scholarly journals Effect of Membrane-bound Aldehyde Dehydrogenase-encoding Gene Disruption on Glyceric Acid Production in Gluconobacter oxydans

2014 ◽  
Vol 63 (9) ◽  
pp. 953-957 ◽  
Author(s):  
Hiroshi Habe ◽  
Shun Sato ◽  
Tokuma Fukuoka ◽  
Dai Kitamoto ◽  
Keiji Sakaki
Keyword(s):  
Aldehyde Dehydrogenase ◽  
Acid Production ◽  
Gene Disruption ◽  
Gluconobacter Oxydans ◽  
Glyceric Acid ◽  
Membrane Bound ◽  
Encoding Gene

scholarly journals Membrane-Bound Alcohol Dehydrogenase Is Essential for Glyceric Acid Production in Acetobacter tropicalis

2011 ◽  
Vol 60 (9) ◽  
pp. 489-494 ◽  
Author(s):  
Hiroshi Habe ◽  
Shun Sato ◽  
Tokuma Fukuoka ◽  
Dai Kitamoto ◽  
Toshiharu Yakushi ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Acid Production ◽  
Glyceric Acid ◽  
Membrane Bound

scholarly journals Membrane-Bound Pyrroloquinoline Quinone-Dependent Dehydrogenase in Gluconobacter oxydans M5, Responsible for Production of 6-(2-Hydroxyethyl) Amino-6-Deoxy-l-Sorbose

2008 ◽  
Vol 74 (16) ◽  
pp. 5250-5253 ◽  
Author(s):  
Xue-Peng Yang ◽  
Liu-Jing Wei ◽  
Jin-Ping Lin ◽  
Bo Yin ◽  
Dong-Zhi Wei
Keyword(s):  
Gene Disruption ◽  
Gluconobacter Oxydans ◽  
Pyrroloquinoline Quinone ◽  
Sorbitol Dehydrogenase ◽  
Antidiabetic Drug ◽  
Dehydrogenase Gene ◽  
Membrane Bound

ABSTRACT A membrane-bound protein purified from Gluconobacter oxydans M5 was confirmed to be a pyrroloquinoline quinone-dependent d-sorbitol dehydrogenase. Gene disruption and complementation experiments demonstrated that this enzyme is responsible for the oxidation of 1-(2-hydroxyethyl) amino-1-deoxy-d-sorbitol (1NSL) to 6-(2-hydroxyethyl) amino-6-deoxy-l-sorbose (6NSE), which is the precursor of an antidiabetic drug, miglitol.

scholarly journals Gluconobacter oxydans Knockout Collection Finds Improved Rare Earth Element Extraction

2021 ◽  
Author(s):  
Alexa M. Schmitz ◽  
Brooke Pian ◽  
Sean Medin ◽  
Matthew C. Reid ◽  
Mingming Wu ◽  
...  
Keyword(s):  
Rare Earth ◽  
Acid Production ◽  
Glucose Dehydrogenase ◽  
Gluconobacter Oxydans ◽  
Extraction Methods ◽  
Promising Alternative ◽  
Energy Technologies ◽  
Thermochemical Processes ◽  
Membrane Bound ◽  
Critical Components

Rare earth elements (REE) are critical components of our technological society and essential for renewable energy technologies. Traditional thermochemical processes to extract REE from mineral ores or recycled materials are costly and environmentally harmful, and thus more sustainable extraction methods require exploration. Bioleaching offers a promising alternative to conventional REE extraction, and is already used to extract 5% of the world's gold, and approximately 15% of the world's copper supply. However, the performance of REE bioleaching lags far behind thermochemical processes. Despite this, to the best of our knowledge no genetic engineering strategies have yet been used to enhance REE bioleaching, and little is known of the genetics that confer this capability. Here we build a whole genome knockout collection for Gluconobacter oxydans B58, one of the most promising organisms for REE bioleaching, and use it to comprehensively characterize the genomics of REE bioleaching. In total, we find 304 genes that notably alter production of G. oxydans' acidic biolixiviant, including 165 that hold up under statistical comparison with wild-type. The two most impactful groups of genes involved in REE bioleaching have opposing influences on acid production and REE bioleaching. Disruption of genes underlying synthesis of the cofactor pyrroloquinoline quinone (PQQ) and the PQQ-dependent membrane-bound glucose dehydrogenase all but eliminates bioleaching. In contrast, disruption of the phosphate-specific transport system accelerates acid production and enhances bioleaching. We identified 6 disruption mutants, that increase bioleaching by at least 11%. Most significantly, disruption of pstC, encoding part of the phosphate -specific transporter, pstSCAB, enhances bioleaching by 18%. Taken together, these results give a comprehensive roadmap for engineering multiple sites in the genome of G. oxydans to further increase its bioleaching efficiency.

scholarly journals Microbial Production of Glyceric Acid, an Organic Acid That Can Be Mass Produced from Glycerol

2009 ◽  
Vol 75 (24) ◽  
pp. 7760-7766 ◽  
Author(s):  
Hiroshi Habe ◽  
Yuko Shimada ◽  
Toshiharu Yakushi ◽  
Hiromi Hattori ◽  
Yoshitaka Ano ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Growth Parameters ◽  
Operating Time ◽  
Enantiomeric Excess ◽  
Gluconobacter Oxydans ◽  
Acetic Acid Bacteria ◽  
Culture Broth ◽  
Glyceric Acid ◽  
Bacterial Strains ◽  
Biotechnological Production

ABSTRACT Glyceric acid (GA), an unfamiliar biotechnological product, is currently produced as a small by-product of dihydroxyacetone production from glycerol by Gluconobacter oxydans. We developed a method for the efficient biotechnological production of GA as a target compound for new surplus glycerol applications in the biodiesel and oleochemical industries. We investigated the ability of 162 acetic acid bacterial strains to produce GA from glycerol and found that the patterns of productivity and enantiomeric GA compositions obtained from several strains differed significantly. The growth parameters of two different strain types, Gluconobacter frateurii NBRC103465 and Acetobacter tropicalis NBRC16470, were optimized using a jar fermentor. G. frateurii accumulated 136.5 g/liter of GA with a 72% d-GA enantiomeric excess (ee) in the culture broth, whereas A. tropicalis produced 101.8 g/liter of d-GA with a 99% ee. The 136.5 g/liter of glycerate in the culture broth was concentrated to 236.5 g/liter by desalting electrodialysis during the 140-min operating time, and then, from 50 ml of the concentrated solution, 9.35 g of GA calcium salt was obtained by crystallization. Gene disruption analysis using G. oxydans IFO12528 revealed that the membrane-bound alcohol dehydrogenase (mADH)-encoding gene (adhA) is required for GA production, and purified mADH from G. oxydans IFO12528 catalyzed the oxidation of glycerol. These results strongly suggest that mADH is involved in GA production by acetic acid bacteria. We propose that GA is potentially mass producible from glycerol feedstock by a biotechnological process.

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