scholarly journals Bridging the gap between PCR detection of Mycobacterium tuberculosis complex and tuberculosis diagnosis [Short communication]

2012 ◽  
Vol 16 (3) ◽  
pp. 391-393 ◽  
Author(s):  
E. L. Duarte ◽  
E. Paixão ◽  
S. David
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Short Communication ◽  
Pcr Detection ◽  
Tuberculosis Diagnosis

scholarly journals Development and evaluation of a rapid multiplex-PCR based system for Mycobacterium tuberculosis diagnosis using sputum samples

2015 ◽  
Vol 116 ◽  
pp. 37-43 ◽  
Author(s):  
Isaac Mutingwende ◽  
Urban Vermeulen ◽  
Faans Steyn ◽  
Hendrik Viljoen ◽  
Anne Grobler
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Multiplex Pcr ◽  
Tuberculosis Diagnosis

scholarly journals Hasil Diagnostik Mycobacterium tuberculosis pada Pasien Batuk 2 Minggu dengan Pewarnaan Ziehl Neelsen di Poliklinik Interna RSU Pancaran Kasih Manado

2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Natasha M. Pomandia ◽  
Olivia A. Waworuntu ◽  
Heriyannis Homenta
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Positive Result ◽  
Gold Standard ◽  
Descriptive Study ◽  
Negative Results ◽  
Common Complaint ◽  
Contagious Disease ◽  
Tuberculosis Diagnosis

Abstract: Tuberculosis (TB) is a contagious disease caused by various strains of mycobacteria, commonly Mycobacterium tuberculosis. This disease can occur in children as well as in adults. The most common complaint in TB patients is cough. The gold standard of tuberculosis diagnosis is the culture of TB bacteria. However, Ziehl-Neelsen staining is the most frequently used method. It gives positive result if there are more than 103 bacteria per ml sputum. This study was aimed to find out the existence of M. tuberculosis in the sputum of patients coughed ≥2 weeks at the Internal Polyclinic of Pancaran Kasih Hospital Manado by using Ziehl-Neelsen staining. This was a descriptive study using 30 patients as samples. Of 30 samples, all (100%) had negative results of acid resistant bacili. Conclusion: In this study, all patients with cough ≥2 weeks in the Internal Polyclinic of Pancaran Kasih Hospital Manado had negative results of M. Tuberculosis based on Ziehl-Neelsen staining.Keywords: tuberculosis, cough ≥2 weeks Abstrak: Tuberkulosis (TB) ialah penyakit menular yang disebabkan oleh berbagai strain mikobakteria, umumnya Mycobacterium tuberculosis. Penyakit ini dapat terjadi pada anak atau orang dewasa. Keluhan yang sering ditemukan pada pasien TB ialah batuk. Dalam menegakkan diagnosis TB, baku emas pemeriksaan ialah kultur kuman TB. Selain kultur dapat digunakan metode pewarnaan basil tahan asam (BTA) Ziehl-Neelsen. Metode ini yang paling sering digunakan dan memberikan hasil positif bila terdapat lebih dari 103 kuman/ml sputum. Penelitian bertujuan untuk mengetahui ada tidaknya bakteri M. tuberculosis dengan menggunakan pewarnaan Ziehl-Neelsen pada pasien dengan batuk ≥2 minggu di Poliklinik Interna RSU Pancaran Kasih Manado. Jenis penelitian ialah deskriptif dengan mengunakan sebanyak 30 sampel. Hasil penelitian mendapatkan pada seluruh sampel (100%) tidak ditemukan BTA positif. Simpulan: Pada pasien dengan batuk ≥2 minggu di Poliklinik Interna RSU Pancaran Kasih Manado tidak ditemukan BTA positif berdasarkan pewarnaan Ziehl-Neelsen.Kata kunci: tuberkulosis (TBC), batuk ≥ 2 minggu

scholarly journals GAMBARAN BASIL TAHAN ASAM (BTA) POSITIF PADA PENDERITA DIAGNOSA KLINIS TUBERKULOSIS PARU DI RUMAH SAKIT ISLAM SITTI MARYAM MANADO PERIODE JANUARI 2014 S/D JUNI 2014

2014 ◽  
Vol 2 (2) ◽  
Author(s):  
Velma Buntuan
Keyword(s):  
Infectious Disease ◽  
Experimental Study ◽  
Mycobacterium Tuberculosis ◽  
Age Groups ◽  
Microscopical Examination ◽  
Age Group ◽  
Negative Results ◽  
Lung Tuberculosis ◽  
Tuberculosis Diagnosis ◽  
Positive Results

Absract: Tuberculosis is an acute or chronic infectious disease caused by Gram positive bacil, Mycobacterium tuberculosis, which predominantly infect the lungs. The diagnosis of lung tuberculosis in adults is based on the finding of these mycobacyteria in the microscopical examination of sputum. This was a descriptive experimental study to obtain the mycobacteria in patients’ sputum stained with Ziehl Neelsen method. Subjects were outpatients and inpatients clinically diagnosed as tuberculosis at the Internal Department of Sitti Maryam Hospital in Manado. The results showed that of 87 samples, there were 48 males (55%) and 39 females (45%). Age groups of 0-15 years consisted of 1 sample (1%); 16-20 years 0 sample (0%); 21-60 years 79 samples (91%); and >60 years 7 samples (8%). The microscopical examination showed positive results in 27 samples (37.0%) and negative results in 60 samples (69.0%). Conclusion: Mycobacterium tubercuosis infections were more frequent in males than in females. The most frequent age group was productive age, and the least frequent ages were children and teenagers. The number of positive results of sputum examination for Mycobacterium tuberculosis were less than negative results.Keywords: Mycobacterium tuberculosis, lung tuberculosis.Abstrak: Tuberkulosis paru adalah penyakit yang menular akut maupun kronis yang terutama menyerang paru, yang disebabkan oleh bakteri tahan asam (BTA) berbentuk batang yang bersifat Gram positif (Mycobacterium tuberculosis). Diagnosis tuberkulosis paru pada dewasa dapat ditegakkan dengan ditemukan BTA pada pemeriksaan mikroskopik dahak. Penelitian ini bersifat deskriptif ekperimental untuk menemukan kuman Mycobacterium tuberculosis pada sputum penderita dengan diagnosa klinis tuberkulosis di Poliklinik dan Rawat Inap Penyakit Dalam RSI Sitti Maryam Manado. Teknik pewarnaan BTA menggunakan metode Ziehl Neelsen. Hasil penelitian memperlihatkan dari 87 sampel, 48 sampel (55%) berjenis kelamin laki-laki dan 39 sampel (45%) berjenis kelamin perempuan. Jumlah sampel untuk kelompok usia 0-15 tahun sebesar 1 sampel (1%); 16-20 tahun 0 sampel (0%); 21-60 tahun 79 sampel (91%); dan >60 tahun 7 sampel (8%). Pada pemeriksaan sputum ditemukan BTA pada 27 sampel (37,0%) sedangkan pada 60 sampel (69,0%) tidak ditemukan. Simpulan: Pengidap tuberkulosis berjenis kelamin laki-laki lebih banyak dari pada perempuan, dengan kelompok usia tersering pada usia produktif dan paling sedikit pada usia anak dan remaja. Pemeriksaan sputum BTA positip lebih sedikit dibandingkan dengan BTA Negatip.Kata kunci: BTA, tuberkulosis paru.

scholarly journals Sensitivitas Metode Pemeriksaan Mikroskopis Fluorokrom dan Ziehl-Neelsen untuk Deteksi Mycobacterium tuberculosis pada Sputum

2019 ◽  
Vol 1 (2) ◽  
pp. 56
Author(s):  
BETTY SURYAWATI ◽  
LELI SAPTAWATI ◽  
ASTARI FEBYANE PUTRI ◽  
JATU APHRIDASARI
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Early Detection ◽  
Pulmonary Tuberculosis ◽  
Sensitivity And Specificity ◽  
Smear Microscopy ◽  
Microbiology Laboratory ◽  
Fluorochrome Staining ◽  
Primary Screening ◽  
Lowenstein Jensen ◽  
Tuberculosis Diagnosis

<p class="AbstractNormal"><em>Background: Detection of fast acid bacteria (FAB) using smear microscopy is used as a primary screening for tuberculosis diagnosis. Previous studies have shown that fluorochrome </em>(<em>Auroamine-rhodamine</em>) <em>staining showed better sensitivity compared to Ziehl-Neelsen (ZN) method in the detection of FAB in sputum. However this method has not been recommended for routine use including in Indonesia. This study aimed to evaluate the sensitivity and specificity of fluorochrome compared to ZN to detect FAB in patient’s sputum.</em><em></em></p><p class="AbstractNormal"><em>Methods: </em><em>This study analyzed 60 sputum samples from patients with tuberculosis and suspected pulmonary tuberculosis. Samples were obtained consecutively from microbiology laboratory</em><em> Moewardi Hospital, Indonesia. Each sample was examined using ZN and fluorochrome staining and cultured in Lowenstein-Jensen (LJ) medium.</em><em> Data were analyzed using sensitivity and spesificity tests.</em></p><p class="AbstractNormal"><em>Results: ZN staining detected FAB in 12 samples (10%), while fluorochrome detected FAB in 17 samples (28%). The sensitivity and specificity of ZN staining were 70% and 90% while these for fluorochrome were 90% and 84%. </em><em></em></p><p class="AbstractNormal"><em>Conclusions: The sensitivity of fluorochrome staining is better compared to ZN staining. This method can be recommended for early detection of tuberculosis.</em><em></em></p><p class="AbstractNormal"><em> </em></p>

PCR detection of co-infection with Mycobacterium tuberculosis and Mycobacterium avium in AIDS patients with meningitis

2012 ◽  
Vol 61 (12) ◽  
pp. 1789-1791 ◽  
Author(s):  
Kusum Sharma ◽  
Aman Sharma ◽  
Manish Modi ◽  
Gagandeep Singh ◽  
Harsimran Kaur ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Avium ◽  
Pcr Detection ◽  
Aids Patients
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