scholarly journals Coenzyme Q10, oxidative stress markers, and sperm DNA damage in men with idiopathic oligoasthenoteratospermia

2021 ◽  
Author(s):  
Ahmed T Alahmar ◽  
Pallav Sengupta ◽  
Sulagna Dutta ◽  
Aldo E. Calogero
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Coenzyme Q10 ◽  
Sperm Quality ◽  
Sperm Parameters ◽  
Controlled Study ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna Damage ◽  
Sperm Dna ◽  
Infertile Patients

Objective: Oxidative stress (OS) plays a key role in the etiology of unexplained male infertility. Coenzyme Q10 (CoQ10) is a potent antioxidant that may improve semen quality and OS in infertile men with idiopathic oligoasthenoteratospermia (OAT), but the underlying mechanism is unknown. Therefore, the present study was undertaken to investigate the effect of CoQ10 on OS markers and sperm DNA damage in infertile patients with idiopathic OAT. Methods: This prospective controlled study included 50 patients with idiopathic OAT and 50 fertile men who served as controls. All patients underwent a comprehensive medical assessment. Patients and controls received 200 mg of oral CoQ10 once daily for 3 months. Semen and blood were collected and analyzed for sperm parameters, seminal CoQ10 levels, reactive oxygen species (ROS) levels, total antioxidant capacity, catalase, sperm DNA fragmentation (SDF), and serum hormonal profile. Results: The administration of CoQ10 to patients with idiopathic OAT significantly improved sperm quality and seminal antioxidant status and significantly reduced total ROS and SDF levels compared to pre-treatment values. Conclusion: CoQ10, at a dose of 200 mg/day for 3 months, may be a potential therapy for infertile patients with idiopathic OAT, as it improved sperm parameters and reduced OS and SDF in these patients.

scholarly journals Ejaculate Oxidative Stress Is Related with Sperm DNA Fragmentation and Round Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Valeria Maria Iommiello ◽  
Elena Albani ◽  
Alessandra Di Rosa ◽  
Alessandra Marras ◽  
Francesca Menduni ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Dna Fragmentation ◽  
Sperm Quality ◽  
Reproductive Capacity ◽  
World Health ◽  
Sperm Chromatin ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna Damage ◽  
Sperm Dna

Oxidative stress (OS) plays an essential role in male infertility aetiology by affecting sperm quality, function, and also the integrity of sperm DNA. The assessment of oxidative stress in semen may be an important tool to improve the evaluation of sperm reproductive capacity. The purpose of this study was the evaluation of any possible relation between the unbalance of oxidative stress caused by superoxide anion in the ejaculate with the presence of sperm DNA fragmentation and high concentration of round cells. 56 semen samples from males from couples suffering from infertility were evaluated according to World Health Organisation (WHO) 2010 guidelines. Oxidative stress levels from N1 (low) to N4 (high) were assessed in ejaculates using oxiSperm; DFI (sperm DNA fragmentation index) as assessed by the SCSA (Sperm Chromatin Structure Assay) was used for evaluation of sperm chromatin integrity. Our data show that high oxidative stress (N3-N4 levels) correlated positively with aDFI≥30%(P=0.0379)and round cells≥1.500.000/mL(P=0.0084). In conclusion, OS increases sperm DNA damage. Thus evaluation of semen OS extent of sperm DNA damage in infertile man could be useful to develop new therapeutic strategies and improve success of assisted reproduction techniques (ART).

scholarly journals The aging male: Relationship between male age, sperm quality and sperm DNA damage in an unselected population of 3124 men attending the fertility centre for the first time

2019 ◽  
Vol 90 (4) ◽  
pp. 254-259 ◽  
Author(s):  
Alessandro Colasante ◽  
Maria Giulia Minasi ◽  
Filomena Scarselli ◽  
Valentina Casciani ◽  
Vincenzo Zazzaro ◽  
...  
Keyword(s):  
Dna Damage ◽  
Sperm Morphology ◽  
Semen Quality ◽  
Sperm Quality ◽  
World Health ◽  
Semen Parameters ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna Damage ◽  
Male Age ◽  
Sperm Dna

Objective: the aim of our study was to put forward insights to treat any possible correlation among sperm quality, sperm DNA damage and male age as they may have fertility implications for men who choose to delay fatherhood. Materials and methods: Our study is a non-interventional retrospective analysis of 3124 semen samples from patients that were investigated for the conventional semen parameters. Tunel test assay was set up for the evaluation of the sperm DNA fragmentation index (DFI). We applied the Kappa index to compare both the 1999 and the 2010 World Health Organization (WHO) reference criteria to evaluate the competence of such semen parameters categorization during the standard routine of our laboratory. Results: With regards to our findings, it is possible to underline a significant relationship between aging and semen volume (p = 0.001), motility (p = 0.009), semen viscosity (p < 0.003) and sperm DNA damage (p < 0.009). We found a trend when focusing on the semen concentration (p = 0.05). The analysis of sperm morphology did not show any influence with advancing age (p = 0.606). When comparing both the 1999 and the 2010 WHO scales we found no accordance in the appraisal of sperm morphology but a very good one in the evaluation of the other parameters. Conclusions: Conventional semen analysis represents the opportunity to draw up a proxy insight on the male fertility status even if semen quality can only indirectly assess the probability of pregnancy. Several studies have verified a decay in the male reproductive system, sperm quality and fertility with advancing age although the reported results are not yet conclusive. Our results substantially agree with those findings outlined in the literature. Moreover we find that the discrepancy between the two WHO reference scales would eventually lead to an improper diagnosis of infertility.

scholarly journals The effect of rhFSH on Sperm DNA Fragmentation and sperm parameters in Oligozoospermia Infertile Men

2020 ◽  
Vol 8 (2) ◽  
pp. 55-62
Author(s):  
Atefeh Verdi ◽  
◽  
Seyedeh Saeideh Sahraei ◽  
Elham Asa ◽  
Rahil Jannatifar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Fragmentation ◽  
Follicle Stimulating Hormone ◽  
Sperm Parameters ◽  
Sperm Dna Fragmentation ◽  
Infertile Men ◽  
Sperm Dna ◽  
Before And After ◽  
Infertile Patients ◽  
Stimulating Hormone

Background: One of the main causes of male infertility is the negative effects of oxidative stress. Follicle-stimulating hormone (FSH) plays an essential role in spermatogenesis, as well as in the maintenance of sperm DNA integrity. This study aimed to determine whether the recombinant human follicle-stimulating hormone (rhFSH) treatment of sperm parameters could positively affect sperm DNA and oxidative DNA fragmentation in oligozoospermia infertile men. Materials and Methods: This interventional study was carried out on a sample of 50 oligozoospermia infertile men. To this end, sperm DNA fragmentation and ROS as an oxidative stress marker were measured before and after treatment with the rhFSH sperm parameters. Results: The sperm parameters (concentration, mobility, and morphology) were significantly different in the oligozoospermia infertile patients before and after the rhFSH treatment (p<0.05). Moreover, sperm DNA fragmentation had a significant decrease in the patients after the FSH treatment (p<0.05). In addition, the ROS level in sperm, and the malondialdehyde level of seminal plasma significantly decreased after the treatment (P<0.05). Conclusion: The findings indicated that the rhFSH treatment significantly improved the sperm parameters. Further, the treatment led to a meaningful reduction of sperm DNA fragmentation and oxidative stress in the oligozoospermia infertile patients. Similarly, the malondialdehyde concentration markedly decreased in correlation with DNA fragmentation after the rhFSH treatment.

Evaluation of Sperm DNA Fragmentation via Halosperm Technique and TUNEL Assay Before and After Cryopreservation

2019 ◽  
Vol 26 (12) ◽  
pp. 1575-1581 ◽  
Author(s):  
Senay Cankut ◽  
Turgay Dinc ◽  
Mehmet Cincik ◽  
Guler Ozturk ◽  
Belgin Selam
Keyword(s):  
Dna Damage ◽  
Dna Fragmentation ◽  
Tunel Assay ◽  
University Hospital ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Sperm Dna Fragmentation ◽  
Liquid Form ◽  
Sperm Dna Damage ◽  
Sperm Dna ◽  
Before And After

Aim: Human sperm DNA fragmentation is one of the factors suggested for male infertility. The ratio of sperm DNA damage in semen may adversely affect both the fertilization rate and the embryo development of in vitro fertilization/ intracytoplasmic sperm injection cycles. Sperm cryopreservation both increases the success rates in assisted reproductive techniques (ARTs) and contributes to the preservation of fertility before testis surgery, chemotherapy, and radiotherapy. The aim of the current study is to determine sperm DNA fragmentation, following cryopreservation. Methods: A cross-sectional, observational study was conducted at a university hospital infertility clinic. One hundred (n = 100) volunteer fertile men (ages between 21 and 39 years) with normozoospermic sperm parameters were involved in the current study. Sperm DNA damage was evaluated with the Halosperm technique and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay. Fresh samples were studied in liquid form. The remaining samples were kept frozen and then thawed after 1 month and reevaluated with the Halosperm technique and TUNEL assay. Results were then compared between the fresh and frozen samples. Results: Sperm DNA fragmentation results with the Halosperm technique both before and after cryopreservation were 25% (5%-65%) and 40% (6%-89%), respectively, with a statistically significant increase (15%; P < .001). Sperm DNA fragmentation results by TUNEL assay before and after cryopreservation were 17% (3%-43%) and 36% (7%-94%), respectively, with a statistically significant increase (19%; P <.001). Conclusion: The current data demonstrate increased sperm DNA damage after cryopreservation. Further studies may contribute to development of less harmful techniques and cryoprotectants in order to improve the results of ART.

scholarly journals Sperm DNA Fragmentation: Origin and Impact on Human Reproduction

2011 ◽  
Vol 2 (2) ◽  
pp. 88-108 ◽  
Author(s):  
Ralf R. Henkel ◽  
Daniel R. Franken
Keyword(s):  
Dna Damage ◽  
Environmental Factors ◽  
Dna Fragmentation ◽  
Human Reproduction ◽  
Sperm Dna Fragmentation ◽  
Permanent Damage ◽  
Sperm Dna Damage ◽  
Male Genome ◽  
Sperm Dna ◽  
Early Abortion

Sperm DNA can be damaged due to a multitude of different noxae, which include disease, and occupational and environmental factors. Depending on the magnitude of the damage, such lesions may be repaired by the oocyte or the embryo. If this is not possible, a permanent damage can be manifested leading to mutations of the male genome. In cases where the oocyte or the embryo does not counter these damages to the male genome in terms of repair or an early abortion, sperm DNA damage and fragmentation can be a cause of numerous diseases including childhood cancer.

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