scholarly journals Degradation Characteristics of A Novel Multi-Enzyme-Possessing Bacillus licheniformis TK3-Y Strain for the Treatment of High-Salinity Fish Wastes and Green Seaweeds

2015 ◽  
Vol 18 (4) ◽  
pp. 349-357 ◽  
Author(s):  
Kyeong Hwan Kang ◽  
Joong Kyun Kim
KSBB Journal ◽  
2020 ◽  
Vol 35 (4) ◽  
pp. 288-293
Author(s):  
Kyeong Hwan Kang ◽  
Heejin Lee ◽  
Taejin Park ◽  
Ji Han Sim ◽  
Seung-Young Kim

Author(s):  
B.K. Ghosh

Periplasm of bacteria is the space outside the permeability barrier of plasma membrane but enclosed by the cell wall. The contents of this special milieu exterior could be regulated by the plasma membrane from the internal, and by the cell wall from the external environment of the cell. Unlike the gram-negative organism, the presence of this space in gram-positive bacteria is still controversial because it cannot be clearly demonstrated. We have shown the importance of some periplasmic bodies in the secretion of penicillinase from Bacillus licheniformis.In negatively stained specimens prepared by a modified technique (Figs. 1 and 2), periplasmic space (PS) contained two kinds of structures: (i) fibrils (F, 100 Å) running perpendicular to the cell wall from the protoplast and (ii) an array of vesicles of various sizes (V), which seem to have evaginated from the protoplast.


Author(s):  
Xiaorong Zhu ◽  
Richard McVeigh ◽  
Bijan K. Ghosh

A mutant of Bacillus licheniformis 749/C, NM 105 exhibits some notable properties, e.g., arrest of alkaline phosphatase secretion and overexpression and hypersecretion of RS protein. Although RS is known to be widely distributed in many microbes, it is rarely found, with a few exceptions, in laboratory cultures of microorganisms. RS protein is a structural protein and has the unusual properties to form aggregate. This characteristic may have been responsible for the self assembly of RS into regular tetragonal structures. Another uncommon characteristic of RS is that enhanced synthesis and secretion which occurs when the cells cease to grow. Assembled RS protein with a tetragonal structure is not seen inside cells at any stage of cell growth including cells in the stationary phase of growth. Gel electrophoresis of the culture supernatant shows a very large amount of RS protein in the stationary culture of the B. licheniformis. It seems, Therefore, that the RS protein is cotranslationally secreted and self assembled on the envelope surface.


2013 ◽  
Author(s):  
Fan Zhang ◽  
Desheng Ma ◽  
Qiang Wang ◽  
Youyi Zhu ◽  
Wenli Luo

2018 ◽  
Vol 7 (3) ◽  
Author(s):  
Budiasih Wahyuntari., dkk

Isolate I-5 was isolated from Ciseeng hot spring, West Java and was identified as Bacillus licheniformis I-5. The isolate produces extracellular xylanolytic enzymes on Oatspelt containing Luria broth agar medium. Optimal activity of the crude enzyme was  observed at 50ºC and pH 7. The effect of sodium dodecyl sulphate, b-mercaptoethanol and Triton-X100 were observed. Incubating the crude enzyme in 1.5% SDS and 1.5% b-mercaptoethanol at 50oC for 90 minutes then adding Triton-X100 at final concentration of 3.5% for 45 minutes only reduced 5.75% of the initial enzyme activity. SDS/PAGE and zymogram analysis showed that at least two xylanolytic enzymes presence in the crude enzyme. The molecular weight of the enzyme was estimated about 127 and 20kD. The enzyme hydrolysed xylan into xylobiose, xylotriose and other longer xylooligosaccharides. Thermal stability of the crude enzyme was observed at 50, 60, and 70oC and pH 7 and 8. The results showed that the half time of the crude enzyme incubated at 50, 60, and 70oC pH 7 was 2 hours 55 minutes; 2 hours 33 minutes and 1 hour 15 minutes respectively. The half time at 50, 60 and 70oC, pH 8 was 2 hours 48 minutes; 1 hour 22 minutes and 1 hour 9 minutes respectively.keywords: Xilanase, Bacillus licheniformis I-5, thermal stability


Author(s):  
O. Merzlyakova ◽  
V. Rogachyev ◽  
V. Chegodaev

The efficiency of introducing probiotics based on strains of Bacillus subtilis, Bacillus licheniformis and their consortium in the amount of 150 g/t of feed into the diets of laying quails has been studied. The experiment lasting 182 days has been carried out on four groups of quails with 30 heads in each. The quails have been housed in the broiler battery in compliance with the required microclimate conditions. Quails of all groups have been received the main diet (compound feed) developed taking into account their age and physiological characteristics. The quails of the 1st, 2nd and 3rd experimental groups in addition to the main diet received probiotics (150 g/t compound feed) based on strains Bacillus subtilis, Bacillus licheniformis and their consortium, respectively. It has been found that feeding the laying quails of the consortium of strains Bacillus subtilis and Bacillus licheniformis had the most significant positive impact on their productive performance, it allowed to increase egg production by 7,81 %, egg laying intensity by 5,0 %, egg mass yield by 9,77 %, while reducing feed expenditures for 10 eggs by 13,35 %. The yield of hatching eggs has been increased by 7,03 %, hatchability of chickens from laid and fertilized eggs by 8,33 and 8,35 %, brooding waste decreased by 21,74 %. Hematological parameters of quails during the whole experiment were within the physiological norm. The economic effect calculated on the basis of data on the cost of compound feed, probiotics and the cost of sold eggs of quail laying was 14,56 % in the 3rd experimental group (in relation to the control group).


Author(s):  
Ekaterina Shchurova ◽  
Ekaterina Shchurova ◽  
Rimma Stanichnaya ◽  
Rimma Stanichnaya ◽  
Sergey Stanichny ◽  
...  

Sivash bay is the shallow-water lagoon of the Azov Sea. Restricted water exchange and high evaporation form Sivash as the basin with very high salinity. This factor leads to different from the Azov Sea thermal and ice regimes of Sivash. Maine aim of the study presented to investigate recent state and changes of the characteristics and processes in the basin using satellite data. Landsat scanners TM, ETM+, OLI, TIRS together with MODIS and AVHRR were used. Additionally NOMADS NOAA and MERRA meteorological data were analyzed. The next topics are discussed in the work: 1. Changes of the sea surface temperature, ice regime and relation with salinity. 2. Coastal line transformation – long term and seasonal, wind impact. 3. Manifestation of the Azov waters intrusions through the Arabat spit, preferable wind conditions.


2020 ◽  
Vol 16 (8) ◽  
pp. 1044-1057 ◽  
Author(s):  
Hamdoon A. Mohammed

Background: Suaeda is a halophytic genus belonging to the Amaranthaceae family and can survive in the high salted marsh areas of the world. Suaeda plants can biosynthesize natural substances with powerful antioxidant activity and are considered as a renewable source of energy, food, and edible oil for a larger number of populations living in the harsh environment with high salinity and drought conditions. These plants also meet folk and alternative medicines' needs. Methods: The review encompasses available scientific literature related to folk medicinal uses of Suaeda plants, their nutritional values, and chemical constituents. In addition, the biological trials applied for the Suaeda plants are also part of the review. The review covers the researches from major science literature search engines and other sites representing scientific literature, i.e., Scifinder, Google Scholar, PubMed, ScienceDirect, Scopus, and Google. The searches were programmed on the advance options available in the search engines and are latest up to November 2019. The searches were exhaustive and rechecked for accuracy. Conclusion: The study summarizes the uses of Suaeda plants as a remedy for various ailments due to their contents from the polyphenols and flavonoids. The comparatively large amounts of fixed oils, minerals, and vitamins in Suaeda plants have also made them potential renewable sources for foods.


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