scholarly journals Limonene Suppresses Lipopolysaccharide-Induced Production of Nitric Oxide, Prostaglandin E2, and Pro-inflammatory Cytokines in RAW 264.7 Macrophages

2010 ◽  
Vol 59 (8) ◽  
pp. 415-421 ◽  
Author(s):  
Weon-Jong Yoon ◽  
Nam Ho Lee ◽  
Chang-Gu Hyun
Keyword(s):  
Nitric Oxide ◽  
Prostaglandin E2 ◽  
Inflammatory Cytokines ◽  
Raw 264.7 ◽  
Raw 264.7 Macrophages ◽  
Pro Inflammatory Cytokines

Inhibitory effects of ivermectin on nitric oxide and prostaglandin E2 production in LPS-stimulated RAW 264.7 macrophages

2009 ◽  
Vol 9 (3) ◽  
pp. 354-359 ◽  
Author(s):  
Xuemei Zhang ◽  
Yu Song ◽  
Huanzhang Xiong ◽  
Xinxin Ci ◽  
Hongyu Li ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Prostaglandin E2 ◽  
Raw 264.7 ◽  
Inhibitory Effects ◽  
Raw 264.7 Macrophages

scholarly journals Polyamine Transport Protein PotD Protects Mice against Haemophilus parasuis and Elevates the Secretion of Pro-Inflammatory Cytokines of Macrophage via JNK–MAPK and NF–κB Signal Pathways through TLR4

Vaccines ◽  
2019 ◽  
Vol 7 (4) ◽  
pp. 216 ◽  
Author(s):  
Ke Dai ◽  
Xiaoyu Ma ◽  
Zhen Yang ◽  
Yung-Fu Chang ◽  
Sanjie Cao ◽  
...  
Keyword(s):  
Immune Responses ◽  
Inflammatory Cytokines ◽  
Transport System ◽  
Signal Pathways ◽  
Raw 264.7 ◽  
Raw 264.7 Macrophages ◽  
Cellular Immune Responses ◽  
Polyamine Transport ◽  
Cellular Immune ◽  
Pro Inflammatory Cytokines

The potD gene, belonging to the well-conserved ABC (ATP-binding cassette) transport system potABCD, encodes the bacterial substrate-binding subunit of the polyamine transport system. In this study, we found PotD in Haemophilus (Glaesserella) parasuis could actively stimulate both humoral immune and cellular immune responses and elevate lymphocyte proliferation, thus eliciting a Th1-type immune response in a murine immunity and infection model. Stimulation of Raw 264.7 macrophages with PotD validated that Toll-like receptor 4, rather than 2, participated in the positive transcription and expression of pro-inflammatory cytokines IL–1β, IL–6, and TNF–α using qPCR and ELISA. Blocking signal-regulated JNK–MAPK and RelA(p65) pathways significantly decreased PotD-induced pro-inflammatory cytokine production. Overall, we conclude that vaccination of PotD could induce both humoral and cellular immune responses and provide immunoprotection against H. parasuis challenge. The data also suggest that Glaesserella PotD is a novel pro-inflammatory mediator and induces TLR4-dependent pro-inflammatory activity in Raw 264.7 macrophages through JNK–MAPK and RelA(p65) pathways.

scholarly journals Enhanced immune response by vacuoles isolated from Saccharomyces cerevisiae in RAW 264.7 macrophages

2021 ◽  
Vol 41 (9) ◽  
Author(s):  
Su-Min Lee ◽  
Wooil Choi ◽  
Woo-Ri Shin ◽  
Yang-Hoon Kim ◽  
Jiho Min
Keyword(s):  
Nitric Oxide ◽  
Saccharomyces Cerevisiae ◽  
Immune Response ◽  
Inflammatory Cytokines ◽  
Membrane Vesicles ◽  
Raw 264.7 Cells ◽  
No Production ◽  
Raw 264.7 ◽  
Tnf Α ◽  
Pro Inflammatory Cytokines

Abstract Vacuoles are membrane vesicles in eukaryotic cells, the digestive system of cells that break down substances absorbed outside the cell and digest the useless components of the cell itself. Researches on anticancer and intractable diseases using vacuoles are being actively conducted. The practical application of the present study to animals requires the determination of the biocompatibility of vacuole. In the present study, we evaluated the effects of vacuoles isolated from Saccharomyces cerevisiae in RAW 264.7 cells. This showed a significant increase in the production of nitric oxide (NO) produced by macrophage activity. Using Reactive Oxygen Species (ROS) assay, we identified that ROS is increased in a manner dependent on vacuole concentration. Western blot analysis showed that vacuole concentration-dependently increased protein levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2). Therefore, iNOS expression was stimulated to induce NO production. In addition, pro-inflammatory cytokines levels promoted, such as interleukin (IL) 6 (IL-6) and tumor necrosis factor (TNF) α (TNF-α). In summary, vacuoles activate the immune response of macrophages by promoting the production of immune-mediated transporters NO, ROS, and pro-inflammatory cytokines.

scholarly journals Curcumin abrogates LPS-induced pro-inflammatory cytokines in RAW 264.7 macrophages. Evidence for novel mechanisms involving SOCS-1, -3 and p38 MAPK

2013 ◽  
Vol 58 (10) ◽  
pp. 1309-1317 ◽  
Author(s):  
Morgana Rodrigues Guimarães ◽  
Fábio Renato Manzoli Leite ◽  
Luís Carlos Spolidorio ◽  
Keith Lough Kirkwood ◽  
Carlos Rossa
Keyword(s):  
P38 Mapk ◽  
Inflammatory Cytokines ◽  
Raw 264.7 ◽  
Raw 264.7 Macrophages ◽  
Pro Inflammatory Cytokines
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