scholarly journals Nitric oxide, 8-hydroxydeoxyguanosine, and total antioxidant capacity in human seminal plasma of infertile men and their relationship with sperm parameters

2020 ◽  
Vol 47 (1) ◽  
pp. 54-60
Author(s):  
Maryam Gholinezhad ◽  
Azadeh Aliarab ◽  
Ghasem Abbaszadeh-Goudarzi ◽  
Yousefreza Yousefnia-Pasha ◽  
Niusha Samadaian ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Male Infertility ◽  
Seminal Plasma ◽  
Total Antioxidant Capacity ◽  
Infertile Men ◽  
Sperm Counts ◽  
Semen Volume ◽  
Total Antioxidant ◽  
The Mean ◽  
Oxidative Biomarkers

Objective: Oxidative stress plays a key role in the pathogenesis of male infertility. But, the adverse effects of oxidative biomarkers on sperm quality remain unclear. This study aimed to investigate the levels of nitric oxide (NO), 8-hydroxydesoxyguanosine (8-OHdG), and total antioxidant capacity (TAC) oxidative biomarkers in seminal plasma and their relationship with sperm parameters.Methods: A total of 77 volunteers participated in the study, including fertile (n=40) and infertile men (n=37). NO, 8-OHdG, and TAC levels were measured using the ferric reducing ability of plasma, Griess reagent method and an enzyme-linked immunosorbent assay kit, respectively.Results: The mean values of sperm parameters in the infertile group were significantly lower than those in the fertile group (<i>p</i><0.001). The mean 8-OHdG in the seminal plasma of infertile men was significantly higher (<i>p</i>=0.013) than those of controls, while the mean TAC was significantly lower (<i>p</i>=0.046). There was no significant difference in NO level between the two groups. The elevated seminal 8-OHdG levels were negatively correlated with semen volume, total sperm counts and morphology (<i>p</i><0.001, <i>p</i>=0.001 and <i>p</i>=0.052, respectively). NO levels were negatively correlated with semen volume, total sperm counts and morphology (<i>p</i>=0.014, <i>p</i>=0.020 and <i>p</i>=0.060, respectively). Positive correlations between TAC and both sperm count and morphology (<i>p</i>=0.043 and <i>p</i>=0.025, respectively) were also found.Conclusion: These results suggested that increased levels of NO and 8-OHdG in seminal plasma could have a negative effect on sperm function by inducing damage to the sperm DNA hence their fertility potentials. Therefore, these biomarkers can be useful in the diagnosis and treatment of male infertility.

scholarly journals Impact of oxidative stress on semen parameters in normozoospermic infertile men: a case–control study

2020 ◽  
Vol 26 (1) ◽  
Author(s):  
Ayad Palani ◽  
Ahmed Alahmar
Keyword(s):  
Oxidative Stress ◽  
Uric Acid ◽  
Male Infertility ◽  
Seminal Plasma ◽  
Case Control Study ◽  
Case Control ◽  
Semen Parameters ◽  
Infertile Men ◽  
Total Antioxidant ◽  
Control Study

Abstract Background Oxidative stress has been implicated in male infertility through decrease in sperm quality. However, men with normal semen parameters (normozoospermia) may be unable to fertilize their partners even when they have normal sperm function. Thus, they would be considered infertile. The purpose of this study was to investigate the role of oxidative stress in the pathogenesis of unexplained male infertility. Methods In this case–control study, infertile men with normozoospermia (n = 46) and fertile control group (n = 21) underwent seminal fluid analyses according to WHO 2010 criteria. Serum and seminal plasma levels of total antioxidant capacity (TAC), glutathione, malondialdehyde, uric acid and albumin were also measured using colorimetric methods. Results The level of total antioxidant capacity in both serum and seminal plasma was significantly lower in normozoospermic infertile men in comparison with fertile group (p < 0.0001). However, no significant differences were observed in serum and seminal plasma levels of glutathione, uric acid, albumin and malondialdehyde between infertile and fertile groups. Conclusion Low TAC level induces oxidative stress and consequently causes sperm dysfunction and male infertility. Estimation of TAC can be a useful tool in the diagnosis of male infertility. Antioxidant supplementation should be considered in the treatment of oxidative stress-induced male infertility.

The AhRR-c.565C>G transversion may increase total antioxidant capacity levels of the seminal plasma in infertile men

2017 ◽  
Vol 24 (21) ◽  
pp. 17428-17435 ◽  
Author(s):  
Gholam Ali Josarayi ◽  
Azadeh Mohammad-Hasani ◽  
Younes Aftabi ◽  
Emadodin Moudi ◽  
Abasalt Hosseinzadeh Colagar
Keyword(s):  
Antioxidant Capacity ◽  
Seminal Plasma ◽  
Total Antioxidant Capacity ◽  
Infertile Men ◽  
Total Antioxidant

scholarly journals Assessment of the Impact of Oxidative Stress on Frozen Seminal Plasma in Fertile and Infertile Men by Examining the Total Antioxidant Capacity

2014 ◽  
Vol 7 (1) ◽  
pp. 25-30
Author(s):  
Violeta S. Rilcheva ◽  
Nina P. Ayvazova ◽  
Danail I. Martinov ◽  
Cvetomir I. Ivanov ◽  
Emiliana I. Konova
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Seminal Plasma ◽  
Total Antioxidant Capacity ◽  
Dna Integrity ◽  
Integrity Test ◽  
Infertile Men ◽  
Total Antioxidant ◽  
The Impact ◽  
Infertile Patients

AbstractThe aim of the study was to explore the impact of oxidative stress on frozen seminal plasma in fertile and infertile men by examining the total antioxidant capacity. Patients: Infertile patients from male infertility clinic with various diagnoses and fertile men. Design: Seminal plasma from proven fertile men [n=50] and infertile patients [n=50] were examined for total antioxidant capacity (TAC) level, semen parameters such as morphology, motility and concentration, and DNA integrity test. Interventions: Seminal plasma TAC measurement by luminometric assay using the TAC assay kit, semen analysis parameters, DNA integrity test. Fertile men showed higher TAC values (median and SD): 1201µM (SD±548), as compared with the infertile patients: 831μM (SD±343). The result from sperm morphology of fertile patients showed a mean percentage of 4.8 % (SD±1.68) whereas the percentage in the infertile group was 2.68% (SD ±1.68). The same group of samples, analyzed for DNA damage showed a mean of DFI 10.38% (SD±5.17%) in fertile men and a mean of DFI 17.22% (SD±7.22%) in infertile men. Total antioxidant capacity of the seminal plasma as measured by the luminоmetric assay is a reliable and simple test for diagnosing and management of male infertility.

Total antioxidant capacity of seminal plasma is different in fertile and infertile men

1995 ◽  
Vol 64 (4) ◽  
pp. 868-870 ◽  
Author(s):  
Sheena E.M. Lewis ◽  
Paul M. Boyle ◽  
Karen A. McKinney ◽  
Ian S. Young ◽  
William Thompson
Keyword(s):  
Antioxidant Capacity ◽  
Seminal Plasma ◽  
Total Antioxidant Capacity ◽  
Infertile Men ◽  
Total Antioxidant

Use of total antioxidant capacity as a diagnostic test in seminal plasma of infertile men

2015 ◽  
Vol 104 (3) ◽  
pp. e147
Author(s):  
G. Ahmad ◽  
S. Gupta ◽  
R. Sharma ◽  
E. Sabanegh ◽  
A. Agarwal
Keyword(s):  
Antioxidant Capacity ◽  
Diagnostic Test ◽  
Seminal Plasma ◽  
Total Antioxidant Capacity ◽  
Infertile Men ◽  
Total Antioxidant

Evaluation of Salivary Antioxidants and Oxidative Stress Markers in Male Smokers

2019 ◽  
Vol 22 (7) ◽  
pp. 496-501
Author(s):  
Fatemeh Ahmadi-Motamayel ◽  
Parisa Falsafi ◽  
Hamidreza Abolsamadi ◽  
Mohammad T. Goodarzi ◽  
Jalal Poorolajal
Keyword(s):  
Oxidative Stress ◽  
Uric Acid ◽  
Free Radicals ◽  
Antioxidant Capacity ◽  
Cigarette Smoke ◽  
Total Antioxidant Capacity ◽  
The Body ◽  
Total Antioxidant ◽  
The Mean ◽  
And Oxidative Stress

Background: Cigarette smoke free radicals can cause cellular damage and different diseases. All the body fluids have antioxidants which protect against free radicals. Objective: The aim of this study was to evaluate salivary total antioxidant capacity and peroxidase, uric acid and malondialdehyde levels in smokers and a nonsmoking control group. Methods: Unstimulated saliva was collected from 510 males. A total of 259 subjects were current smokers and 251 were non-smokers. The levels of salivary total antioxidant capacity, uric acid, peroxidase and malondialdehyde were measured using standard procedures. Data were analyzed with t test and ANOVA. Results: The smokers were younger and dental hygiene index was higher than healthy nonsmoking controls. The mean total antioxidant capacity in smokers and nonsmokers was 0.13±0.07 and 0.21±011, respectively (P=0.001). Smokers had significantly lower peroxidase and uric acid levels than healthy controls. In addition, the mean malondialdehyde levels in the smokers and nonsmokers were 4.55 ±2.61 and 2.79 ±2.21, respectively (P=0.001). Conclusion: Cigarette smoke produces free radical and oxidative stress, causing many side effects. Salivary antioxidant levels decreased and malondialdehyde levels increased in smokers, indicating the high oxidative stress among smokers compared to nonsmokers. Cigarette smoke had deleterious effects on main salivary antioxidants levels.

scholarly journals Role of Glutathione in Male Infertility

2013 ◽  
Vol 4 (2) ◽  
pp. 20-25
Author(s):  
ZU Naher ◽  
SK Biswas ◽  
FH Mollah ◽  
M Ali ◽  
MI Arslan
Keyword(s):  
Male Infertility ◽  
Seminal Plasma ◽  
Antioxidant Defense ◽  
Sperm Morphology ◽  
Sperm Count ◽  
Antioxidant Systems ◽  
Infertile Male ◽  
Semen Volume ◽  
Infertile Group ◽  
Male Subjects

Infertility is a worldwide problem and in almost 50% of cases infertility results from abnormality of the male partners. Apart from endocrine disorders, definitive cause and mechanism of male infertility is not clear in many cases. Recent evidence indicates that imbalance between pro-oxidant stress and antioxidant defense plays an important role in the pathogenesis of male infertility. Among the endogenous antioxidant systems, reduced glutathione (GSH) plays a significant role in the antioxidant defense of the spermatogenic epithelium, the epididymis and perhaps in the ejaculated spermatozoa. The current study was therefore designed to evaluate any association that may exist between GSH levels and male infertility. Infertile male patients (having female partners with normal fertility parameters; n=31) and age- matched healthy male fertile control subjects (n=30) were included in this study. In addition to medical history, semen analyses including semen volume, sperm count, motility and morphology were done for each subject. As a measure of antioxidant capacity erythrocyte and seminal plasma GSH concentrations were measured by Ellman's method in fertile and infertile male subjects. The infertile subjects were similar to fertile subjects in terms of age. However, semen volume and sperm count was found significantly lower (p<0.001) in infertile males compared with healthy fertile male subjects. Percentage of subjects with abnormal sperm morphology and motility were found higher in infertile group compared with fertile group. The median (range) erythrocyte GSH level did not differ between the two groups (12.62 (0.67-29.82) versus 13.93 (2.10-21.08) mg/gm Hb). However, the seminal plasma GSH level was found markedly suppressed in infertile group (1.64 (0.23-7.50)) compared with fertile group (4.26 (2.32-7.50)) mg/dl (p<0.001). In the present study seminal plasma GSH level was found markedly suppressed along with abnormal values for semen volume, sperm concentration and sperm morphology and motility in infertile subjects compared with fertile subjects. This finding indicates that low level of seminal plasma GSH level may be associated with male infertility. DOI: http://dx.doi.org/10.3329/bjmb.v4i2.13772 Bangladesh J Med Biochem 2011; 4(2): 20-25

scholarly journals High total antioxidant capacity of the porcine seminal plasma (SP-TAC) relates to sperm survival and fertility

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Isabel Barranco ◽  
Asta Tvarijonaviciute ◽  
Cristina Perez-Patiño ◽  
Inmaculada Parrilla ◽  
Jose J. Ceron ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Seminal Plasma ◽  
Total Antioxidant Capacity ◽  
Total Antioxidant ◽  
Sperm Survival
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