) ◽  
pp. 468-478 ◽  
Author(s):  
Philip G. A. Thomas ◽  
George G. Ignotz ◽  
Barry A. Ball ◽  
Paula G. Miller ◽  
Steven P. Brinsko ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Oviduct Epithelial Cells

In vitro adherence and invasion of primary chicken oviduct epithelial cells by Gallibacterium anatis

2017 ◽  
Vol 203 ◽  
pp. 136-142 ◽  
Author(s):  
Xiu-Ping Zhang ◽  
Cai-Jing Lu ◽  
Yong-Tao Li ◽  
Xia Yang ◽  
Xin-Wei Wang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Chicken Oviduct ◽  
Oviduct Epithelial Cells

scholarly journals Isolation and characterization of rat glomerular epithelial cells in vitro

1978 ◽  
Vol 14 (1) ◽  
pp. 21-30 ◽  
Author(s):  
Jeffrey I. Kreisberg ◽  
Richard L. Hoover ◽  
Morris J. Karnovsky
Keyword(s):  
Epithelial Cells ◽  
Isolation And Characterization ◽  
Glomerular Epithelial Cells

PENGEMBANGAN TEKNOLOGI SELEKSI IN VITRO BERULANG (REPEAT CYCLING–IN VITRO SELECTION) TOLERAN STRES KEKERINGAN UNTUK PERBAIKAN MUTU GENETIK DAN PRODUKSI TANAMAN KEDELAI BERMIKORIZA

2017 ◽  
Vol 1 (1) ◽  
pp. 74-84
Author(s):  
Ahmad Riduan ◽  
Rainiyati Rainiyati ◽  
Yulia Alia
Keyword(s):  
Arbuscular Mycorrhizae ◽  
In Vitro Selection ◽  
Soil Samples ◽  
Single Spore ◽  
Isolation And Characterization ◽  
Single Spore Culture ◽  
Spore Culture ◽  
Glomus Sp

Every plant rhizospheres in any ecosystem there are various living microorganisms including Arbuscular Mycorrhizae Fungi (AMF).  An isolation and characterization is required to investigate the species or type of the AMF. This research was aimed at studying the isolation and characterization of AMF sporulation in soybean rhizospheres in Jambi Province. The results of evaluation on soil samples before trapping showed that there are spores from three genus of AMF twelve types Glomus , two types Acaulospora and one type of Enthrophospora.  Following single spore culture in soybean rhizosphere, 5 spore types were obtained:  Glomus sp-1, Glomus sp-4, Glomus sp-7, Glomus sp-8 Glomus sp-10.

In vitro optimization of the Gallus domesticus oviduct epithelial cells culture

2012 ◽  
Vol 77 (9) ◽  
pp. 1834-1845 ◽  
Author(s):  
K. Kasperczyk ◽  
A. Bajek ◽  
R. Joachimiak ◽  
K. Walasik ◽  
A. Marszalek ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Gallus Domesticus ◽  
Oviduct Epithelial Cells

Arginine synthesis by hepatomas in vitro. II. Isolation and characterization of Morris hepatoma variants unable to convert ornithine to arginine, and modulation of urea-cycle enzymes by dexamethasone and cyclic-AMP

1984 ◽  
Vol 68 (1) ◽  
pp. 305-319
Author(s):  
S.J. Goss
Keyword(s):  
Cyclic Amp ◽  
Liver Cells ◽  
Isolation And Characterization ◽  
Rat Liver Cells ◽  
Morris Hepatoma ◽  
Ornithine Transcarbamoylase ◽  
Cellular Incorporation

‘77orn’, a derivative of the Morris rat hepatoma 7777, stably expresses high levels of ornithine transcarbamoylase (OTC) and carbamoylphosphate synthetase I (CPS-I), and is able to grow indefinitely in ornithine-medium (medium with ornithine in place of arginine). Variants that have lost this ability are isolated from 77orn by a ‘suicide’ selective technique dependent on the cellular incorporation of [3H]ornithine. These variants, which have reduced levels of CPS-I, or of both CPS-I and OTC, are shown to have developed multiple hormonal requirements; their enzyme deficiencies can be reversed by use of an appropriately supplemented medium. In particular, CPS-I is inducible by dexamethasone and dibutyryl-cyclic-AMP in combination. Cholera toxin can be used instead of cyclic-AMP, but then butyrate is additionally required if the induction is to be maintained in the long term. The use of these agents in excess can depress OTC. Several other hepatomas, and alos explanted foetal rat liver cells, have similar requirements for CPS-I expression. It is argued that multiple hormonal requirements for CPS-I production are normal in liver cells in vitro, and that hormone-independent hepatomas should be regarded as abnormal. The implications of this for the somatic cell genetic investigation of differentiation are briefly discussed.

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