scholarly journals FxClear, A Free-hydrogel Electrophoretic Tissue Clearing Method for Rapid De-lipidation of Tissues with High Preservation of Immunoreactivity

2019 ◽  
Vol 28 (3) ◽  
pp. 436-445
Author(s):  
Jungyoon Choi ◽  
Eunsoo Lee ◽  
June Hoan Kim ◽  
Woong Sun
Keyword(s):  
Tissue Clearing ◽  
Clearing Method

scholarly journals A Hydrophobic Tissue Clearing Method for Rat Brain Tissue

10.3791/61821 ◽  
2020 ◽  
Author(s):  
Kristin N. Kirchner ◽  
Hailong Li ◽  
Adam R. Denton ◽  
Steven B. Harrod ◽  
Charles F. Mactutus ◽  
...  
Keyword(s):  
Rat Brain ◽  
Brain Tissue ◽  
Tissue Clearing ◽  
Clearing Method ◽  
Rat Brain Tissue

scholarly journals EZ Clear for simple, rapid, and robust mouse whole organ clearing

2022 ◽  
Author(s):  
Chih-Wei Hsu ◽  
Juan Cerda ◽  
Jason M Kirk ◽  
Williamson D. Turner ◽  
Tara L. Rasmussen ◽  
...  
Keyword(s):  
Biomedical Research ◽  
Room Temperature ◽  
Adult Mouse ◽  
Dimensional Space ◽  
Three Dimensional ◽  
Fluorescence Signal ◽  
Tissue Architecture ◽  
Tissue Clearing ◽  
Clearing Method ◽  
Three Dimensional Space

Tissue clearing for whole organ cell profiling has revolutionized biology and imaging for exploration of organs in three-dimensional space without compromising tissue architecture. But complicated, laborious procedures, or expensive equipment, as well as the use of hazardous, organic solvents prevents the widespread adoption of these methods. Here we report a simple and rapid tissue clearing method, EZ Clear, that can clear whole adult mouse organs in 48 hours in just three simple steps. Samples stay at room temperature and remain hydrated throughout the clearing process, preserving endogenous and synthetic fluorescence, without altering sample size. After wholemount clearing and imaging, EZ Cleared samples can be further processed for downstream embedding and cryosectioning followed by standard histology or immunostaining, without loss of endogenous or synthetic fluorescence signal. Overall, the simplicity, speed, and flexibility of EZ Clear make it easy to adopt and apply to diverse approaches in biomedical research.

scholarly journals Visualization of the Retina in Intact Eyes of Mice and Ferrets Using a Tissue Clearing Method

2020 ◽  
Vol 9 (3) ◽  
pp. 1 ◽  
Author(s):  
Yunyan Ye ◽  
Tung Anh Dinh Duong ◽  
Kengo Saito ◽  
Yohei Shinmyo ◽  
Yoshie Ichikawa ◽  
...  
Keyword(s):  
Tissue Clearing ◽  
Clearing Method

scholarly journals A pH-Adjustable Tissue Clearing Solution That Preserves Lipid Ultrastructures: Suitable Tissue Clearing Method for DDS Evaluation

Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1070
Author(s):  
Shintaro Fumoto ◽  
Eriko Kinoshita ◽  
Keisuke Ohta ◽  
Kei-ichiro Nakamura ◽  
Tasuku Hirayama ◽  
...  
Keyword(s):  
Spatial Distribution ◽  
Three Dimensional ◽  
Fluorescent Intensity ◽  
Tissue Clearing ◽  
Carbocyanine Dyes ◽  
Electron Microscopy Level ◽  
Wide Ph Range ◽  
Ph Range ◽  
Clearing Method ◽  
And Oxidative Stress

Visualizing biological events and states to resolve biological questions is challenging. Tissue clearing permits three-dimensional multicolor imaging. Here, we describe a pH-adjustable tissue clearing solution, Seebest (SEE Biological Events and States in Tissues), which preserves lipid ultrastructures at an electron microscopy level. Adoption of polyethylenimine was required for a wide pH range adjustment of the tissue clearing solution. The combination of polyethylenimine and urea had a good tissue clearing ability for multiple tissues within several hours. Blood vessels stained with lipophilic carbocyanine dyes were deeply visible using the solution. Adjusting the pH of the solution was important to maximize the fluorescent intensity and suppress dye leakage during tissue clearing. The spatial distribution of doxorubicin and oxidative stress were observable using the solution. Moreover, spatial distribution of liposomes in the liver was visualized. Hence, the Seebest solution provides pH-adjustable, rapid, sufficient tissue clearing, while preserving lipid ultrastructures, which is suitable for drug delivery system evaluations.

Using a new three-dimensional CUBIC tissue-clearing method to examine the brain during experimental cerebral malaria

2021 ◽  
Author(s):  
Julia Matsuo-Dapaah ◽  
Michelle Sue Jann Lee ◽  
Ken J Ishii ◽  
Kazuki Tainaka ◽  
Cevayir Coban
Keyword(s):  
Olfactory Bulb ◽  
Cerebral Malaria ◽  
Fluorescent Microscopy ◽  
Three Dimensions ◽  
Whole Brain ◽  
Cerebral Microvessels ◽  
Experimental Cerebral Malaria ◽  
Tissue Clearing ◽  
The Brain ◽  
Clearing Method

Abstract Cerebral malaria (CM) is a life-threatening complication of the malaria disease caused by Plasmodium falciparum infection and is responsible for the death of half a million people annually. The molecular pathogenesis underlying CM in humans is not completely understood, although sequestration of infected erythrocytes in cerebral microvessels is thought to play a major role. In contrast, experimental cerebral malaria (ECM) models in mice have been thought to be distinct from human CM, and are mainly caused by inflammatory mediators. Here, to understand the spatial distribution and the potential sequestration of parasites in the whole-brain microvessels during a mouse model of ECM, we utilized the new tissue-clearing method CUBIC (Clear, Unobstructed, Brain/Body Imaging Cocktails and Computational analysis) with light sheet fluorescent microscopy (LSFM), and reconstructed images in three-dimensions (3D). We demonstrated significantly greater accumulation of Plasmodium berghei ANKA (PbANKA) parasites in the olfactory bulb of mice, compared with the other parts of the brain, including the cerebral cortex, cerebellum, and brainstem. Furthermore, we show that PbANKA parasites preferentially accumulate in the brainstem when the olfactory bulb is surgically removed. This study therefore not only highlights a successful application of CUBIC tissue-clearing technology to visualize the whole brain and its microvessels during ECM, but it also shows CUBIC’s future potential for visualizing pathological events in the whole ECM brain at the cellular level, an achievement that would greatly advance our understanding of human cerebral malaria.

scholarly journals Advantages of Binaree tissue clearing method

IBRO Reports ◽  
2019 ◽  
Vol 6 ◽  
pp. S521
Author(s):  
Eun Shil Lee ◽  
Eunjoo Lee
Keyword(s):  
Tissue Clearing ◽  
Clearing Method
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