scholarly journals Spinal Nitric Oxide Synthase Type II Increases Neurosteroid-metabolizing Cytochrome P450c17 Expression in a Rodent Model of Neuropathic Pain

2019 ◽  
Vol 28 (4) ◽  
pp. 516-528 ◽  
Author(s):  
Sheu-Ran Choi ◽  
Alvin J Beitz ◽  
Jang-Hern Lee
Keyword(s):  
Nitric Oxide ◽  
Neuropathic Pain ◽  
Nitric Oxide Synthase ◽  
Rodent Model ◽  
Type Ii ◽  
Oxide Synthase ◽  
Cytochrome P450c17

The Role of Nitric Oxide Synthase Isoforms in Neuropathic Pain Induced by Nerve Injury in Rats

2005 ◽  
Vol 48 (1) ◽  
pp. 76
Author(s):  
Young Tae Jeon ◽  
Kwang Suk Seo ◽  
Young Jin Ro ◽  
Young Jin Lim ◽  
Seong Won Min ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Neuropathic Pain ◽  
Nitric Oxide Synthase ◽  
Nerve Injury ◽  
Oxide Synthase

Induction and cDNA sequence of inducible nitric oxide synthase from canine aortic smooth muscle cells

1998 ◽  
Vol 275 (4) ◽  
pp. H1122-H1129 ◽  
Author(s):  
Xiaofang Wang ◽  
Christopher G. A. McGregor ◽  
Virginia M. Miller
Keyword(s):  
Nitric Oxide ◽  
Smooth Muscle ◽  
Nitric Oxide Synthase ◽  
Smooth Muscle Cells ◽  
Allograft Rejection ◽  
Muscle Cells ◽  
Type Ii ◽  
Rt Pcr ◽  
Aortic Smooth Muscle ◽  
Oxide Synthase

An inducible isoform of nitric oxide synthase (type II, iNOS) is expressed in cardiac and vascular smooth muscle in response to inflammatory cytokines. The dog is an important large animal used for cardiovascular research including effects of exercise, heart failure, and allograft rejection. However, molecular probes for iNOS developed in other mammals have not been reliable for the study of iNOS induction in canine vascular smooth muscle. Experiments were designed to develop a molecular probe for canine iNOS. Smooth muscle cells were isolated from canine aortas. The cells ( passages 3–10) were incubated for 1, 3, 6, 12, 24, 48, or 72 h in the absence and presence of Escherichia coli lipopolysaccharide (LPS) to induce iNOS. Total RNA was isolated from the cells using standard techniques. RT-PCR with primers against conserved regions of all known iNOS enzyme was used to clone the iNOS cDNA. RT-PCR showed a single band only from cells treated with LPS. Cloned cDNA from cultured canine aortic smooth muscle cells has 84% homology to human, 81% to rat, and 81% to mouse iNOS gene. Identification of the cDNA for canine iNOS will be useful in the study of differential, transcriptional regulation of inducible (type II) compared with constitutive endothelial (type III) NOS in canine studies of allograft rejection and cardiovascular disease.

scholarly journals P2X7 nucleotide receptor activation enhances IFNγ-induced type II nitric oxide synthase activity in BV-2 microglial cells

2003 ◽  
Vol 87 (2) ◽  
pp. 344-352 ◽  
Author(s):  
Fernand-Pierre Gendron ◽  
Malgorzata Chalimoniuk ◽  
Joanna Strosznajder ◽  
Siming Shen ◽  
Fernando A. González ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Receptor Activation ◽  
Microglial Cells ◽  
Type Ii ◽  
Nucleotide Receptor ◽  
Oxide Synthase ◽  
Nitric Oxide Synthase Activity

scholarly journals Protein kinases in induction of type II nitric oxide synthase

1996 ◽  
Vol 71 ◽  
pp. 6
Author(s):  
Hideki Moritoki ◽  
Tetsuhiro Hisayama ◽  
Wataru Kondoh ◽  
Kann Kida
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Protein Kinases ◽  
Type Ii ◽  
Oxide Synthase

Inducible (type II) nitric oxide synthase in human placental villous tissue of normotensive, pre-eclamptic and intrauterine growth-restricted pregnancies

Placenta ◽  
1997 ◽  
Vol 18 (4) ◽  
pp. 261-268 ◽  
Author(s):  
L. Myatt ◽  
A.L.W. Eis ◽  
D.E. Brockman ◽  
W. Kossenjans ◽  
I. Greer ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Type Ii ◽  
Intrauterine Growth ◽  
Oxide Synthase ◽  
Villous Tissue

scholarly journals Effects of Hyperbaric Oxygen on Pain-Related Behaviours and Nitric Oxide Synthase Expression in a Rat Model of Neuropathic Pain

2013 ◽  
Vol 18 (3) ◽  
pp. 137-141 ◽  
Author(s):  
Guang Han ◽  
Lu Li ◽  
Ling-xin Meng
Keyword(s):  
Nitric Oxide ◽  
Spinal Cord ◽  
Neuropathic Pain ◽  
Nitric Oxide Synthase ◽  
Hyperbaric Oxygen ◽  
Rat Model ◽  
Withdrawal Threshold ◽  
Withdrawal Latency ◽  
Oxide Synthase ◽  
Inducible Nos

BACKGROUND: Neuropathic pain is complex, and a satisfactory therapeutic method of treatment has yet to be developed; therefore, finding a new and effective therapeutic method is an important issue in the field of neuropathic pain.OBJECTIVE: To determine the effects of hyperbaric oxygen (HBO) on pain-related behaviours and nitric oxide synthase (NOS) expression in a rat model of neuropathic pain.METHODS: Forty male Sprague Dawley rats were randomly divided into five groups (eight rats per group) including control, sham operation, sciatic nerve with chronic constriction injury (CCI), HBO pretreatment (pre-HBO) and HBO post-treatment (post-HBO) groups. Pain-related behaviours and NOS expression in the spinal cord were compared among the five groups.RESULTS: Compared with the CCI group, the mechanical withdrawal threshold was significantly increased and thermal withdrawal latency was significantly extended in the pre-HBO and post-HBO groups (all P<0.05). After CCI, expression of spinal neuronal NOS and inducible NOS were increased. Expression of spinal neuronal NOS and inducible NOS were significantly decreased in the pre-HBO and post-HBO groups compared with the CCI group (all P<0.05). Spinal eNOS expression changed very little.DISCUSSION: HBO has been used as an effective and noninvasive method for the treatment of spinal cord injuries and high-altitude sickness, and in immunosuppression and stem-cell research; however, it has yet to be applied to the treatment of neuropathic pain. The present study indicated that HBO effectively increased mechanical withdrawal threshold and thermal withdrawal latency, demonstrating that HBO has therapeutic effects on neuropathic pain.CONCLUSION: HBO inhibits pain in rats with CCI through the regulation of spinal NOS expression.

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