scholarly journals Kinetic Resolution of Enantiomers in Racemic and Enantiomerically Enriched 2-Alkanols by Pseudomonas cepacia Lipase Catalyzed Transesterification with Isopropenyl Acetate in Organic Solvent

2005 ◽  
Vol 54 (1) ◽  
pp. 21-31 ◽  
Author(s):  
Yoshimi KANAMORI ◽  
Michitoshi WATANABE ◽  
Keiyo KAWAUCHI ◽  
Yun-Gang CHEN ◽  
Hiroshi YANAGISHITA ◽  
...  
Keyword(s):  
Organic Solvent ◽  
Kinetic Resolution ◽  
Pseudomonas Cepacia ◽  
Resolution Of Enantiomers ◽  
Isopropenyl Acetate

scholarly journals Kinetic Resolution of (R)- and (S)-3-Alkanols by Lipase Catalyzed Transesterification with Isopropenyl Acetate in Organic Solvent

2005 ◽  
Vol 54 (2) ◽  
pp. 105-114
Author(s):  
Yoshimi KANAMORI ◽  
Michitoshi WATANABE ◽  
Yun-Gang CHEN ◽  
Hiroshi YANAGISHITA ◽  
Hirofumi HIRATA
Keyword(s):  
Organic Solvent ◽  
Kinetic Resolution ◽  
Isopropenyl Acetate

scholarly journals Kinetic Resolution of Optically Pure 3-Alkanol by Pseudomonas cepacia Lipase-Catalyzed Transesterification with Vinyl Acetate in Organic Solvent

2003 ◽  
Vol 52 (7) ◽  
pp. 375-386 ◽  
Author(s):  
Hirofumi HIRATA ◽  
Masako IKUSHIMA ◽  
Michitoshi WATANABE ◽  
Keiyo KAWAUCHI ◽  
Masato MIYAGISHI ◽  
...  
Keyword(s):  
Organic Solvent ◽  
Vinyl Acetate ◽  
Kinetic Resolution ◽  
Pseudomonas Cepacia ◽  
Pseudomonas Cepacia Lipase ◽  
Optically Pure

Amano Lipase PS from Burkholderia cepacia- Evaluation of the Effect of Substrates and Reaction Media on the Catalytic Activity

2020 ◽  
Vol 24 (7) ◽  
pp. 798-807
Author(s):  
Jacek Dulęba ◽  
Tomasz Siódmiak ◽  
Michał Piotr Marszałł
Keyword(s):  
Burkholderia Cepacia ◽  
Kinetic Resolution ◽  
Lipolytic Activity ◽  
Reaction Medium ◽  
Building Blocks ◽  
Diisopropyl Ether ◽  
Acyl Donor ◽  
Pseudomonas Cepacia ◽  
Reaction Media ◽  
Isopropenyl Acetate

: Lipases in the native or immobilized form have commonly been used as catalysts in the chemical and pharmaceutical industry. One of the widely available enzyme catalysts on the market is lipase from Burkholderia cepacia (BCLs), previously called Pseudomonas cepacia (PCLs). This enzyme is applied, among others, in the stereoselective acylation of molecules to achieve chiral pure enantiomers of drugs or their building blocks. In this study, Amano lipase PS (APS-BCL), which is a commercial lipase from Burkholderia cepacia (BC) was tested. The lipolytic activity of APS-BCL by hydrolysis of vegetable oils and enantioselective activity of APS-BCL by the kinetic resolution of (R,S)-1-phenylethanol with using isopropenyl acetate as an acyl donor were evaluated. An effect of reaction media with different logP values (t-butyl methyl ether, dichloromethane, diisopropyl ether, toluene, cyclohexane, n-hexane, isooctane and n-heptane) on the enantioselective activity of lipase was also studied. The high value of the enantiomeric ratio (E =308.5) with the utilization of isopropenyl acetate was achieved. Whereas, the best reaction medium turned out to be diisopropyl ether, C =47.9%, eep =98%, ees =90%, after 24 h of incubation. Moreover, the influence of ω6/ω9 polyunsaturated fatty acids (PUFAs) ratio in commercial (peanut, camelina, rape, pumpkin seed, walnut, sesame, avocado, rice, corn, black cumin, hemp, safflower, grape seed) oils was investigated for the lipase activity. For the first time, the cut-off limit of ω6/ω9 ratio was proposed. The ratio equal to or higher than 2.3 allows achieving higher lipolytic activity.

Kinetic resolution of heptan-2-ol-enantiomers by lipase-catalysed esteri�ication in organic solvent

1989 ◽  
Vol 188 (2) ◽  
pp. 144-147
Author(s):  
Karl-Heinz Engel ◽  
Martina Bohnen ◽  
Roland Tressl
Keyword(s):  
Organic Solvent ◽  
Kinetic Resolution
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