scholarly journals Ginkgo biloba extract (EGb761) did not express estrogenic activity in an immature rat uterotrophic assay

2018 ◽  
Vol 33 (3) ◽  
pp. e2018016
Author(s):  
Byeonghak Moon ◽  
Wonchan Kim ◽  
Cho Hee Park ◽  
Seung Min Oh
Keyword(s):  
Ginkgo Biloba ◽  
Estrogenic Activity ◽  
Ginkgo Biloba Extract ◽  
Immature Rat ◽  
Uterotrophic Assay

Diosgenin does not express estrogenic activity: a uterotrophic assay

2014 ◽  
Vol 92 (4) ◽  
pp. 292-298 ◽  
Author(s):  
Ivana Medigović ◽  
Nataša Ristić ◽  
Jasmina Živanović ◽  
Branka Šošić-Jurjević ◽  
Branko Filipović ◽  
...  
Keyword(s):  
Estrogen Receptor Alpha ◽  
Estrogenic Activity ◽  
Female Rats ◽  
Control Group ◽  
Immunohistochemical Expression ◽  
Vehicle Control Group ◽  
Immature Rat ◽  
Intact Control ◽  
Uterotrophic Assay ◽  
Wet Weight

This study assessed the effects of diosgenin on estrogenic activity using a uterotrophic assay. Immature female rats received diosgenin orally at doses of 200, 100, or 20 mg/kg body mass; and 17α ethynylestradiol at doses of 1 or 0.3 μg/kg, daily, for 3 consecutive days from day 19 to day 21. Controls were distributed among 2 groups: an intact control group and a vehicle control group. Animals were sacrificed 24 h after the last application of diosgenin, estradiol, or vehicle (22nd day of life). Uterine wet weight, stereological and histomorphometrical changes, immunohistochemical expression of estrogen receptor alpha (ERα), progesterone receptor (PR), and the expression of lactoferrin (LF) were examined. Diosgenin did not affect the uterine wet weight, epithelium height, volume densities of endometrium, endometrial epithelia, number of endometrial glands, or histological appearance of vaginal epithelia. ERα, PR, and LF immunostaining intensity were not altered in the animals that received diosgenin. High-potency reference ER agonist 17α-ethynylestradiol induced a significant increase in all of the measured parameters, and as expected, decreased ERα immunostaining intensity. Based on these data, it can be concluded that diosgenin, at doses of 20–200 mg/kg, did not act as an estrogen agonist in the immature rat uterotrophic assay.

Quality Control Optimization Solutions for Determination of Rutin in Supplements Containing Ginkgo Biloba Extract

2016 ◽  
Vol 12 (4) ◽  
pp. 386-390 ◽  
Author(s):  
Ivanka P. Pencheva ◽  
Vania N. Maslarska ◽  
Assena C. Stoimenova ◽  
Manoela M. Manova ◽  
Lily A. Andonova ◽  
...  
Keyword(s):  
Quality Control ◽  
Ginkgo Biloba ◽  
Ginkgo Biloba Extract ◽  
Control Optimization

Quantitative GC-FID and UHPLC-DAD Evaluation of Bioactive Compounds Extracted from Ginkgo biloba

2020 ◽  
Vol 16 (7) ◽  
pp. 893-904
Author(s):  
Alessandra von Ahn ◽  
João Henrique Z. dos Santos
Keyword(s):  
Ginkgo Biloba ◽  
Extraction Efficiency ◽  
Extraction Methods ◽  
Ginkgo Biloba Extract ◽  
Previous Method ◽  
Array Detector ◽  
Ginkgolide B ◽  
Detection Techniques ◽  
Ginkgolide A ◽  
Terpene Trilactones

Background: The official compendium of the quantification of ginkgo flavonoids from Ginkgo biloba extract has been proposed using HPLC. The drawbacks of this technique appear to be due to the restricted efficiency in terms of the recovery results and suitability of the system for the quantification of these compounds. This study investigated the potential advantages and limitations of the development of efficient extraction methods for the recovery of flavonol glycosides (quercetin, kaempferol and isorhamnetin) and terpene trilactones (bilobalide, ginkgolide A, ginkgolide B and ginkgolide C) using extraction, quantification and detection techniques, namely, GC-FID and UHPLC-DAD, which are alternatives to those techniques available in the literature. Methods: Two different extraction methodologies have been developed for the determination of flavonoids (quercetin, kaempferol and isorhamnetin) and terpene trilactones (bilobalide, ginkgolide A, ginkgolide B and ginkgolide C) using ultra-high-pressure liquid chromatography coupled to a diode array detector and gas chromatography coupled to a flame ionization detector. Results: In this study, the Ginkgo biloba extract mass, hydrolysis preparation method (with or without reflux), and volume of the extraction solution seemed to affect the ginkgo flavonoid recovery. The UHPLC-based method exhibited higher extraction efficiency for ginkgo flavonoid quantification compared to the pharmacopoeial method. The developed method exhibited higher extraction efficiency for terpene quantification compared to the previous method that used extractive solution without pH adjustment, with less time of extraction and less amount of the sample and organic solvent aliquots. Conclusion: The UHPLC and GC analysis methods established in this study are both effective and efficient. These methods may improve the quality control procedures for ginkgo extract and commercial products available in today´s natural health product market. The results indicate that redeveloped extraction methods can be a viable alternative to traditional extraction methods.

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