scholarly journals The role of the collagen type 1 alpha 1 gene polymorphism in the prediction of osteoporosis and fracture risk in Turkish postmenopausal women

2016 ◽  
Vol 62 (1) ◽  
pp. 1-8
Author(s):  
İlknur Saban
Keyword(s):  
Gene Polymorphism ◽  
Fracture Risk ◽  
Postmenopausal Women ◽  
Collagen Type ◽  
Collagen Type 1

scholarly journals A Rare Cause of Protrusio Acetabuli: Osteoporosis Associated with Collagen Type 1 Alpha 1 Gene Polymorphism

2014 ◽  
Vol 20 (3) ◽  
pp. 135-136
Author(s):  
Pelin Oktayoğlu ◽  
Belkıs Aydınol ◽  
Mehmet Çağlayan ◽  
Mehtap Bozkurt ◽  
Kemal Nas
Keyword(s):  
Gene Polymorphism ◽  
Collagen Type ◽  
Protrusio Acetabuli ◽  
Collagen Type 1

scholarly journals Knockdown of Microrna-1260 Promotes Up-Regulation of Both Collagen Type 1 Alpha 1 and Monocyte Chemoattractant Protein-1 in Vascular Smooth Muscle Cells from Human Abdominal Aortic Tissues

2020 ◽  
Vol 7 (1) ◽  
pp. 1103-1103
Author(s):  
Crystal Yin Tung CHAN ◽  
Bernice Lai Yee CHEUK ◽  
Stephen Wing Keung CHENG
Keyword(s):  
Smooth Muscle ◽  
Matrix Metalloproteinase ◽  
Collagen Type ◽  
Monocyte Chemoattractant Protein 1 ◽  
Monocyte Chemoattractant ◽  
Monocyte Chemoattractant Protein ◽  
Abdominal Aortic ◽  
Collagen Type 1

Objectives: MicroRNA (miR)-1260 was identified to be down-regulated in vascular smooth muscle cells (VSMCs) from human abdominal aortic aneurysm (AAA) tissues in our previous microarray profiling assay. MiR-1260 was predicted to target and down-regulate collagen type 1 alpha 1 (COL1A1), which is closely related to AAA formation, from a bioinformatics analysis. However, the role of miR-1260 in VSMCs for AAA formation still remains uncertain. This study aims to investigate the role of miR-1260 in human VSMCs. Methods: Stable overexpression and knockdown of miR-1260 using lentivirus were performed in VSMCs cultured from human abdominal aortic tissues. Expression of COL1A1 protein was investigated, followed by investigating the expressions of several key components involved in AAA pathogenic features: monocyte chemoattractant protein-1 (MCP-1) for inflammation; matrix metalloproteinase (MMP)-2, MMP-9, tissue inhibitor of matrix metalloproteinase (TIMP)- 1 and TIMP-2 for elastin fragmentation. Apoptosis of VSMCs was also examined. Results: Both COL1A1 and MCP-1 were significantly down-regulated upon miR-1260 overexpression, whereas they were significantly up-regulated upon miR-1260 knockdown. Neither protein expressions of MMP-2, MMP-9, TIMP-1 and TIMP-2 nor apoptosis were significantly different between miR-1260 overexpression/knockdown and corresponding controls. Conclusions: Our findings suggested suppression of miR-1260, which was previously found associated with AAA VSMCs, may promote up-regulation of COL1A1 and MCP-1 in human VSMCs, possibly promoting compensatory collagen synthesis and inflammation for AAA formation.

scholarly journals Direct comparison of 3D and 2D cultivation reveals higher osteogenic capacity of elderly osteoblasts in 3D

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Stephan Payr ◽  
Elizabeth Rosado-Balmayor ◽  
Thomas Tiefenboeck ◽  
Tim Schuseil ◽  
Marina Unger ◽  
...  
Keyword(s):  
Gene Expression ◽  
Collagen Type ◽  
3D Culture ◽  
Osteogenic Potential ◽  
Donor Age ◽  
Collagen Type 1 ◽  
2D And 3D ◽  
Human Primary Osteoblasts ◽  
Gene Expression Levels

Abstract Background The aim of this study was the investigation of the osteogenic potential of human osteoblasts of advanced donor age in 2D and 3D culture. Methods Osteoblasts were induced to osteogenic differentiation and cultivated, using the same polystyrene material in 2D and 3D culture for 2 weeks. Samples were taken to evaluate alkaline phosphatase (ALP) activity, mineralization and gene expression. Results Osteoprotegerin (OPG) levels were significantly increased (8.2-fold) on day 7 in 3D compared to day 0 (p < 0.0001) and 11.6-fold higher in 3D than in 2D (p < 0.0001). Both culture systems showed reduced osteocalcin (OC) levels (2D 85% and 3D 50% of basic value). Collagen type 1 (Col1) expression was elevated in 3D on day 7 (1.4-fold; p = 0.009). Osteopontin (OP) expression showed 6.5-fold higher levels on day 7 (p = 0.002) in 3D than in 2D. Mineralization was significantly higher in 3D on day 14 (p = 0.0002). Conclusion Advanced donor age human primary osteoblasts reveal significantly higher gene expression levels of OPG, Col1 and OP in 3D than in monolayer. Therefore, it seems that a relatively high potential of bone formation in a natural 3D arrangement is presumably still present in osteoblasts of elderly people. Trial registration 5217/11 on the 22nd of Dec. 2011.

scholarly journals Evaluation of Collagen Type-1 Production and Anti-Inflammatory Activities of Human Placental Extracts in Human Gingival Fibroblasts

2016 ◽  
Vol 25 (3) ◽  
pp. 277-281 ◽  
Author(s):  
Homare Akagi ◽  
Yasuhiro Imamura ◽  
Yoshimasa Makita ◽  
Hiroe Nakamura ◽  
Naomi Hasegawa ◽  
...  
Keyword(s):  
Collagen Type ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Anti Inflammatory ◽  
Collagen Type 1
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