scholarly journals Polar Lipid Fraction from Golden Oyster Mushrooms (Pleurotus citrinopileatus) Suppresses Colon Injuries from Inflammatory Stresses in vivo and in vitro

2020 ◽  
Vol 69 (7) ◽  
pp. 751-757
Author(s):  
Shinji Yamashita ◽  
Takuya Seino ◽  
Miho Inobe ◽  
Mirinthorn Jutanom ◽  
Satoshi Matsumoto ◽  
...  
Keyword(s):  
Polar Lipid ◽  
Lipid Fraction ◽  
Polar Lipid Fraction ◽  
Oyster Mushrooms ◽  
Pleurotus Citrinopileatus ◽  
Colon Injuries

Analysis of polar lipid fraction of Pinus halepensis Mill. seeds from North Algeria

2013 ◽  
Vol 51 ◽  
pp. 116-122 ◽  
Author(s):  
Nabil Kadri ◽  
Bachra Khettal ◽  
Rachida Yahiaoui-zaidi ◽  
Veronique Barragan-Montero ◽  
Jean-Louis Montero
Keyword(s):  
Polar Lipid ◽  
Pinus Halepensis ◽  
Lipid Fraction ◽  
Polar Lipid Fraction ◽  
North Algeria

Neutral lipids, phospholipids, and a betaine lipid of the snow mold fungus Microdochium nivale

1998 ◽  
Vol 44 (11) ◽  
pp. 1051-1059 ◽  
Author(s):  
Anita Istokovics ◽  
Naoki Morita ◽  
Kazuo Izumi ◽  
Tamotsu Hoshino ◽  
Isao Yumoto ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Neutral Lipid ◽  
Polar Lipid ◽  
Lipid Fraction ◽  
Microdochium Nivale ◽  
Neutral Lipid Fraction ◽  
Polar Lipid Fraction ◽  
Snow Mold ◽  
Betaine Lipid

The hyphae of the snow mold Microdochium nivale contained lipids in a yield of about 10% w/w of the dry matter of hyphae. The total lipid was fractionated into neutral and polar lipid fractions. In the neutral lipid fraction, triacylglylcerol was the sole major component. As minor components, ergosterol, diacylglycerol, free fatty acid, and fatty acyl ergosterol were identified. The polar lipid fraction contained phospholipids, glycolipids, and a lipid containing neither phosphorus nor sugar. Phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, phosphatidylserine, and phosphatidic acid were identified as phospholipids. The polar lipid fraction included at least four kinds of glycolipids that have not been identified. A very unusual lipid in fungi, a betaine lipid, diacylglyceryltrimethylhomoserine, was identified by chemical and physicochemical analyses. The level of the neutral lipid fraction, which accounted for 60% of the total lipid in hyphae at the exponential phase, was significantly increased compared with that of the polar lipid fraction and constituted 80% of the total at the stationary phase. The neutral and polar lipids of Microdochium nivale contained 18:3 (9,12,15), 18:2 (9,12), 18:1 (9), and 16:0 as principal fatty acids. Among them, 18:2 (9,12) and 18:3 (9,12,15) were the major fatty acids of triacylglycerol, phosphatidylcholine, phosphatidylglycerol, and phosphatidylethanolamine, whereas in diacylglyceryltrimethylhomoserine, the major components were 16:0 and 18:3 (9,12,15).Key words: snow mold, phospholipids, betaine lipid, fatty acid, Microdochium nivale.

scholarly journals Polar Lipid Fraction E from Sulfolobus acidocaldarius and Dipalmitoylphosphatidylcholine Can Form Stable yet Thermo-Sensitive Tetraether/Diester Hybrid Archaeosomes with Controlled Release Capability

2020 ◽  
Vol 21 (21) ◽  
pp. 8388
Author(s):  
Umme Ayesa ◽  
Parkson Lee-Gau Chong
Keyword(s):  
Drug Release ◽  
Zeta Potential ◽  
Polar Lipid ◽  
Sharp Increase ◽  
Serum Proteins ◽  
Release Kinetics ◽  
Lipid Fraction ◽  
Sulfolobus Acidocaldarius ◽  
Polar Lipid Fraction ◽  
Flip Flop

Archaeosomes have drawn increasing attention in recent years as novel nano-carriers for therapeutics. The main obstacle of using archaeosomes for therapeutics delivery has been the lack of an efficient method to trigger the release of entrapped content from the otherwise extremely stable structure. Our present study tackles this long-standing problem. We made hybrid archaeosomes composed of tetraether lipids, called the polar lipid fraction E (PLFE) isolated from the thermoacidophilic archaeon Sulfolobus acidocaldarius, and the synthetic diester lipid dipalmitoylphosphatidylcholine (DPPC). Differential polarized phase-modulation and steady-state fluorometry, confocal fluorescence microscopy, zeta potential (ZP) measurements, and biochemical assays were employed to characterize the physical properties and drug behaviors in PLFE/DPPC hybrid archaeosomes in the presence and absence of live cells. We found that PLFE lipids have an ordering effect on fluid DPPC liposomal membranes, which can slow down the release of entrapped drugs, while PLFE provides high negative charges on the outer surface of liposomes, which can increase vesicle stability against coalescence among liposomes or with cells. Furthermore, we found that the zeta potential in hybrid archaeosomes with 30 mol% PLFE and 70 mol% DPPC (designated as PLFE/DPPC(3:7) archaeosomes) undergoes an abrupt increase from −48 mV at 37 °C to −16 mV at 44 °C (termed the ZP transition), which we hypothesize results from DPPC domain melting and PLFE lipid ‘flip-flop’. The anticancer drug doxorubicin (DXO) can be readily incorporated into PLFE/DPPC(3:7) archaeosomes. The rate constant of DXO release from PLFE/DPPC(3:7) archaeosomes into Tris buffer exhibited a sharp increase (~2.5 times), when the temperature was raised from 37 to 42 °C, which is believed to result from the liposomal structural changes associated with the ZP transition. This thermo-induced sharp increase in drug release was not affected by serum proteins as a similar temperature dependence of drug release kinetics was observed in human blood serum. A 15-min pre-incubation of PLFE/DPPC(3:7) archaeosomal DXO with MCF-7 breast cancer cells at 42 °C caused a significant increase in the amount of DXO entering into the nuclei and a considerable increase in the cell’s cytotoxicity under the 37 °C growth temperature. Taken together, our data suggests that PLFE/DPPC(3:7) archaeosomes are stable yet potentially useful thermo-sensitive liposomes wherein the temperature range (from 37 to 42–44 °C) clinically used for mild hyperthermia treatment of tumors can be used to trigger drug release for medical interventions.

Polar lipid fraction in soil: a kerogen-like matter

1991 ◽  
Vol 17 (3) ◽  
pp. 341-349 ◽  
Author(s):  
A. Amblès ◽  
J.C. Jacquesy ◽  
P. Jambu ◽  
J. Joffre ◽  
R. Maggi-Churin
Keyword(s):  
Polar Lipid ◽  
Lipid Fraction ◽  
Polar Lipid Fraction

scholarly journals Seasonal Variations of Lipid Content and Composition in Starfish Asterias amurensis Lütken

2012 ◽  
Vol 15 (1) ◽  
pp. 45 ◽  
Author(s):  
A.K.M. Azad Shah ◽  
H. Kurihara ◽  
K. Takahashi
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Lipid Content ◽  
Polar Lipid ◽  
Lipid Fraction ◽  
Total Lipid Content ◽  
Polar Lipid Fraction ◽  
Internal Organs ◽  
Asterias Amurensis ◽  
Lipid Fractions

The total lipid content, lipid classes and fatty acid composition in the internal organs of starfish (Asterias amurensis) were analyzed to determine the effects of seasons (winter and spring). The non-polar and polar lipid fractions obtained from starfish internal organs were analyzed through two seasons using thin layer chromatography (TLC) and gas liquid chromatography. Total lipid content of internal organs was 10.18% in spring and 8.21% in winter as wet weight basis. The predominant lipid class in spring was triglyceride whereas free fatty acids were the main lipid class in winter. The most abundant fatty acid of non-polar lipid fraction was eicosamonoenoic acid (C20:1) in spring having the highest proportion (29.2% of total fatty acid) as compared to the winter. Eicosapentaenoic acid (EPA) was also found significantly (P < 0.05) higher in spring compared to winter in the non-polar lipid fraction. On the other hand, comparatively lower amount of EPA was observed in spring than winter in the polar lipid fraction. Proportions of other fatty acids in non-polar and polar lipid fractions were also varied seasonally. This result might be useful for commercial production of lipid from internal organ of starfish with a view to potential use in food, pharmaceutical, cosmetics and other non-food industries.

Polar lipid fraction from oat (Avena sativa): characterization and use as an o/w emulsifier

2012 ◽  
Vol 235 (3) ◽  
pp. 507-515 ◽  
Author(s):  
Mika Kaimainen ◽  
Suvi Ahvenainen ◽  
Mirka Kaariste ◽  
Eila Järvenpää ◽  
Martti Kaasalainen ◽  
...  
Keyword(s):  
Polar Lipid ◽  
Avena Sativa ◽  
Lipid Fraction ◽  
Polar Lipid Fraction

Separation and Purification of Wax from Nyamplung (Calophyllum inophyllum) Seed Oil

2019 ◽  
Vol 964 ◽  
pp. 1-6
Author(s):  
Safrina Hapsari ◽  
David Febrilliant Susanto ◽  
Hakun Wirawasista Aparamarta ◽  
Arief Widjaja ◽  
Setiyo Gunawan
Keyword(s):  
Solvent Extraction ◽  
Crude Oil ◽  
Polar Lipid ◽  
Seed Oil ◽  
Lipid Fraction ◽  
Raw Material ◽  
Water Repellent ◽  
Polar Lipid Fraction ◽  
Calophyllum Inophyllum ◽  
Solvent Crystallization

Nyamplung (Calophyllum inophyllum) is a multi-functional plant which is spread widely over the coast of Indonesia. Its seed produces a high content of oil, but its utilization is still limited. It is because C. inophyllum seed oil contains toxic compounds. Therefore, C. inophyllum seed oil has been used as a biodiesel raw material for many years. It was reported that C. inophyllum seed oil contains wax, but its percentage remains unknown. Wax has been used in cosmetics, pharmaceuticals, foods, and coatings industries as oil binder, water repellent, scratch resistance, and dispersion medium. In this work, wax was separated from C. inophyllum seed oil by solvent crystallization with and without separating non-polar lipid fraction (NPLF) from crude oil. Non-polar lipid fraction was separated by batch-wise solvent extraction using petroleum ether to methanol mass ratio of 3:1 (w/w) for eight stages. After eight stages, non-polar lipid fraction was collected for further separation by solvent crystallization method. The ratios of non-polar lipid fraction to acetone were 1:10, 1:20, and 1:40 (w/v). Then, the isolated wax was analyzed by gas chromatography. It was found that wax (purity of 40% and yield of 0.35%) was successfully isolated by separating non-polar lipid fraction from crude oil (batch wise solvent extraction for eight stages) and followed by solvent crystallization (non-polar lipid fraction to acetone ratio of 1:40 (w/v)).

scholarly journals Influence of carbohydrate- and protein-based foods on the formation of polar lipid fraction during deep-frying

Food Control ◽  
2020 ◽  
Vol 107 ◽  
pp. 106781 ◽  
Author(s):  
Yih Phing Khor ◽  
Biow Ing Sim ◽  
Faridah Abas ◽  
Oi Ming Lai ◽  
Yong Wang ◽  
...  
Keyword(s):  
Polar Lipid ◽  
Lipid Fraction ◽  
Deep Frying ◽  
Polar Lipid Fraction
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