scholarly journals Micro-heterogeneities in Native and Recombinant Proteins Studied by Electrospray Ionization and Fast Atom Bombardment Mass Spectrometry Coupled with Micro-column Liquid Chromatography.

1995 ◽  
Vol 43 (6) ◽  
pp. 325-338
Author(s):  
Keiichiro ISHIKAWA ◽  
Jari TUOMINEN ◽  
Michio MURAKI ◽  
Hitoshi NAGAHORA ◽  
Yoshifumi JIGAMI ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Column Liquid Chromatography ◽  
Liquid Chromatography ◽  
Electrospray Ionization ◽  
Recombinant Proteins ◽  
Fast Atom Bombardment ◽  
Micro Column Liquid Chromatography

scholarly journals Column Liquid Chromatography/Electrospray Ionization-Time of Flight-Mass Spectrometry and Ultraperformance Column Liquid Chromatography/Mass Spectrometry Methods for the Determination of Ginkgolides and Bilobalide in the Leaves of Ginkgo biloba and Dietary Supplements

2009 ◽  
Vol 92 (2) ◽  
pp. 645-652 ◽  
Author(s):  
Bharathi Avula ◽  
Yan-Hong Wang ◽  
Troy J Smillie ◽  
Ikhlas A Khan
Keyword(s):  
Mass Spectrometry ◽  
Column Liquid Chromatography ◽  
Liquid Chromatography ◽  
Dietary Supplements ◽  
Electrospray Ionization ◽  
Ginkgo Biloba ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Abstract Column liquid chromatography/electrospray ionization-time of flight-mass spectrometry (LC/ESI-TOF-MS) and ultraperformance column liquid chromatographic/MS (UPLC/MS) methods were developed for the determination of 5 terpenelactones in the leaves of Ginkgo biloba. The separation was performed using a C18 column and a mobile phase composed of water and acetonitrile. The limit of detection (LOD) values for ginkgolide J, ginkgolide C, ginkgolide B, and ginkgolide A were found to be in the range of 110 ng/mL by both methods, and the bilobalide LOD was 200 ng/mL by LC/ESI-TOF and 35 ng/mL by UPLC/MS. Using the optimized method, 4 commercial products were analyzed. Concentrations of total marker compounds in these products were in the 0.0915.4 range, with individual ginkgo terpenelactones being detected at ppb levels. LC/MS with ESI is described for the identification and quantification of ginkgolides and bilobalide in plant samples and dietary supplements that claim to contain G. biloba. These methods involve the use of the M NH4<sup/> and M H<sup/> ions of sesquiterpenoids in the positive ion mode with extractive ion monitoring by LC/ESI-TOF-MS and selected ion monitoring by UPLC/MS. The LC method coupled with MS analysis yielded well-separated peaks which resulted in a clear identification of the compounds.

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