scholarly journals Determination of Dynamic Ranges for Quantitative Analysis Using Electrospray Droplet Impact Ionization and Matrix-Assisted Laser Desorption Ionization

2009 ◽  
Vol 57 (2) ◽  
pp. 81-87 ◽  
Author(s):  
Daiki ASAKAWA ◽  
Yutaka HASHIMOTO ◽  
Kenzo HIRAOKA
Keyword(s):  
Quantitative Analysis ◽  
Impact Ionization ◽  
Laser Desorption ◽  
Droplet Impact ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Determination of 1,5‒anhydro‒glucitol–carrier protein conjugates by matrix‒assisted laser desorption/ionization tof mass spectrometry and antibody formation

2000 ◽  
Vol 14 (3) ◽  
pp. 85-92 ◽  
Author(s):  
Li-Jiang Xuan ◽  
Hiroyuki Tanaka ◽  
Satoshi Morimoto ◽  
Yukihiro Shoyama ◽  
Hiroshi Akanuma ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Serum Albumin ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Protein Conjugates ◽  
Desorption Ionization ◽  
Chicken Lysozyme ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

In order to prepare the monoclonal antibody against 1,5-anhydro-glucitol (1), it was conjugated with bovine serum albumin (BSA), human serum albumin (HSA) or chicken lysozyme (CL) using succinate orβ-alanine succinate as a spacer to produce individual antigen conjugates. The number of hapten contained in each antigen conjugate was determined by matrix-assisted laser desorption/ionization tof mass (MALDI-tof-MS) spectrometry. The formation of monoclonal antibody (MAb) was also discussed.

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