scholarly journals Electrochemical sensing platform for simultaneous detection of 6-mercaptopurine and 6-thioguanine using RGO-Cu2O/Fe2O3 modified screen-printed graphite electrode

2021 ◽  
Author(s):  
Fatemeh Irannezhad ◽  
Jamileh Seyed-Yazdi ◽  
Seyedeh Hoda Hekmatara
Keyword(s):  
Dynamic Range ◽  
Graphite Electrode ◽  
Limit Of Detection ◽  
Simultaneous Detection ◽  
Differential Pulse ◽  
Electrochemical Sensing ◽  
X Ray Diffraction ◽  
Sensing Platform ◽  
Facile Fabrication ◽  
Screen Printed

A sensitive electrochemical sensor was developed using reduced graphene oxide RGO-Cu2O/Fe2O3 nanocomposite for 6-mercaptopurine detection based on a facile fabrication method. The surface morphology and structural composition of this nanocomposite was evaluated by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and Fourier transform infrared (FT-IR) spectroscopy. The screen-printed graphite electrode (SPGE) modified with RGO-Cu2O/Fe2O3 nanocomposite (RGO-Cu2O/Fe2O3/SPGE) indicated excellent electrochemical properties to detect 6-mercaptopurine. The linear dynamic range was estimated between 0.05 and 400.0 μM for 6-mercaptopurine detection, with a limit of detection of 0.03 μM. Also, RGO-Cu2O/Fe2O3/SPGE sensor showed good activity for simultaneous detection of 6-mercaptopurine and 6-thioguanine. In the coexistence system of 6-mercaptopurine and 6-thioguanine, two clear and well-isolated voltammetric peaks were obtained by differential pulse voltammetry (DPV). Additionally, the proposed sensor was examined for applicability by determining 6-mercaptopurine and 6-thioguanine in real samples, and the recovery in the range of 97.5-103.0 % was obtained.

Magnetically induced self-assembly DNAzyme electrochemical biosensor based on gold-modified α-Fe2O3/Fe3O4 heterogeneous nanoparticles for sensitive detection of Ni2+

2021 ◽  
Author(s):  
Lulu Yu ◽  
Min Liu ◽  
Yanling Zhang ◽  
Yun Ni ◽  
Shaobo Wu ◽  
...  
Keyword(s):  
Self Assembly ◽  
Electrochemical Biosensor ◽  
Limit Of Detection ◽  
Sol Gel ◽  
Trisodium Citrate ◽  
Thiol Group ◽  
Differential Pulse ◽  
Electrochemical Sensing ◽  
Detection Range ◽  
Sensing Platform

Abstract A magnetically induced self-assembly DNAzyme electrochemical biosensor based on gold-modified α-Fe2O3/Fe3O4 heterogeneous nanoparticles was successfully fabricated to detect Nickel(II) (Ni2+). The phase composition and magnetic properties of α-Fe2O3/Fe3O4 heterogeneous nanoparticles controllably prepared by the citric acid (CA) sol-gel method were investigated in detail. The α-Fe2O3/Fe3O4 heterogeneous nanoparticles were modified by using trisodium citrate as reducing agent, and the magnetically induced self-assembly α-Fe2O3/Fe3O4-Au nanocomposites were obtained. The designed Ni2+-dependent DNAzyme consisted of the catalytic chain modified with the thiol group (S1-SH) and the substrate chain modified with methylene blue (S2-MB). The MGCE/α-Fe2O3/Fe3O4-Au/S1/BSA/S2 electrochemical sensing platform was constructed and differential pulse voltammetry (DPV) was applied for electrochemical detection. Under the optimum experimental parameters, the detection range of the biosensor was 100 pM-10 µM (R2= 0.9978) with the limit of detection (LOD) of 55 pM. The biosensor had high selectivity, acceptable stability, and reproducibility (RSD = 4.03%).

scholarly journals Paper-based aptamer-antibody biosensor for gluten detection in a deep eutectic solvent (DES)

2021 ◽  
Author(s):  
Rossella Svigelj ◽  
Nicolò Dossi ◽  
Cristian Grazioli ◽  
Rosanna Toniolo
Keyword(s):  
Dynamic Range ◽  
Limit Of Detection ◽  
Low Cost ◽  
Deep Eutectic Solvent ◽  
Electrochemical Sensing ◽  
Sensing Platform ◽  
Screen Printed Carbon Electrode ◽  
Highly Sensitive ◽  
Rapid Tests

AbstractPaper has been widely employed as cheap material for the development of a great number of sensors such as pregnancy tests, strips to measure blood sugar, and COVID-19 rapid tests. The need for new low-cost analytical devices is growing, and consequently the use of these platforms will be extended to different assays, both for the final consumer and within laboratories. This work describes a paper-based electrochemical sensing platform that uses a paper disc conveniently modified with recognition molecules and a screen-printed carbon electrode (SPCE) to achieve the detection of gluten in a deep eutectic solvent (DES). This is the first method coupling a paper biosensor based on aptamers and antibodies with the DES ethaline. Ethaline proved to be an excellent extraction medium allowing the determination of very low gluten concentrations. The biosensor is appropriate for the determination of gluten with a limit of detection (LOD) of 0.2 mg L−1 of sample; it can detect gluten extracted in DES with a dynamic range between 0.2 and 20 mg L−1 and an intra-assay coefficient of 10.69%. This approach can be of great interest for highly gluten-sensitive people, who suffer from ingestion of gluten quantities well below the legal limit, which is 20 parts per million in foods labeled gluten-free and for which highly sensitive devices are essential. Graphical abstract

scholarly journals Simultaneous electrochemical sensing of dihydroxy benzene isomers at cost-effective allura red polymeric film modified glassy carbon electrode

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Pattan-Siddappa Ganesh ◽  
Ganesh Shimoga ◽  
Seok-Han Lee ◽  
Sang-Youn Kim ◽  
Eno E. Ebenso
Keyword(s):  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Limit Of Detection ◽  
Tap Water ◽  
Differential Pulse ◽  
Oxidation Potential ◽  
Electrochemical Sensing ◽  
Carbon Electrode ◽  
Buffer Solution ◽  
Allura Red

Abstract Background A simple and simultaneous electrochemical sensing platform was fabricated by electropolymerization of allura red on glassy carbon electrode (GCE) for the interference-free detection of dihydroxy benzene isomers. Methods The modified working electrode was characterized by electrochemical and field emission scanning electron microscopy methods. The modified electrode showed excellent electrocatalytic activity for the electrooxidation of catechol (CC) and hydroquinone (HQ) at physiological pH of 7.4 by cyclic voltammetric (CV) and differential pulse voltammetric (DPV) techniques. Results The effective split in the overlapped oxidation signal of CC and HQ was achieved in a binary mixture with peak to peak separation of 0.102 V and 0.103 V by CV and DPV techniques. The electrode kinetics was found to be adsorption-controlled. The oxidation potential directly depends on the pH of the buffer solution, and it witnessed the transfer of equal number of protons and electrons in the redox phenomenon. Conclusions The limit of detection (LOD) for CC and HQ was calculated to be 0.126 μM and 0.132 μM in the linear range of 0 to 80.0 μM and 0 to 110.0 μM, respectively, by ultra-sensitive DPV technique. The practical applicability of the proposed sensor was evaluated for tap water sample analysis, and good recovery rates were observed. Graphical abstract Electrocatalytic interaction of ALR/GCE with dihydroxy benzene isomers.

scholarly journals An efficient electrochemical sensing of hazardous catechol and hydroquinone at direct green 6 decorated carbon paste electrode

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
K. Chetankumar ◽  
B. E. Kumara Swamy ◽  
S. C. Sharma ◽  
S. A. Hariprasad
Keyword(s):  
Carbon Paste Electrode ◽  
Limit Of Detection ◽  
Tap Water ◽  
Differential Pulse ◽  
Electrochemical Sensing ◽  
Carbon Paste ◽  
Pulse Voltammetry ◽  
Two Peaks ◽  
Paste Electrode ◽  
Scanning Electron

AbstractIn this proposed work, direct green 6 (DG6) decorated carbon paste electrode (CPE) was fabricated for the efficient simultaneous and individual sensing of catechol (CA) and hydroquinone (HY). Electrochemical deeds of the CA and HY were carried out by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) at poly-DG6-modfied carbon paste electrode (Po-DG6-MCPE). Using scanning electron microscopy (SEM) studied the surface property of unmodified CPE (UCPE) and Po-DG6-MCPE. The decorated sensor displayed admirable electrocatalytic performance with fine stability, reproducibility, selectivity, low limit of detection (LLOD) for HY (0.11 μM) and CC (0.09 μM) and sensor process was originated to be adsorption-controlled phenomena. The Po-DG6-MCPE sensor exhibits well separated two peaks for HY and CA in CV and DPV analysis with potential difference of 0.098 V. Subsequently, the sensor was practically applied for the analysis in tap water and it consistent in-between for CA 93.25–100.16% and for HY 97.25–99.87% respectively.

scholarly journals An Electrochemical Strategy for the Simultaneous Detection of Doxorubicin and Simvastatin for Their Potential Use in the Treatment of Cancer

Biosensors ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 15
Author(s):  
Iulia Rus ◽  
Mihaela Tertiș ◽  
Cristina Barbălată ◽  
Alina Porfire ◽  
Ioan Tomuță ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Electrolyte Solution ◽  
Electrode Material ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Simultaneous Detection ◽  
Linear Sweep Voltammetry ◽  
Pharmaceutical Formulations ◽  
Promising Tool ◽  
Scan Rate

The aim of this study was to develop a disposable, simple, fast, and sensitive sensor for the simultaneous electrochemical detection of doxorubicin (DOX) and simvastatin (SMV), which could be used in preclinical studies for the development of new pharmaceutical formulations for drug delivery. Firstly, the electrochemical behavior of each molecule was analyzed regarding the influence of electrode material, electrolyte solution, and scan rate. After this, the proper electrode material, electrolyte solution, and scan rate for both active substances were chosen, and a linear sweep voltammetry procedure was optimized for simultaneous detection. Two chronoamperometry procedures were tested, one for the detection of DOX in the presence of SMV, and the other one for the detection of DOX and SMV together. Finally, calibration curves for DOX and SMV in the presence of each other were obtained using both electrochemical methods and the results were compared. The use of amperometry allowed for a better limit of detection (DOX: 0.1 μg/mL; SMV: 0.7 μg/mL) than the one obtained in voltammetry (1.5 μg/mL for both drugs). The limits of quantification using amperometry were 0.5 μg/mL for DOX (dynamic range: 0.5–65 μg/mL) and 2 μg/mL for SMV (dynamic range: 2–65 μg/mL), while using voltammetry 1 μg/mL was obtained for DOX (dynamic range: 1–100 μg/mL) and 5 μg/mL for SMV (dynamic range: 5–100 μg/mL). This detection strategy represents a promising tool for the analysis of new pharmaceutical formulations for targeted drug delivery containing both drugs, whose association was proven to bring benefits in the treatment of cancer.

scholarly journals Surface-Enhanced Oxidation and Determination of Isothipendyl Hydrochloride at an Electrochemical Sensing Film Constructed by Multiwalled Carbon Nanotubes

2012 ◽  
Vol 2012 ◽  
pp. 1-6
Author(s):  
S. N. Prashanth ◽  
Shankara S. Kalanur ◽  
Nagappa L. Teradal ◽  
J. Seetharamappa
Keyword(s):  
Limit Of Detection ◽  
Biological Fluids ◽  
Differential Pulse ◽  
Electrochemical Sensing ◽  
Good Precision ◽  
Multiwalled Carbon ◽  
Limit Of Quantification ◽  
Surface Enhanced ◽  
Ph Range

The electrochemical behavior of isothipendyl hydrochloride (IPH) was investigated at bare and multiwalled-carbon-nanotube modified glassy carbon electrode (MWCNT-GCE). IPH (55 μM) showed two oxidation peaks in Britton-Robinson (BR) buffer of pH 7.0. The oxidation process of IPH was observed to be irreversible over the pH range of 2.5–9.0. The influence of pH, scan rate, and concentration of the drug on anodic peak was studied. A differential pulse voltammetric method with good precision and accuracy was developed for the determination of IPH in pure and biological fluids. The peak current was found to be linearly dependent on the concentration of IPH in the range of 1.25–55 μM. The values of limit of detection and limit of quantification were noticed to be 0.284 and 0.949 μM, respectively.

Core-shell Cu@C@ZIF-8 composite: a high-performance electrode material for electrochemical sensing of nitrite with high selectivity and sensitivity

2021 ◽  
Author(s):  
Feng Gao ◽  
Xiaolong Tu ◽  
Yongfang Yu ◽  
Yansha Gao ◽  
Jin Zou ◽  
...  
Keyword(s):  
High Performance ◽  
High Selectivity ◽  
Limit Of Detection ◽  
Metal Organic Framework ◽  
High Sensitivity ◽  
Electrochemical Sensing ◽  
Core Shell ◽  
Zeolitic Imidazolate Framework ◽  
Sensing Platform ◽  
The Difference

Abstract Herein, an efficient electrochemical sensing platform is proposed for selective and sensitive detection of nitrite on the basis of Cu@C@Zeolitic imidazolate framework-8 (Cu@C@ZIF-8) heterostructure. Core-shell Cu@C@ZIF-8 composite was synthesized by pyrolysis of Cu-metal-organic framework@ZIF-8 (Cu-MOF@ZIF-8) in Ar atmosphere on account of the difference of thermal stability between Cu-MOF and ZIF-8. For the sensing system of Cu@C@ZIF-8, ZIF-8 with proper pore size allows nitrite diffuse through the shell, while big molecules cannot, which ensures high selectivity of the sensor. On the other hand, Cu@C as electrocatalyst promotes the oxidation of nitrite, thereby resulting high sensitivity of the sensor. Accordingly, the Cu@C@ZIF-8 based sensor presents excellent performance for nitrite detection, which achieves a wide linear response range of 0.1 µM to 300.0 µM, and a low limit of detection (LOD) of 0.033 µM. In addition, the Cu@C@ZIF-8 sensor possesses excellent stability and reproducibility, and was employed to quantify nitrite in sausage samples with recoveries of 95.45-104.80%.

scholarly journals An Electrochemical Sensor Based on Gold Nanodendrite/Surfactant Modified Electrode for Bisphenol A Detection

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Nguyen Thi Lien ◽  
Le Quoc Hung ◽  
Nguyen Tien Hoang ◽  
Vu Thi Thu ◽  
Dau Thi Ngoc Nga ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Electrochemical Sensors ◽  
Differential Pulse ◽  
Electrochemical Sensing ◽  
Fluorescence Measurements ◽  
Sensing Platform ◽  
Electrochemical Window ◽  
Galvanostatic Deposition ◽  
Bisphenol A Detection ◽  
Good Agreement

In the present work, we reported the simple way to fabricate an electrochemical sensing platform to detect Bisphenol A (BPA) using galvanostatic deposition of Au on a glassy carbon electrode covered by cetyltrimethylammonium bromide (CTAB). This material (CTAB) enhances the sensitivity of electrochemical sensors with respect to the detection of BPA. The electrochemical response of the modified GCE to BPA was investigated by cyclic voltammetry and differential pulse voltammetry. The results displayed a low detection limit (22 nm) and a linear range from 0.025 to 10 µm along side with high reproducibility (RSD = 4.9% for seven independent sensors). Importantly, the prepared sensors were selective enough against interferences with other pollutants in the same electrochemical window. Notably, the presented sensors have already proven their ability in detecting BPA in real plastic water drinking bottle samples with high accuracy (recovery range = 96.60%–102.82%) and it is in good agreement with fluorescence measurements.

scholarly journals Carvacrol electrochemical reaction characteristics on screen printed electrode modified with La2O3/Co3O4 nanocomposite

2019 ◽  
Vol 9 (2) ◽  
pp. 113-123 ◽  
Author(s):  
Sayed Zia Mohammadi ◽  
Hadi Beitollahi ◽  
Tahereh Rohani ◽  
Hossein Allahabadi
Keyword(s):  
Limit Of Detection ◽  
Phosphate Buffer Solution ◽  
Differential Pulse ◽  
Co3o4 Nanoparticles ◽  
Electrochemical Characteristics ◽  
Screen Printed Electrode ◽  
Buffer Solution ◽  
Voltammetric Techniques ◽  
Screen Printed

Electrochemical characteristics of carvacrol were investigated on a screen-printed electrode (SPE) modified with La2O3/Co3O4 nanocomposite by using voltammetric techniques, which displayed a well-defined peak for sensitive carvacrol determination in phosphate buffer solution (PBS) at pH 7.0. La2O3/Co3O4 nanoparticles demonstrated suitable catalytic activity for carvacrol determination by differential pulse voltammetry (DPV) technique. Besides, determination of carvacrol in a real samples was recognized in the light of electrochemical findings and a validated voltammetric technique for quantitative analysis of carvacrol in a real formulation was proposed. The DPV peak currents were found to be linear in the concentration range of 10.0 to 800.0 μM. The limit of detection (LOD) was found to be 1.0 μM.

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