A New Method for the Sequence Determination of a Peptide Mixture through Molecular Weight Measurements by Mass Spectrometry

1980 ◽  
Vol 28 (2) ◽  
pp. 169-174 ◽  
Author(s):  
Takekiyo Matsuo ◽  
Itsuo Katakuse ◽  
Hisashi Matsuda ◽  
Yasutsugu Shimonishi ◽  
Yeong-Man Hong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
New Method ◽  
Sequence Determination ◽  
Peptide Mixture

A NEW METHOD FOR SEQUENCE DETERMINATION OF PEPTIDE MIXTURES BY EDMAN-DEGRADATION AND FIELD DESORPTION MASS SPECTROMETRY

1979 ◽  
Vol 8 (11) ◽  
pp. 1369-1372 ◽  
Author(s):  
Yasutsugu Shimonishi ◽  
Yeong-Man Hong ◽  
Takekiyo Matsuo ◽  
Itsuo Katakuse ◽  
Hisashi Matsuda
Keyword(s):  
Mass Spectrometry ◽  
New Method ◽  
Edman Degradation ◽  
Field Desorption ◽  
Sequence Determination ◽  
Desorption Mass Spectrometry ◽  
Peptide Mixtures ◽  
Field Desorption Mass Spectrometry

scholarly journals NEW METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF FEBUXOSTAT IN HUMAN PLASMA BY LIQUID CHROMATOGRAPHY–MASS SPECTROMETRY

2016 ◽  
Vol 8 (9) ◽  
pp. 61 ◽  
Author(s):  
Narottam Pal ◽  
Avanapu Srinivasa Rao ◽  
Pigilli Ravikumar
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Human Plasma ◽  
Method Development ◽  
Multiple Reaction Monitoring ◽  
Internal Standard ◽  
New Method ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

<p><strong>Objective</strong>:<strong> </strong>To develop a new method and validate the same for the determination of Febuxostat (FBS) in human plasma by liquid chromatography–mass spectrometry (LCMS).</p><p><strong>Methods</strong>:<strong> </strong>The present method utilized reversed-phase high-performance liquid chromatography with tandem mass spectroscopy. Febuxostat D9 (FBS D9) was used as internal standard (IS). The analyte and internal standard were separated from human plasma by using solid phase extraction method. Zorbax Eclipse XDB, C<sub>8</sub>, 100 mm x 4.6 mm, 3.5 µm column was used and HPLC grade acetonitrile, 5 millimolar (mM) ammonium format (80: 20, v/v) as mobile phase, detected by mass spectrometry operating in positive ion and multiple reaction monitoring modes.</p><p><strong>Results</strong>:<strong> </strong>The parent and production transitions for FBS and internal standard were at m/z 317.1→261.0 and 326.1→262.0 respectively. The method was validated for system suitability, specificity, carryover effect, linearity, precision, accuracy, matrix effect, sensitivity and stability. The linearity range was from 20.131 ng/ml to10015. 534 ng/ml with a correlation coefficient of 0.999. Precision results (%CV) across six quality control samples were within the limit. The percentage recovery of FBS and internal standard from matrix samples was found to be 76.57% and 75.03% respectively.</p><p><strong>Conclusion</strong>:<strong> </strong>Present study describes new LC-MS method for the quantification of FBS in a pharmaceutical formulation. According to validation results, it was found to be a simple, sensitive, accurate and precise method and also free from any kind of interference. Therefore the proposed analytical method can be used for routine analysis for the estimation of FBS in its formulation.</p>

A new method for sequence determination of large oligonucleotides

1964 ◽  
Vol 17 (4) ◽  
pp. 389-394 ◽  
Author(s):  
Robert W. Holley ◽  
James T. Madison ◽  
Ada Zamir
Keyword(s):  
New Method ◽  
Sequence Determination
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