scholarly journals Analysis of nanomechanical properties of Borrelia burgdorferi spirochetes under the influence of lytic factors in an in vitro model using atomic force microscopy

2015 ◽  
Vol 69 ◽  
pp. 1222-1227 ◽  
Author(s):  
Małgorzata Tokarska-Rodak ◽  
Maria Kozioł-Montewka ◽  
Krzysztof Skrzypiec ◽  
Tomasz Chmielewski ◽  
Ewaryst Mendyk ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Borrelia Burgdorferi ◽  
In Vitro Model ◽  
Force Microscopy ◽  
Nanomechanical Properties ◽  
Atomic Force ◽  
Vitro Model

scholarly journals Nanostructural and mechanical changes in the sclera following proteoglycan depletion

2018 ◽  
Vol 2 (2) ◽  
pp. 14-17
Author(s):  
Zhuola Zhuola ◽  
Steve Barrett ◽  
Yalda Ashraf Kharaz ◽  
Riaz Akhtar
Keyword(s):  
Mechanical Properties ◽  
Collagen Fibril ◽  
Enzymatic Degradation ◽  
Ocular Tissues ◽  
Force Microscopy ◽  
Nanomechanical Properties ◽  
Atomic Force ◽  
Fibril Morphology

The mechanical properties of ocular tissues, such as the sclera, have a major impact on healthy eye function, and are governed by the properties and composition of the microstructural components. For example, biomechanical degradation associated with myopia occurs alongside a reduction of proteoglycans (PGs). In this study, the role of PG degradation in the nanomechanical properties of the porcine sclera is explored. In-vitro enzymatic degradation of PGs was conducted with α-amylase and chondroitinase ABC enzymes. Collagen fibril morphology and nanomechanical stiffness were measured with atomic force microscopy (AFM). The elastic modulus of the tissue was reduced in all enzyme-treated samples relative to controls. In addition, collagen fibril organization was disrupted by PG depletion. Our data demonstrate that PGs play an important role in determining not only the mechanical properties at these length scales, but also collagen fibril arrangement.

scholarly journals Visualization by atomic force microscopy of tobacco mosaic virus movement protein–RNA complexes formed in vitro

2001 ◽  
Vol 82 (6) ◽  
pp. 1503-1508 ◽  
Author(s):  
O. I. Kiselyova ◽  
I. V. Yaminsky ◽  
E. M. Karger ◽  
O. Yu. Frolova ◽  
Y. L. Dorokhov ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Movement Protein ◽  
Structural Conversion ◽  
Force Microscopy ◽  
Rna Transcripts ◽  
Atomic Force ◽  
Rna Complexes

The structure of complexes formed in vitro by tobacco mosaic virus (TMV)-coded movement protein (MP) with TMV RNA and short (890 nt) synthetic RNA transcripts was visualized by atomic force microscopy on a mica surface. MP molecules were found to be distributed along the chain of RNA and the structure of MP–RNA complexes depended on the molar MP:RNA ratios at which the complexes were formed. A rise in the molar MP:TMV RNA ratio from 20:1 to 60–100:1 resulted in an increase in the density of the MP packaging on TMV RNA and structural conversion of complexes from RNase-sensitive ‘beads-on-a-string’ into a ‘thick string’ form that was partly resistant to RNase. The ‘thick string’-type RNase-resistant complexes were also produced by short synthetic RNA transcripts at different MP:RNA ratios. The ‘thick string’ complexes are suggested to represent clusters of MP molecules cooperatively bound to discrete regions of TMV RNA and separated by protein-free RNA segments.

Nanomechanical properties of lithiated Si nanowires probed with atomic force microscopy

2012 ◽  
Vol 45 (27) ◽  
pp. 275301 ◽  
Author(s):  
Hyunsoo Lee ◽  
Weonho Shin ◽  
Jang Wook Choi ◽  
Jeong Young Park
Keyword(s):  
Atomic Force Microscopy ◽  
Si Nanowires ◽  
Force Microscopy ◽  
Nanomechanical Properties ◽  
Atomic Force

In situ nanomechanical properties of natural oil bodies studied using atomic force microscopy

2020 ◽  
Vol 570 ◽  
pp. 362-374 ◽  
Author(s):  
Nan Yang ◽  
Chunxia Su ◽  
Yuemei Zhang ◽  
Junji Jia ◽  
Robert L. Leheny ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Oil Bodies ◽  
Force Microscopy ◽  
Nanomechanical Properties ◽  
Atomic Force ◽  
Natural Oil

scholarly journals Different directional energy dissipation of heterogeneous polymers in bimodal atomic force microscopy

RSC Advances ◽  
2019 ◽  
Vol 9 (47) ◽  
pp. 27464-27474 ◽  
Author(s):  
Xinfeng Tan ◽  
Dan Guo ◽  
Jianbin Luo
Keyword(s):  
Atomic Force Microscopy ◽  
Energy Dissipation ◽  
Dynamic Force ◽  
Force Detection ◽  
Force Microscopy ◽  
Nanomechanical Properties ◽  
Powerful Technique ◽  
Atomic Force ◽  
Nanoscale Imaging ◽  
Heterogeneous Polymers

Dynamic force microscopy (DFM) has become a multifunctional and powerful technique for the study of the micro–nanoscale imaging and force detection, especially in the compositional and nanomechanical properties of polymers.

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