scholarly journals Fractionation and Characterization of Fractions with High Antioxidative Activity from the Gelatin Hydrolysates of Korean Rockfish Sebastes schlegelii Skin

2011 ◽  
Vol 14 (3) ◽  
pp. 168-173
Author(s):  
Hyung-Jun Kim ◽  
Kwon-Hyun Park ◽  
Jun-Ho Shin ◽  
Ji-Sun Lee ◽  
Min-Soo Heu ◽  
...  
Keyword(s):  
Antioxidative Activity ◽  
Sebastes Schlegelii ◽  
Korean Rockfish

scholarly journals Physicochemical Properties, Cytotoxicity, and Antioxidative Activity of Natural Deep Eutectic Solvents Containing Organic Acid

2019 ◽  
Vol 33 (1) ◽  
pp. 1-18 ◽  
Author(s):  
Anamarija Mitar ◽  
Manuela Panić ◽  
Kristina Radošević ◽  
Ivana Radojčić Redovniković ◽  
Kristina Zagajski Kučan ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Antioxidative Activity ◽  
Deep Eutectic Solvents ◽  
Oxygen Radical ◽  
Structural Variations ◽  
Natural Deep Eutectic Solvents ◽  
Environmentally Safe ◽  
Mcf 7

Natural deep eutectic solvents (NADES) may be considered ‘designer solvents’ due to their numerous structural variations and the possibility of tailoring their physicochemical properties. Prior to their industrial application, characterization of NADES is essential, including determination of their physicochemical properties, cytotoxicity, and antioxidative activity. The most important physicochemical properties of eight prepared NADES (choline chloride:malic acid, proline:malic acid, choline chloride:proline:malic acid, betaine:malic acid, malic acid:glucose, malic acid:glucose:glycerol, choline chloride:citric acid, and betaine:citric acid) were measured as functions of temperature and water content. In general, the structure of prepared NADES greatly influences their physical properties, which could be successfully modified and adjusted by addition of water. All tested NADES were absolutely benign and noncorrosive for investigated steel X6CrNiTi18-10. Furthermore, cytotoxicity of prepared solvents was assessed toward three human cell lines (HEK-293T, HeLa, and MCF-7 cells), and antioxidative activity was measured by the Oxygen Radical Absorbance Capacity (ORAC) method. With regard to cell viability, all tested NADES containing carboxylic acid could be classified as practically harmless and considered environmentally safe. The ORAC values indicated that the tested NADES displayed antioxidative activity.

Identification and characterization of cystatin B from black rockfish, Sebastes schlegelii, indicating its potent immunological importance

2020 ◽  
Vol 104 ◽  
pp. 497-505
Author(s):  
P.D.S.U. Wickramasinghe ◽  
Hyukjae Kwon ◽  
Don Anushka Sandaruwan Elvitigala ◽  
Qiang Wan ◽  
Jehee Lee
Keyword(s):  
Black Rockfish ◽  
Cystatin B ◽  
Sebastes Schlegelii ◽  
Identification And Characterization

scholarly journals Isolation And Characterization Of 38 SNP Markers For The Black Rockfish, Sebastes Schlegelii By Next-Generation Sequencing

2021 ◽  
Author(s):  
Xianggang Gao ◽  
Xiangbo Bao ◽  
Wei Sun ◽  
Yunfeng Li ◽  
Zongying Liu ◽  
...  
Keyword(s):  
Polymorphic Information Content ◽  
Snp Markers ◽  
Habitat Destruction ◽  
Single Nucleotide ◽  
Genetic Studies ◽  
Important Species ◽  
Black Rockfish ◽  
Isolation And Characterization ◽  
Sebastes Schlegelii

Abstract The black rockfish (Sebastes schlegelii) is an economically important species. However, wild S. schlegelii resources have declined sharply in recent years as a result of human disturbance and habitat destruction. Thus, it is crucial to protect the current resources of S. schlegelii. In this study, 38 novel single nucleotide polymorphism (SNP) markers were developed based on restriction-site associated DNA sequencing. The results showed that the observed heterozygosity and expected heterozygosity ranged from 0.1400 to 0.6400 and 0.1487 to 0.4978, respectively. The minor allele frequency raged from 0.1429 to 0.4694. Polymorphic information content ranged from 0.174 to 0.365. Four SNPs were found to be deviated significantly from the HWE (P<0.05). These SNP markers will serve as a useful tool for genetic studies and population evaluation aimed at the conservation of S. schlegelii.

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