scholarly journals Synthesis and Evaluation of Dioleoyl Glyceric Acids Showing Antitrypsin Activity

2011 ◽  
Vol 60 (6) ◽  
pp. 327-331 ◽  
Author(s):  
Hiroshi Habe ◽  
Tokuma Fukuoka ◽  
Shun Sato ◽  
Dai Kitamoto ◽  
Keiji Sakaki
Keyword(s):  
1981 ◽  
Vol 22 (3-4) ◽  
pp. 360-368
Author(s):  
T. Honkanen-Buzalski ◽  
T. Katila ◽  
M. Sandholm

1982 ◽  
Vol 28 (4) ◽  
pp. 615-617 ◽  
Author(s):  
A P Orfanos ◽  
E W Naylor ◽  
R Guthrie

Abstract We describe a fluorescent spot test for detecting alpha 1-antitrypsin activity in dried-blood specimens. The eluate of a blood disc is mixed with carbobenzoxy-L-arginine-7-amino-4-methylcoumarin amide and trypsin at the appropriate pH. In the absence of alpha 1-antitrypsin, aminomethylcoumarin, a strongly fluorescent compound, is released. The reaction mixture, when spotted on chromatography paper and viewed under ultraviolet light, exhibits a bright fluorescence only in the case of specimens with alpha 1-antitrypsin deficiency. alpha 1-Antitrypsin activity so estimated correlated well with quantitative assays of dried-blood spots and serum. The procedure is simple and inexpensive, and has the potential for use as a screening test.


1984 ◽  
Vol 51 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Markus Sandholm ◽  
Tuula Honkanen-Buzalski ◽  
Reima Kangasniemi

SummaryAs mastitis is associated with leakage of small molecular weight plasma proteins, such as α1-antitrypsin, into milk, this antitrypsin can be used as an indicator of mastitis. A colorimetric procedure was developed for large scale monitoring of milk antitrypsin activity, using microtitration plates and the Multiskan system. The effect of stage of lactation and age of the cow on the antitrypsin concentration and its interrelationship with other mastitis indicators (bovine serum albumin (BSA), somatic cell count) was analysed by computer programs on 1029 cows. Milk antitrypsin activity was high after parturition owing to colostral inhibitors. After the first month of lactation the assay measures only blood-derived antitrypsin and is a good indicator for detecting an increased permeability between blood and milk due to mastitis. Increasing lactation number only slightly affected the antitrypsin and BSA concentrations whereas somatic cell content was markedly affected.


1982 ◽  
Vol 28 (4) ◽  
pp. 446-451 ◽  
Author(s):  
Parimal Chowdhury ◽  
Donald B. Louria ◽  
Louis W. Chang ◽  
Phillip L. Rayford

1973 ◽  
Vol 26 (1) ◽  
pp. 48-51 ◽  
Author(s):  
P. T. Rowley ◽  
D. Oette

1993 ◽  
Vol 374 (7-12) ◽  
pp. 851-854 ◽  
Author(s):  
Jan RÓŻYCKI ◽  
Gotfryd KUPRYSZEWSKI ◽  
Krzysztof ROLKA ◽  
Ulf RAGNARSSON ◽  
Tomasz ZBYTRYT ◽  
...  
Keyword(s):  

2006 ◽  
Vol 43 (4) ◽  
pp. 88-92 ◽  
Author(s):  
B. Lisowska-Myjak ◽  
J. Pachecka ◽  
B. Kaczyńska ◽  
G. Miszkurka ◽  
K. Kądziela

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