scholarly journals The immunoexpression of androgen receptor, estrogen receptors alpha and beta, vanilloid type 1 receptor and cytochrome p450 aromatase in rats testis chronically treated with letrozole, an aromatase inhibitor

2014 ◽  
Vol 52 (3) ◽  
pp. 206-217 ◽  
Author(s):  
Anna Pilutin ◽  
Kamila Misiakiewicz-Has ◽  
Agnieszka Kolasa ◽  
Irena Baranowska-Bosiacka ◽  
Mariola Marchlewicz ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Androgen Receptor ◽  
Estrogen Receptors ◽  
Aromatase Inhibitor ◽  
P450 Aromatase ◽  
Cytochrome P450 Aromatase

scholarly journals Effect of Estradiol and Nonylphenol on mRNA Expression of Estrogen Receptors α and β, and Cytochrome P450 Aromatase in the Gonad of Chicken Embryos

2002 ◽  
Vol 39 (4) ◽  
pp. 302-309 ◽  
Author(s):  
Masanori Sakimura ◽  
Akira Tsukada ◽  
Makoto Usami ◽  
Shozo Hanzawa ◽  
Noboru Saito ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Estrogen Receptors ◽  
Mrna Expression ◽  
Chicken Embryos ◽  
P450 Aromatase ◽  
Cytochrome P450 Aromatase

Expression of 17β-hydroxysteroid dehydrogenase in human granulosa cells: correlation with follicular size, cytochrome P450 aromatase activity and oestradiol production

1994 ◽  
Vol 143 (1) ◽  
pp. 139-150 ◽  
Author(s):  
S A Ghersevich ◽  
M H Poutanen ◽  
H K Martikainen ◽  
R K Vihko
Keyword(s):  
Cytochrome P450 ◽  
Granulosa Cells ◽  
Protein Concentration ◽  
Hydroxysteroid Dehydrogenase ◽  
Enzyme Protein ◽  
P450 Aromatase ◽  
Human Granulosa Cells ◽  
Cytochrome P450 Aromatase ◽  
In Cells

Abstract The aim of this study was to examine the expression and regulation of type 1 17β-hydroxysteroid dehydrogenase (type 1 17-HSD) enzyme protein and mRNA, and 17-HSD activity in human granulosa cells. The cells were obtained from patients taking part in an in vitro fertilization programme. The cells from each patient were divided into two groups: cells obtained from preovulatory follicles (LGC=granulosa cells from large follicles ≥ 18 mm in diameter), and cells from other visible follicles (SGC=granulosa cells from small follicles, less than 15 mm in diameter). The identity of 17-HSD enzyme protein expressed in human granulosa cells with placental cytosolic 17-HSD (type 1 17-HSD) was assessed by immunoblot analysis using polyclonal antibodies, and the enzyme was immunolocalized in the cytoplasm of granulosa cells. Type 1 17-HSD protein concentration, 17-HSD and cytochrome P450 aromatase (P450arom) activities and oestradiol (OE2) production in cells from LGC were significantly lower than the corresponding values obtained in SGC in the same patient (paired t-test). The type 1 17-HSD protein concentration, 17-HSD activity and P450arom activity were 140±16% (mean±s.e.m.), 121±22% and 113±26% higher in cells from SGC, which was also reflected in a 70±12% higher OE2 production in these cells. In freshly isolated cells from LGC or SGC, a high correlation between 17-HSD and P450arom activities was observed (r=0·93, P<0·001). In long-term cultured cells, type 1 17-HSD was stably expressed at least until day 9, while P450arom expression decreased. In addition, treatments with gonadotrophins did not affect type 1 17-HSD protein concentration and 17-HSD activity. In contrast to this, both P450arom activity and OE2 production were significantly increased (P<0·05). The data, therefore, suggest that type 1 17-HSD and P450arom are expressed in parallel during the latest stages of follicular maturation but, in cultured granulosa-luteal cells, the enzymes are regulated by distinct mechanisms. Journal of Endocrinology (1994) 143, 139–150

Closely Related Genes Encode Developmental and Tissue Isoforms of Porcine Cytochrome P450 Aromatase

1997 ◽  
Vol 16 (6) ◽  
pp. 769-777 ◽  
Author(s):  
INHO CHOI ◽  
DERYL L. TROYER ◽  
DEAN L. CORNWELL ◽  
KATHY R. KIRBY-DOBBELS ◽  
WERNER R. COLLANTE ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
P450 Aromatase ◽  
Cytochrome P450 Aromatase

scholarly journals Characterization of Two Types of Cytochrome P450 Aromatase in the Serial-sex Changing Gobiid Fish, Trimma okinawae

2004 ◽  
Vol 21 (4) ◽  
pp. 417-425 ◽  
Author(s):  
Yasuhisa Kobayashi ◽  
Tohru Kobayashi ◽  
Masaru Nakamura ◽  
Tomoki Sunobe ◽  
Craig E. Morrey ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Gobiid Fish ◽  
P450 Aromatase ◽  
Cytochrome P450 Aromatase
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